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PMID: 18070923 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

Different requirements of the SWI/SNF complex for robust nucleosome displacement at promoters of heat shock factor and Msn2- and Msn4-regulated heat shock genes.

Molecular and cellular biology ·Vol. 28 ·No. 4 ·2008-02-00 ·Pages 1207-17

Erkina TY, Tschetter PA, Erkine AM

Abstract

The stress response in yeast cells is regulated by at least two classes of transcription activators-HSF and Msn2/4, which differentially affect promoter chromatin remodeling. We demonstrate that the deletion of SNF2, an ATPase activity-containing subunit of the chromatin remodeling SWI/SNF complex, eliminates histone displacement, RNA polymerase II recruitment, and heat shock factor (HSF) binding at the HSP12 promoter while delaying these processes at the HSP82 and SSA4 promoters. Out of the three promoters, the double deletion of MSN2 and MSN4 eliminates both chromatin remodeling and HSF binding only at the HSP12 promoter, suggesting that Msn2/4 activators are primary determinants of chromatin disassembly at the HSP12 promoter. Unexpectedly, during heat shock the level of Msn2/4 at the HSP12 promoter declines. This is likely a result of promoter-targeted Msn2/4 degradation associated with transcription complex assembly. While histone displacement kinetic profiles bear clear promoter specificity, the kinetic profiles of recovery from heat shock for all analyzed genes display an equal or even higher nucleosome return rate, which is to some extent delayed by the deletion of SNF2.

MeSH Terms
Chromatin Assembly and Disassembly Chromosomal Proteins, Non-Histone/metabolism DNA-Binding Proteins/deficiency,metabolism Gene Deletion Gene Expression Regulation, Fungal Genes, Fungal Heat-Shock Proteins/genetics Heat-Shock Response Kinetics Nucleosomes/metabolism Promoter Regions, Genetic/genetics Protein Binding RNA Polymerase II/metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/metabolism Transcription Factors/deficiency,metabolism Transcription, Genetic
Chemicals
Chromosomal Proteins, Non-Histone DNA-Binding Proteins Heat-Shock Proteins MSN2 protein, S cerevisiae MSN4 protein, S cerevisiae Nucleosomes SWI-SNF-B chromatin-remodeling complex Saccharomyces cerevisiae Proteins Transcription Factors RNA Polymerase II
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Erkina Tamara Y
Division of Basic Biomedical Sciences, University of South Dakota, Sanford School of Medicine, 414 E. Clark St., Vermillion, SD 57069, [email protected].
Tschetter Paul A
Erkine Alexandre M
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
1098-5549
DOI
10.1128/MCB.01069-07
Email
[email protected]
Published
2008-02-00
Epub
2007-00-10
Pages
1207-17
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC2258741
Subset
IM
Grants
NCRR NIH HHS · P20 RR016479 · United States
External Links
PubMed source DOI: 10.1128/MCB.01069-07 PMC Full Text View journal details
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