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PMID: 19282969 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

A fine-structure map of spontaneous mitotic crossovers in the yeast Saccharomyces cerevisiae.

PLoS genetics ·Vol. 5 ·No. 3 ·2009-03-00 ·Pages e1000410

Lee PS, Greenwell PW, Dominska M, Gawel M, Hamilton M, Petes TD

Abstract

Homologous recombination is an important mechanism for the repair of DNA damage in mitotically dividing cells. Mitotic crossovers between homologues with heterozygous alleles can produce two homozygous daughter cells (loss of heterozygosity), whereas crossovers between repeated genes on non-homologous chromosomes can result in translocations. Using a genetic system that allows selection of daughter cells that contain the reciprocal products of mitotic crossing over, we mapped crossovers and gene conversion events at a resolution of about 4 kb in a 120-kb region of chromosome V of Saccharomyces cerevisiae. The gene conversion tracts associated with mitotic crossovers are much longer (averaging about 12 kb) than the conversion tracts associated with meiotic recombination and are non-randomly distributed along the chromosome. In addition, about 40% of the conversion events have patterns of marker segregation that are most simply explained as reflecting the repair of a chromosome that was broken in G1 of the cell cycle.

MeSH Terms
Chromosome Mapping Chromosomes, Fungal DNA Repair/genetics G1 Phase Mitosis/genetics Recombination, Genetic Saccharomyces cerevisiae/cytology,genetics
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Lee Phoebe S
Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina, United States of America.
Greenwell Patricia W
Dominska Margaret
Gawel Malgorzata
Hamilton Monica
Petes Thomas D
Conflict of Interest

The authors have declared that no competing interests exist.

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Article Info
Journal
PLoS genetics
Abbr.
PLoS Genet
ISSN
1553-7404
DOI
10.1371/journal.pgen.1000410
Published
2009-03-00
Epub
2009-00-13
Pages
e1000410
Language
English
Region
United States
NLM ID
101239074
PMCID
PMC2646836
Subset
IM
Grants
NIGMS NIH HHS · R01 GM024110 · United States
NIGMS NIH HHS · R01 GM052319 · United States
NIGMS NIH HHS · GM24110 · United States
NIGMS NIH HHS · GM52319 · United States
Corrections
CommentIn
PMID: 19282976
External Links
PubMed source DOI: 10.1371/journal.pgen.1000410 PMC Full Text View journal details
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