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PMID: 10074137 Published · ppublish English Journal Article

The ski7 antiviral protein is an EF1-alpha homolog that blocks expression of non-Poly(A) mRNA in Saccharomyces cerevisiae.

Journal of virology ·Vol. 73 ·No. 4 ·1999-04-00 ·Pages 2893-900

Benard L, Carroll K, Valle RC, Masison DC, Wickner RB

Abstract

We mapped and cloned SKI7, a gene that negatively controls the copy number of L-A and M double-stranded RNA viruses in Saccharomyces cerevisiae. We found that it encodes a nonessential 747-residue protein with similarities to two translation factors, Hbs1p and EF1-alpha. The ski7 mutant was hypersensitive to hygromycin B, a result also suggesting a role in translation. The SKI7 product repressed the expression of nonpolyadenylated [non-poly(A)] mRNAs, whether capped or uncapped, thus explaining why Ski7p inhibits the propagation of the yeast viruses, whose mRNAs lack poly(A). The dependence of the Ski7p effect on 3' RNA structures motivated a study of the expression of capped non-poly(A) luciferase mRNAs containing 3' untranslated regions (3'UTRs) differing in length. In a wild-type strain, increasing the length of the 3'UTR increased luciferase expression due to both increased rates and duration of translation. Overexpression of Ski7p efficiently cured the satellite virus M2 due to a twofold-increased repression of non-poly(A) mRNA expression. Our experiments showed that Ski7p is part of the Ski2p-Ski3p-Ski8p antiviral system because a single ski7 mutation derepresses the expression of non-poly(A) mRNA as much as a quadruple ski2 ski3 ski7 ski8 mutation, and the effect of the overexpression of Ski7p is not obtained unless other SKI genes are functional. ski1/xrn1Delta ski2Delta and ski1/xrn1Delta ski7Delta mutants were viable but temperature sensitive for growth.

MeSH Terms
Adaptor Proteins, Signal Transducing Amino Acid Sequence Cloning, Molecular Fungal Proteins/genetics GTP-Binding Proteins Gene Expression Regulation, Fungal Gene Expression Regulation, Viral Molecular Sequence Data RNA, Fungal/genetics RNA, Messenger/genetics Saccharomyces cerevisiae/genetics,virology Saccharomyces cerevisiae Proteins Sequence Alignment Transcription Factors/genetics
Chemicals
Adaptor Proteins, Signal Transducing Fungal Proteins RNA, Fungal RNA, Messenger SKI7 protein, S cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors GTP-Binding Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Benard L
Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892-0830, USA.
Carroll K
Valle R C
Masison D C
Wickner R B
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.73.4.2893-2900.1999
Published
1999-04-00
Pages
2893-900
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC104047
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.73.4.2893-2900.1999 PMC Full Text View journal details
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