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PMID: 19104067 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Review

Imaging the glycome.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 106 ·No. 1 ·2009-01-06 ·Pages 12-7

Laughlin ST, Bertozzi CR

Abstract

Molecular imaging enables visualization of specific molecules in vivo and without substantial perturbation to the target molecule's environment. Glycans are appealing targets for molecular imaging but are inaccessible with conventional approaches. Classic methods for monitoring glycans rely on molecular recognition with probe-bearing lectins or antibodies, but these techniques are not well suited to in vivo imaging. In an emerging strategy, glycans are imaged by metabolic labeling with chemical reporters and subsequent ligation to fluorescent probes. This technique has enabled visualization of glycans in living cells and in live organisms such as zebrafish. Molecular imaging with chemical reporters offers a new avenue for probing changes in the glycome that accompany development and disease.

MeSH Terms
Animals Cells/chemistry Diagnostic Imaging Humans Molecular Probe Techniques Polysaccharides/analysis
Chemicals
Polysaccharides
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Laughlin Scott T
Department of Chemistry, University of California, Berkeley, CA 94720, USA.
Bertozzi Carolyn R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
1091-6490
DOI
10.1073/pnas.0811481106
Published
2009-01-06
Epub
2008-00-22
Pages
12-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC2629201
Subset
IM
Grants
NIGMS NIH HHS · R01 GM058867 · United States
NIGMS NIH HHS · R37 GM058867 · United States
Howard Hughes Medical Institute · United States
NIGMS NIH HHS · GM58867 · United States
External Links
PubMed source DOI: 10.1073/pnas.0811481106 PMC Full Text View journal details
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