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PMID: 18445678 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Dnm1p-dependent peroxisome fission requires Caf4p, Mdv1p and Fis1p.

Journal of cell science ·Vol. 121 ·No. Pt 10 ·2008-05-15 ·Pages 1633-40

Motley AM, Ward GP, Hettema EH

Abstract

Yeast peroxisomes multiply by fission. Fission requires two dynamin-related proteins, Dnm1p and Vps1p. Using an in vivo fission assay, we show that Dnm1p-dependent peroxisome fission requires Fis1p, Caf4p and Mdv1p. Fluorescence microscopy of cells expressing GFP-tagged Caf4p and Mdv1p revealed that their association with peroxisomes relies on Fis1p. Vps1p-dependent peroxisome fission occurs independently of these factors. Vps1p contributes most to fission of peroxisomes when cells are grown on glucose. Overexpression of Dnm1p suppresses the fission defect as long as Fis1p and either Mdv1p or Caf4p are present. Conversely, overexpression of Dnm1p does not restore the vacuolar fusion defect of vps1 cells and Vps1p overexpression does not restore the mitochondrial fission defect of dnm1 cells. These data show that Vps1p and Dnm1p are part of independent fission machineries. Because the contribution of Dnm1p to peroxisome fission appears to be more pronounced in cells that proliferate peroxisomes in response to mitochondrial dysfunction, Dnm1p might be part of the mechanism that coordinates mitochondrial and peroxisomal biogenesis.

MeSH Terms
Adaptor Proteins, Signal Transducing Carrier Proteins/metabolism GTP Phosphohydrolases/metabolism GTP-Binding Proteins/metabolism Mitochondria/metabolism Mitochondrial Proteins/metabolism Peroxisomes/metabolism Saccharomyces cerevisiae/cytology,metabolism Saccharomyces cerevisiae Proteins/metabolism Vesicular Transport Proteins
Chemicals
Adaptor Proteins, Signal Transducing Caf4 protein, S cerevisiae Carrier Proteins FIS1 protein, S cerevisiae MDV1 protein, S cerevisiae Mitochondrial Proteins Saccharomyces cerevisiae Proteins Vesicular Transport Proteins GTP Phosphohydrolases GTP-Binding Proteins VPS1 protein, S cerevisiae DNM1 protein, S cerevisiae
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Motley Alison M
Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN, UK.
Ward Gemma P
Hettema Ewald H
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Article Info
Journal
Journal of cell science
Abbr.
J Cell Sci
ISSN
0021-9533
DOI
10.1242/jcs.026344
Published
2008-05-15
Epub
2008-00-29
Pages
1633-40
Language
English
Region
England
NLM ID
0052457
PMCID
PMC2579327
Subset
IM
Grants
Wellcome Trust · 073257 · United Kingdom
External Links
PubMed source DOI: 10.1242/jcs.026344 PMC Full Text View journal details
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