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PMID: 19158388 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Esc2 and Sgs1 act in functionally distinct branches of the homologous recombination repair pathway in Saccharomyces cerevisiae.

Molecular biology of the cell ·Vol. 20 ·No. 6 ·2009-03-00 ·Pages 1683-94

Mankouri HW, Ngo HP, Hickson ID

Abstract

Esc2 is a member of the RENi family of SUMO-like domain proteins and is implicated in gene silencing in Saccharomyces cerevisiae. Here, we identify a dual role for Esc2 during S-phase in mediating both intra-S-phase DNA damage checkpoint signaling and preventing the accumulation of Rad51-dependent homologous recombination repair (HRR) intermediates. These roles are qualitatively similar to those of Sgs1, the yeast ortholog of the human Bloom's syndrome protein, BLM. However, whereas mutation of either ESC2 or SGS1 leads to the accumulation of unprocessed HRR intermediates in the presence of MMS, the accumulation of these structures in esc2 (but not sgs1) mutants is entirely dependent on Mph1, a protein that shows structural similarity to the Fanconi anemia group M protein (FANCM). In the absence of both Esc2 and Sgs1, the intra-S-phase DNA damage checkpoint response is compromised after exposure to MMS, and sgs1esc2 cells attempt to undergo mitosis with unprocessed HRR intermediates. We propose a model whereby Esc2 acts in an Mph1-dependent process, separately from Sgs1, to influence the repair/tolerance of MMS-induced lesions during S-phase.

MeSH Terms
Cell Cycle Proteins/genetics,metabolism Checkpoint Kinase 2 DEAD-box RNA Helicases/genetics,metabolism DNA Damage/genetics DNA Repair/genetics DNA, Fungal/genetics,metabolism Genomic Instability/genetics Mitosis Mutation/genetics Nuclear Proteins/genetics,metabolism Protein Serine-Threonine Kinases/genetics,metabolism RecQ Helicases/genetics,metabolism Recombination, Genetic/genetics Saccharomyces cerevisiae/cytology,genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism
Chemicals
Cell Cycle Proteins DNA, Fungal Esc2 protein, S cerevisiae Nuclear Proteins Saccharomyces cerevisiae Proteins Checkpoint Kinase 2 Protein Serine-Threonine Kinases RAD53 protein, S cerevisiae MPH1 protein, S cerevisiae SGS1 protein, S cerevisiae RecQ Helicases DEAD-box RNA Helicases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mankouri Hocine W
Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DS, United Kingdom.
Ngo Hien-Ping
Hickson Ian D
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1939-4586
Published
2009-03-00
Epub
2009-00-21
Pages
1683-94
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC2655263
Subset
IM
Grants
Cancer Research UK · United Kingdom
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