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PMID: 1938927 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning and structural characterization of the mcrA locus of Escherichia coli.

Journal of bacteriology ·Vol. 173 ·No. 22 ·1991-11-00 ·Pages 7368-73

Hiom K, Sedgwick SG

Abstract

Escherichia coli has DNA restriction systems which are able to recognize and attack modified cytosine residues in the DNA of incoming bacteriophages and plasmids. The locus for the McrA/RglA system of modified cytosine restriction was located near the pin gene of the defective element, e14. Hence, loss of the e14 element through abortive induction after UV irradiation caused a permanent loss of McrA restriction activity. e14 DNA encoding McrA restriction was cloned and sequenced to reveal a single open reading frame of 831 bp with a predicted gene product of 31 kDa. Clones expressing the complete open reading frame conferred both McrA and RglA phenotypes; however, a deletion derivative was found which complemented RglA restriction against nonglucosylated T6gt phage but did not complement for McrA restriction of methylated plasmid DNA. Possible explanations for this activity and a comparison with the different organization of the McrB/RglB restriction system are discussed.

Related Genes
mcrA
MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular/methods DNA, Bacterial/genetics,isolation & purification Escherichia coli/genetics,growth & development,radiation effects Genes, Bacterial Genetic Complementation Test Molecular Sequence Data Phenotype Plasmids Restriction Mapping Transformation, Bacterial/radiation effects Ultraviolet Rays
Chemicals
DNA, Bacterial
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hiom K
Genetics Division, National Institute for Medical Research, Mill Hill, London, Great Britain.
Sedgwick S G
References (36)
36 references, click to expand
  1. Host specificity of DNA produced by Escherichia coli: bacterial mutations affecting the restriction and modification of DNA.
    J Mol Biol. 1966 Mar;16(1):118-33 PMID: 5331240
  2. MUTANTS OF BACTERIOPHAGES T2 AND T6 DEFECTIVE IN ALPHA-GLUCOSYL TRANSFERASE.
    Biochem Biophys Res Commun. 1965 Feb 17;18:545-50 PMID: 14301458
  3. UV-induced alleviation of K-specific restriction of bacteriophage lambda.
    J Virol. 1977 Mar;21(3):1249-51 PMID: 321803
  4. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  5. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  6. Simple method for identification of plasmid-coded proteins.
    J Bacteriol. 1979 Jan;137(1):692-3 PMID: 368040
  7. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  8. Identification of a novel genetic element in Escherichia coli K-12.
    J Bacteriol. 1980 Oct;144(1):312-21 PMID: 6998954
  9. UV-induced allevation of lambda restriction in Escherichia coli K-12: kinetics of induction and specificity of this SOS function.
    Mol Gen Genet. 1982;186(1):111-7 PMID: 6213835
  10. An improved method for preparing large arrays of bacterial colonies containing plasmids for hybridization: in situ purification and stable binding of DNA on paper filters.
    Anal Biochem. 1982 Oct;126(1):222-30 PMID: 6295209
  11. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  12. A Mu gin complementing function and an invertible DNA region in Escherichia coli K-12 are situated on the genetic element e14.
    J Bacteriol. 1984 May;158(2):517-22 PMID: 6233259
  13. Transposon mutagenesis and genetic mapping of the rglA and rglB loci of Escherichia coli.
    Mol Gen Genet. 1985;198(3):390-2 PMID: 2989655
  14. The invertible P-DNA segment in the chromosome of Escherichia coli.
    EMBO J. 1985 Jan;4(1):237-42 PMID: 3894006
  15. Acceleration of nucleic acid hybridization rate by polyethylene glycol.
    Anal Biochem. 1986 Feb 1;152(2):304-7 PMID: 2421601
  16. Escherichia coli K-12 restricts DNA containing 5-methylcytosine.
    Proc Natl Acad Sci U S A. 1986 Dec;83(23):9070-4 PMID: 3024165
  17. Localization of a genetic region involved in McrB restriction by Escherichia coli K-12.
    J Bacteriol. 1987 Apr;169(4):1757-9 PMID: 3031021
  18. Cytosine-specific DNA modification interferes with plasmid establishment in Escherichia coli K12: involvement of rglB.
    Mol Gen Genet. 1986 Dec;205(3):469-75 PMID: 3550384
  19. The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library.
    Cell. 1987 Jul 31;50(3):495-508 PMID: 3038334
  20. Restriction and modification in vivo by Escherichia coli K12.
    Methods Enzymol. 1987;152:130-41 PMID: 2821354
  21. McrA and McrB restriction phenotypes of some E. coli strains and implications for gene cloning.
    Nucleic Acids Res. 1988 Feb 25;16(4):1563-75 PMID: 2831502
  22. Characterization of the Escherichia coli modified cytosine restriction (mcrB) gene.
    Gene. 1987;61(3):277-89 PMID: 2833428
  23. Attachment site of the genetic element e14.
    J Bacteriol. 1988 May;170(5):2040-4 PMID: 3283103
  24. Nucleotide sequence of the McrB region of Escherichia coli K-12 and evidence for two independent translational initiation sites at the mcrB locus.
    J Bacteriol. 1989 Apr;171(4):1974-81 PMID: 2649480
  25. Genetic dissection of the methylcytosine-specific restriction system mcrB of Escherichia coli K-12.
    Gene. 1988 Dec 25;74(1):23-4 PMID: 2854808
  26. Construction of a temperature-sensitive mutation for the direct identification of plasmids encoding DNA methyltransferases.
    Gene. 1988 Dec 25;74(1):233-5 PMID: 2854809
  27. Identification of a second polypeptide required for McrB restriction of 5-methylcytosine-containing DNA in Escherichia coli K12.
    Mol Gen Genet. 1989 Apr;216(2-3):402-7 PMID: 2664457
  28. Genetic and physical mapping of the mcrA (rglA) and mcrB (rglB) loci of Escherichia coli K-12.
    Genetics. 1989 Jun;122(2):279-96 PMID: 2548920
  29. Nucleotide excision repair in Escherichia coli.
    Microbiol Rev. 1990 Mar;54(1):18-51 PMID: 2181258
  30. Genetic and sequence organization of the mcrBC locus of Escherichia coli K-12.
    J Bacteriol. 1990 Sep;172(9):4888-900 PMID: 2203735
  31. Isolation of temperature-sensitive McrA and McrB mutations and complementation analysis of the McrBC region of Escherichia coli K-12.
    J Bacteriol. 1991 Jan;173(1):150-5 PMID: 1987114
  32. Nomenclature relating to restriction of modified DNA in Escherichia coli.
    J Bacteriol. 1991 Apr;173(8):2707-9 PMID: 2013582
  33. Different mechanisms for SOS induced alleviation of DNA restriction in Escherichia coli.
    Biochimie. 1991 Apr;73(4):399-405 PMID: 1655051
  34. A nonhereditary, host-induced variation of bacterial viruses.
    J Bacteriol. 1952 Oct;64(4):557-69 PMID: 12999684
  35. A LOCUS THAT CONTROLS FILAMENT FORMATION AND SENSITIVITY TO RADIATION IN ESCHERICHIA COLI K-12.
    Genetics. 1964 Feb;49:237-46 PMID: 14124942
  36. Restriction of nonglucosylated T-even bacteriophage: properties of permissive mutants of Escherichia coli B and K12.
    Virology. 1967 Apr;31(4):688-701 PMID: 4290282
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.22.7368-7373.1991
Published
1991-11-00
Pages
7368-73
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC209246
Subset
IM
Databases
GENBANK
M63255, M64327, M64328, M64329, M64330, M64331, M64332, M76667, S64950, S64997, S64999
External Links
PubMed source DOI: 10.1128/jb.173.22.7368-7373.1991 PMC Full Text View journal details
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