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PMID: 1833635 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

U4 small nuclear RNA dissociates from a yeast spliceosome and does not participate in the subsequent splicing reaction.

Molecular and cellular biology ·Vol. 11 ·No. 11 ·1991-11-00 ·Pages 5571-7

Yean SL, Lin RJ

Abstract

U4 and U6 small nuclear RNAs reside in a single ribonucleoprotein particle, and both are required for pre-mRNA splicing. The U4/U6 and U5 small nuclear ribonucleoproteins join U1 and U2 on the pre-mRNA during spliceosome assembly. Binding of U4 is then destabilized prior to or concomitant with the 5' cleavage-ligation. In order to test the role of U4 RNA, we isolated a functional spliceosome by using extracts prepared from yeast cells carrying a temperature-sensitive allele of prp2 (rna2). The isolated prp2 delta spliceosome contains U2, U5, U6, and possibly also U1 and can be activated to splice the bound pre-mRNA. U4 RNA does not associate with the isolated spliceosomes and is shown not to be involved in the subsequent cleavage-ligation reactions. These results are consistent with the hypothesis that the role of U4 in pre-mRNA splicing is to deliver U6 to the spliceosome.

MeSH Terms
Blotting, Northern RNA Precursors/genetics RNA Splicing RNA, Fungal/genetics,metabolism RNA, Small Nuclear/genetics,isolation & purification,metabolism Ribonucleoproteins/metabolism Ribonucleoproteins, Small Nuclear Saccharomyces cerevisiae/genetics
Chemicals
RNA Precursors RNA, Fungal RNA, Small Nuclear Ribonucleoproteins Ribonucleoproteins, Small Nuclear
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Yean S L
Department of Microbiology, University of Texas, Austin 78712-1095.
Lin R J
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32 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.11.5571-5577.1991
Published
1991-11-00
Pages
5571-7
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361927
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.11.11.5571-5577.1991 PMC Full Text View journal details
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