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PMID: 18830797 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Activation tagging, an efficient tool for functional analysis of the rice genome.

Plant molecular biology ·Vol. 69 ·No. 1-2 ·2009-01-00 ·Pages 69-80

Wan S, Wu J, Zhang Z, Sun X, Lv Y, Gao C, Ning Y, Ma J, Guo Y, Zhang Q, Zheng X, Zhang C, Ma Z, Lu T

Abstract

Over the past 6 years, we have generated about 50,000 individual transgenic rice plants by an Agrobacterium-mediated transformation approach with the pER38 activation tagging vector. The vector contains tandemly arranged double 35S enhancers next to the right border of T-DNA. Expression analysis by reverse transcription-PCR indicates that the activation efficiency is high if the genes are located within 7 kb of the inserted double 35S enhancers. Comparative field phenotyping of part of the activation tagging and enhancer trapping populations in two generations (6,000 and 6,400 lines, respectively, in the T(0) generation, and 36,000 and 32,000 lines, respectively, in the T(1) generation) identified about four hundred dominant mutants. Characterization of a dominant mutant with a large leaf angle (M107) suggests that this mutant phenotype is caused by enhanced expression of CYP724B1/D11. The activation tagging pool described in this paper is a valuable alternative tool for functional analysis of the rice genome.

MeSH Terms
Base Sequence DNA Primers Genome, Plant Oryza/genetics Plants, Genetically Modified Polymerase Chain Reaction Rhizobium/genetics
Chemicals
DNA Primers
Authors & Affiliations
14 authors, click to expand affiliations / ORCID
Wan Shuyan
Biotechnology Research Institute/National Key Facility for Gene Resources and Gene Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Wu Jinxia
Zhang Zhiguo
Sun Xuehui
Lv Yaci
Gao Ci
Ning Yingda
Ma Jun
Guo Yupeng
Zhang Qian
Zheng Xia
Zhang Caiying
Ma Zhiying
Lu Tiegang
References (44)
44 references, click to expand
  1. Functional genomics in Arabidopsis: large-scale insertional mutagenesis complements the genome sequencing project.
    Curr Opin Biotechnol. 2000 Apr;11(2):157-61 PMID: 10753770
  2. The Arabidopsis knockout facility at the University of Wisconsin-Madison.
    Plant Physiol. 2000 Dec;124(4):1465-7 PMID: 11115860
  3. BRS1, a serine carboxypeptidase, regulates BRI1 signaling in Arabidopsis thaliana.
    Proc Natl Acad Sci U S A. 2001 May 8;98(10):5916-21 PMID: 11320207
  4. High throughput T-DNA insertion mutagenesis in rice: a first step towards in silico reverse genetics.
    Plant J. 2004 Aug;39(3):450-64 PMID: 15255873
  5. Analysis of flanking sequences from dissociation insertion lines: a database for reverse genetics in Arabidopsis.
    Plant Cell. 1999 Dec;11(12):2263-70 PMID: 10590156
  6. A rice phenomics study--phenotype scoring and seed propagation of a T-DNA insertion-induced rice mutant population.
    Plant Mol Biol. 2007 Nov;65(4):427-38 PMID: 17701278
  7. Rice mutant resources for gene discovery.
    Plant Mol Biol. 2004 Feb;54(3):325-34 PMID: 15284490
  8. Activation tagging in Arabidopsis.
    Plant Physiol. 2000 Apr;122(4):1003-13 PMID: 10759496
  9. Rapid isolation of high molecular weight plant DNA.
    Nucleic Acids Res. 1980 Oct 10;8(19):4321-5 PMID: 7433111
  10. A high-throughput Arabidopsis reverse genetics system.
    Plant Cell. 2002 Dec;14(12):2985-94 PMID: 12468722
  11. Development of enhancer trap lines for functional analysis of the rice genome.
    Plant J. 2003 Aug;35(3):418-27 PMID: 12887592
  12. Retrotransposons of rice involved in mutations induced by tissue culture.
    Proc Natl Acad Sci U S A. 1996 Jul 23;93(15):7783-8 PMID: 8755553
  13. Activation tagging identifies a conserved MYB regulator of phenylpropanoid biosynthesis.
    Plant Cell. 2000 Dec;12(12):2383-2394 PMID: 11148285
  14. Isolation and molecular characterization of a Spotted leaf 18 mutant by modified activation-tagging in rice.
    Plant Mol Biol. 2007 Apr;63(6):847-60 PMID: 17273822
  15. Generation of a flanking sequence-tag database for activation-tagging lines in japonica rice.
    Plant J. 2006 Jan;45(1):123-32 PMID: 16367959
  16. A two-component enhancer-inhibitor transposon mutagenesis system for functional analysis of the Arabidopsis genome.
    Plant Cell. 1999 Oct;11(10):1853-66 PMID: 10521517
  17. Activation tagging of the two closely linked genes LEP and VAS independently affects vascular cell number.
    Plant J. 2002 Dec;32(5):819-30 PMID: 12472696
  18. Large-scale characterization of Tos17 insertion sites in a rice T-DNA mutant library.
    Plant Mol Biol. 2007 Nov;65(5):587-601 PMID: 17874225
  19. Target site specificity of the Tos17 retrotransposon shows a preference for insertion within genes and against insertion in retrotransposon-rich regions of the genome.
    Plant Cell. 2003 Aug;15(8):1771-80 PMID: 12897251
  20. A rice gene activation/knockout mutant resource for high throughput functional genomics.
    Plant Mol Biol. 2007 Feb;63(3):351-64 PMID: 17120135
  21. T-DNA insertional mutagenesis for functional genomics in rice.
    Plant J. 2000 Jun;22(6):561-70 PMID: 10886776
  22. A novel cytochrome P450 is implicated in brassinosteroid biosynthesis via the characterization of a rice dwarf mutant, dwarf11, with reduced seed length.
    Plant Cell. 2005 Mar;17(3):776-90 PMID: 15705958
  23. Activation tagging of a dominant gibberellin catabolism gene (GA 2-oxidase) from poplar that regulates tree stature.
    Plant Physiol. 2003 Jul;132(3):1283-91 PMID: 12857810
  24. T-DNA insertional mutagenesis for activation tagging in rice.
    Plant Physiol. 2002 Dec;130(4):1636-44 PMID: 12481047
  25. Large-scale enhancer detection in the zebrafish genome.
    Development. 2005 Sep;132(17):3799-811 PMID: 16049110
  26. Contribution of the Tos17 retrotransposon to rice functional genomics.
    Curr Opin Plant Biol. 2001 Apr;4(2):118-22 PMID: 11228433
  27. Activation tagging of the LEAFY PETIOLE gene affects leaf petiole development in Arabidopsis thaliana.
    Development. 2000 Nov;127(22):4971-80 PMID: 11044410
  28. BAS1: A gene regulating brassinosteroid levels and light responsiveness in Arabidopsis.
    Proc Natl Acad Sci U S A. 1999 Dec 21;96(26):15316-23 PMID: 10611382
  29. Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA.
    Plant J. 1994 Aug;6(2):271-82 PMID: 7920717
  30. Multiple independent defective suppressor-mutator transposon insertions in Arabidopsis: a tool for functional genomics.
    Plant Cell. 1999 Oct;11(10):1841-52 PMID: 10521516
  31. Activation tagging using the En-I maize transposon system in Arabidopsis.
    Plant Physiol. 2002 Aug;129(4):1544-56 PMID: 12177467
  32. Comparative analysis of the receptor-like kinase family in Arabidopsis and rice.
    Plant Cell. 2004 May;16(5):1220-34 PMID: 15105442
  33. Chromatin structure can strongly facilitate enhancer action over a distance.
    Proc Natl Acad Sci U S A. 2006 Nov 21;103(47):17690-5 PMID: 17101994
  34. CKI1, a histidine kinase homolog implicated in cytokinin signal transduction.
    Science. 1996 Nov 8;274(5289):982-5 PMID: 8875940
  35. Highly efficient production and characterization of T-DNA plants for rice ( Oryza sativa L.) functional genomics.
    Theor Appl Genet. 2003 May;106(8):1396-408 PMID: 12677401
  36. T-DNA as an insertional mutagen in Arabidopsis.
    Plant Cell. 1999 Dec;11(12):2283-90 PMID: 10590158
  37. BAK1, an Arabidopsis LRR receptor-like protein kinase, interacts with BRI1 and modulates brassinosteroid signaling.
    Cell. 2002 Jul 26;110(2):213-22 PMID: 12150929
  38. A Dissociation insertion causes a semidominant mutation that increases expression of TINY, an Arabidopsis gene related to APETALA2.
    Plant Cell. 1996 Apr;8(4):659-71 PMID: 8624440
  39. A barley activation tagging system.
    Plant Mol Biol. 2007 Jun;64(3):329-47 PMID: 17429742
  40. Activation tagging in tomato identifies a transcriptional regulator of anthocyanin biosynthesis, modification, and transport.
    Plant Cell. 2003 Aug;15(8):1689-703 PMID: 12897245
  41. Sequence database of 1172 T-DNA insertion sites in Arabidopsis activation-tagging lines that showed phenotypes in T1 generation.
    Plant J. 2003 Nov;36(3):421-9 PMID: 14617098
  42. Erect leaves caused by brassinosteroid deficiency increase biomass production and grain yield in rice.
    Nat Biotechnol. 2006 Jan;24(1):105-9 PMID: 16369540
  43. Activation tagging, a novel tool to dissect the functions of a gene family.
    Plant J. 2003 Jun;34(5):741-50 PMID: 12787254
  44. T-DNA insertion mutagenesis in Arabidopsis: going back and forth.
    Trends Genet. 1997 Apr;13(4):152-6 PMID: 9097726
Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/s11103-008-9406-5
Published
2009-01-00
Epub
2008-00-02
Pages
69-80
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
External Links
PubMed source DOI: 10.1007/s11103-008-9406-5 View journal details
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