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PMID: 1990285 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Yeast CBP1 mRNA 3' end formation is regulated during the induction of mitochondrial function.

Molecular and cellular biology ·Vol. 11 ·No. 2 ·1991-02-00 ·Pages 813-21

Mayer SA, Dieckmann CL

Abstract

Alternative mRNA processing is one mechanism for generating two or more polypeptides from a single gene. While many mammalian genes contain multiple mRNA 3' cleavage and polyadenylation signals that change the coding sequence of the mature mRNA when used at different developmental stages or in different tissues, only one yeast gene has been identified with this capacity. The Saccharomyces cerevisiae nuclear gene CPB1 encodes a mitochondrial protein that is required for cytochrome b mRNA stability. This 66-kDa protein is encoded by a 2.2-kb mRNA transcribed from CPB1. Previously we showed that a second 1.2-kb transcript is initiated at the CBP1 promoter but has a 3' end near the middle of the coding sequence. Furthermore, it was shown that the ratio of the steady-state level of 2.2-kb CBP1 message to 1.2-kb message decreases 10-fold during the induction of mitochondrial function, while the combined levels of both messages remain constant. Having proposed that regulation of 3' end formation dictates the amount of each CBP1 transcript, we now show that a 146-bp fragment from the middle of CBP1 is sufficient to direct carbon source-regulated production of two transcripts when inserted into the yeast URA3 gene. This fragment contains seven polyadenylation sites for the wild-type 1.2-kb mRNA, as mapped by sequence analysis of CBP1 cDNA clones. Deletion mutations upstream of the polyadenylation sites abolished formation of the 1.2-kb transcript, whereas deletion of three of the sites only led to a reduction in abundance of the 1.2-kb mRNA. Our results indicate that regulation of the abundance of both CBP1 transcripts is controlled by elements in a short segment of the gene that directs 3' end formation of the 1.2-kb transcript, a unique case in yeast cells.

Related Genes
CBP1
MeSH Terms
Base Sequence Chromosome Deletion Cloning, Molecular Cytochrome b Group/genetics Escherichia coli/genetics Fungal Proteins/genetics Gene Expression Regulation, Fungal Gene Library Genes, Fungal Mitochondria/metabolism Molecular Sequence Data Oligonucleotide Probes Plasmids Polymerase Chain Reaction RNA, Messenger/genetics,metabolism Restriction Mapping Saccharomyces cerevisiae/genetics,metabolism Transcription, Genetic
Chemicals
Cytochrome b Group Fungal Proteins Oligonucleotide Probes RNA, Messenger
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mayer S A
Department of Biochemistry, University of Arizona, Tucson 85721.
Dieckmann C L
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.2.813-821.1991
Published
1991-02-00
Pages
813-21
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359733
Subset
IM
Grants
NIGMS NIH HHS · GM 34893 · United States
Databases
GENBANK
K02647, M64559, M64560, M64561, M64562, M64563, M64564, M64565, M64566, X59842
External Links
PubMed source DOI: 10.1128/mcb.11.2.813-821.1991 PMC Full Text View journal details
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