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PMID: 1896013 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The superoperonal organization of genes for pigment biosynthesis and reaction center proteins is a conserved feature in Rhodobacter capsulatus: analysis of overlapping bchB and puhA transcripts.

Molecular & general genetics : MGG ·Vol. 228 ·No. 3 ·1991-09-00 ·Pages 433-44

Bauer CE, Buggy JJ, Yang ZM, Marrs BL

Abstract

Most of the essential biosynthetic and structural genes involved in bacterial photosynthesis are clustered in a 46 kb region of the Rhodobacter capsulatus genome. Previous analyses have demonstrated that the puf operon, which encodes light harvesting and reaction center structural genes as well as a regulatory gene for bacteriochlorophyll biosynthesis, is expressed from a complex set of overlapping transcripts. Differential initiation and processing of these transcripts is thought to be involved in regulating expression of puf-encoded genes. In this study we demonstrate that the puh operon, which is located 39 kb away from the puf operon, also contains overlapping transcripts. One large 11 kb puhA transcript is shown to be a product of read-through from an upstream operon (bchB) which encodes numerous bacteriochlorophyll biosynthesis genes. A second 1.1 kb mRNA is shown to be derived from the 11 kb bchB transcript by processing and a third, highly expressed, 0.95 kb transcript is shown to be initiated from a promoter located within the distal gene of the bchB operon. The occurrence of overlapping transcripts for the puf and puh operons was further shown to influence development of the photochemical apparatus during conditions of environmental shifts in oxygen tension. Evidence for the occurrence of a "superoperonal" organization of overlapping operons in several different species of purple photosynthetic bacteria is discussed.

Related Genes
bchB puhA
MeSH Terms
Amino Acid Sequence Bacterial Proteins Bacteriochlorophylls/biosynthesis,genetics Base Sequence Electrophoresis, Polyacrylamide Gel Genes, Bacterial Light-Harvesting Protein Complexes Molecular Sequence Data Multigene Family Operon Photosynthetic Reaction Center Complex Proteins/genetics,metabolism Pigments, Biological/biosynthesis Promoter Regions, Genetic Rhodobacter capsulatus/genetics Sequence Alignment Transcription, Genetic
Chemicals
Bacterial Proteins Bacteriochlorophylls Light-Harvesting Protein Complexes Photosynthetic Reaction Center Complex Proteins Pigments, Biological PuhA protein, Rhodobacter
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bauer C E
Department of Biology, Indiana University, Bloomington 47405.
Buggy J J
Yang Z M
Marrs B L
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48 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00260637
Published
1991-09-00
Pages
433-44
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · R01 GM040941 · United States
NIGMS NIH HHS · GM40941-01 · United States
External Links
PubMed source DOI: 10.1007/BF00260637
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