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PMID: 1870972 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification and sequence analysis of a methylase gene in Porphyromonas gingivalis.

Nucleic acids research ·Vol. 19 ·No. 15 ·1991-08-11 ·Pages 4189-92

Banas JA, Ferretti JJ, Progulske-Fox A

Abstract

A gene from the periodontal organism Porphyromonas gingivalis has been identified as encoding a DNA methylase. The gene, referred to as pgiIM, has been sequenced and found to contain a reading frame of 864 basepairs. The putative amino acid sequence of the encoded methylase was 288 amino acids, and shared 47% and 31% homology with the Streptococcus pneumoniae DpnII and E. coli Dam methylases, respectively. The activity and specificity of the pgi methylase (M.PgiI) was confirmed by cloning the gene into a dam- strain of E. coli (JM110) and performing a restriction analysis on the isolated DNA with enzymes whose activities depended upon the methylation state of the DNA. The data indicated that M.PgiI, like DpnII and Dam, methylated the adenine residue within the sequence 5'-GATC-3'.

Related Genes
pgilM
MeSH Terms
Amino Acid Sequence Bacteroides/enzymology,genetics Base Sequence Cloning, Molecular Escherichia coli/enzymology,genetics Molecular Sequence Data Open Reading Frames/genetics Sequence Homology, Nucleic Acid Site-Specific DNA-Methyltransferase (Adenine-Specific)/genetics Streptococcus pneumoniae/enzymology,genetics
Chemicals
Site-Specific DNA-Methyltransferase (Adenine-Specific)
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Banas J A
Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City 73190.
Ferretti J J
Progulske-Fox A
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21 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.15.4189
Published
1991-08-11
Pages
4189-92
Language
English
Region
England
NLM ID
0411011
PMCID
PMC328560
Subset
IM
Grants
NIDCR NIH HHS · DE05545 · United States
Databases
GENBANK
M63469
External Links
PubMed source DOI: 10.1093/nar/19.15.4189 PMC Full Text View journal details
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