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PMID: 19020518 Published · ppublish English Comparative Study Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Recognition of aminoacyl-tRNA: a common molecular mechanism revealed by cryo-EM.

The EMBO journal ·Vol. 27 ·No. 24 ·2008-12-17 ·Pages 3322-31

Li W, Agirrezabala X, Lei J, Bouakaz L, Brunelle JL, Ortiz-Meoz RF, Green R, Sanyal S, Ehrenberg M, Frank J

Abstract

The accuracy of ribosomal translation is achieved by an initial selection and a proofreading step, mediated by EF-Tu, which forms a ternary complex with aminoacyl(aa)-tRNA. To study the binding modes of different aa-tRNAs, we compared cryo-EM maps of the kirromycin-stalled ribosome bound with ternary complexes containing Phe-tRNA(Phe), Trp-tRNA(Trp), or Leu-tRNA(LeuI). The three maps suggest a common binding manner of cognate aa-tRNAs in their specific binding with both the ribosome and EF-Tu. All three aa-tRNAs have the same 'loaded spring' conformation with a kink and twist between the D-stem and anticodon stem. The three complexes are similarly integrated in an interaction network, extending from the anticodon loop through h44 and protein S12 to the EF-Tu-binding CCA end of aa-tRNA, proposed to signal cognate codon-anticodon interaction to the GTPase centre and tune the accuracy of aa-tRNA selection.

MeSH Terms
Cryoelectron Microscopy Models, Molecular Nucleic Acid Conformation Peptide Elongation Factor Tu/chemistry,metabolism Protein Structure, Quaternary Protein Synthesis Inhibitors/pharmacology Pyridones/pharmacology RNA, Transfer/chemistry,metabolism Ribosomes/chemistry,metabolism
Chemicals
Protein Synthesis Inhibitors Pyridones RNA, Transfer Peptide Elongation Factor Tu mocimycin
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Li Wen
Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.
Agirrezabala Xabier
Lei Jianlin
Bouakaz Lamine
Brunelle Julie L
Ortiz-Meoz Rodrigo F
Green Rachel
Sanyal Suparna
Ehrenberg Måns
Frank Joachim
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
1460-2075
DOI
10.1038/emboj.2008.243
Published
2008-12-17
Epub
2008-00-20
Pages
3322-31
Language
English
Region
England
NLM ID
8208664
PMCID
PMC2586802
Subset
IM
Grants
NIGMS NIH HHS · R01 GM55440 · United States
NIGMS NIH HHS · R01 GM070768 · United States
NIGMS NIH HHS · R01 GM059425 · United States
NIGMS NIH HHS · R01 GM055440 · United States
NIGMS NIH HHS · GM70768 · United States
NIGMS NIH HHS · R37 GM029169 · United States
NIGMS NIH HHS · R37 GM29169 · United States
External Links
PubMed source DOI: 10.1038/emboj.2008.243 PMC Full Text View journal details
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