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PMID: 18780728 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

ADBP-1 regulates an ADAR RNA-editing enzyme to antagonize RNA-interference-mediated gene silencing in Caenorhabditis elegans.

Genetics ·Vol. 180 ·No. 2 ·2008-10-00 ·Pages 785-96

Ohta H, Fujiwara M, Ohshima Y, Ishihara T

Abstract

Small interfering RNAs (siRNAs) and microRNAs (miRNAs) mediate gene silencing through evolutionarily conserved pathways. In Caenorhabditis elegans, the siRNA/miRNA pathways are also known to affect transgene expression. To identify genes that regulate the efficiencies of the siRNA/miRNA pathways, we used the expression level of a transgene as an indicator of gene silencing and isolated a transgene-silencing mutant, adbp-1 (ADR-2 binding protein). The adbp-1 mutation caused transgene silencing in hypodermal and intestinal cells in a cell-autonomous manner, depending on the RNA interference (RNAi) machinery. The adbp-1 gene encodes a protein with no conserved domains that is localized in the nucleus. Yeast two-hybrid screening and co-immunoprecipitation analysis demonstrated that ADBP-1 physically interacts with ADR-2, an RNA-editing enzyme from the ADAR (adenosine deaminase acting on dsRNA) family. In the adbp-1 mutant, as previously shown in adr-2 mutants, A-to-I RNA editing was not detected, suggesting that ADBP-1 is required for the RNA-editing activity of ADR-2. We found that ADBP-1 facilitates the nuclear localization of ADR-2. ADBP-1 may regulate ADR-2 activity and the consequent RNA editing and thereby antagonize RNAi-mediated transgene silencing in C. elegans.

MeSH Terms
Adenosine Deaminase/analysis,genetics,metabolism Animals Caenorhabditis elegans/enzymology,genetics,metabolism Caenorhabditis elegans Proteins/analysis,genetics,metabolism Carrier Proteins/genetics,metabolism Nuclear Proteins RNA Editing RNA Interference RNA, Helminth/genetics,metabolism RNA, Small Interfering Transgenes
Chemicals
ADBP-1 protein, C elegans Caenorhabditis elegans Proteins Carrier Proteins Nuclear Proteins RNA, Helminth RNA, Small Interfering Adenosine Deaminase Adr-2 protein, C elegans
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ohta Hiromitsu
Department of Applied Life Science, Sojo University, Kumamoto 860-0082, Japan.
Fujiwara Manabi
Ohshima Yasumi
Ishihara Takeshi
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1534/genetics.108.093310
Published
2008-10-00
Epub
2008-00-09
Pages
785-96
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC2567380
Subset
IM
External Links
PubMed source DOI: 10.1534/genetics.108.093310 PMC Full Text View journal details
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