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PMID: 1923804 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A DNA binding domain is contained in the C-terminus of wild type p53 protein.

Nucleic acids research ·Vol. 19 ·No. 19 ·1991-10-11 ·Pages 5191-8

Foord OS, Bhattacharya P, Reich Z, Rotter V

Abstract

In the present study we evaluated the DNA binding activity of wild type and mutant p53 proteins that were isolated from bacterial expression vectors. A comparison of the binding activities of the various purified p53 proteins, assessed by their ability to bind DNA cellulose columns, indicated that wild type p53 has a higher affinity to DNA than have mutant p53 forms. Furthermore, only wild type p53 was able to bind genomic DNA upon electrophoretic protein blotting. As specific deletion of the C-terminal region of wild type p53 totally abolished binding to genomic DNA, it was concluded that the 47 C-terminal amino acids contain the DNA binding region. The fact that the N-terminus contains a transcription activation region whereas the C-terminus contains a DNA binding domain places p53 in the family of typical transcription factors. Our experiments show that the topographical positioning of these domains plays an important role in the activity of wild type p53.

MeSH Terms
Amino Acid Sequence Animals Binding Sites Blotting, Western Chromatography, DEAE-Cellulose DNA/metabolism DNA Mutational Analysis Escherichia coli/metabolism HeLa Cells Humans Mice Molecular Sequence Data Plasmids/genetics Protein Conformation Transcription, Genetic/physiology Transfection/genetics Tumor Suppressor Protein p53/biosynthesis,chemistry,genetics,metabolism
Chemicals
Tumor Suppressor Protein p53 DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Foord O S
Department of Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
Bhattacharya P
Reich Z
Rotter V
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.19.5191
Published
1991-10-11
Pages
5191-8
Language
English
Region
England
NLM ID
0411011
PMCID
PMC328875
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/19.19.5191 PMC Full Text View journal details
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