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PMID: 3010047 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

DNA methylation differentially enhances the expression of one of the two E. coli dnaA promoters in vivo and in vitro.

Molecular & general genetics : MGG ·Vol. 202 ·No. 2 ·1986-02-00 ·Pages 246-50

Braun RE, Wright A

Abstract

The promoter/regulatory region of the dnaA gene, whose gene product is required for the initiation of DNA replication in Escherichia coli K-12, contains an unusually large number of Dam methylation sites. In this paper we report that the expression of the dnaA gene is decreased in Dam- strains of E. coli. The decrease in the expression of dnaA was measured in vivo using a dnaA-lacZ gene fusion. In vivo S1 nuclease mapping demonstrated that the decrease was due to a differential decrease in expression from the more proximal of the two dnaA promoters, dnaA2P. Comparison of the strengths of the two dnaA promoters in an in vitro transcription system using methylated and unmethylated DNA templates suggests that the effect of methylation on dnaA2P is probably at the level of RNA polymerase/DNA interaction. We suggest that this effect of methylation may be important in controlling the expression of dnaA during the E. coli cell cycle.

MeSH Terms
Bacterial Proteins/genetics Base Sequence DNA Restriction Enzymes Endonucleases Escherichia coli/genetics Genes Genes, Bacterial Genes, Regulator Methylation Promoter Regions, Genetic Single-Strand Specific DNA and RNA Endonucleases Transcription, Genetic
Chemicals
Bacterial Proteins Endonucleases DNA Restriction Enzymes Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Braun R E
Wright A
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34 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00331644
Published
1986-02-00
Pages
246-50
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00331644
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