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PMID: 2649480 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence of the McrB region of Escherichia coli K-12 and evidence for two independent translational initiation sites at the mcrB locus.

Journal of bacteriology ·Vol. 171 ·No. 4 ·1989-04-00 ·Pages 1974-81

Ross TK, Achberger EC, Braymer HD

Abstract

The McrB restriction system of Escherichia coli K-12 is responsible for the biological inactivation of foreign DNA that contains 5-methylcytosine residues (E. A. Raleigh and G. Wilson, Proc. Natl. Acad. Sci. USA 83:9070-9074, 1986). Within the McrB region of the chromosome is the mcrB gene, which encodes a protein of 51 kilodaltons (kDa) (T. K. Ross, E. C. Achberger, and H. D. Braymer, Gene 61:277-289, 1987), and the mcrC gene, the product of which is 39 kDa (T. K. Ross, E. C. Achberger, and H. D. Braymer, Mol. Gen. Genet., in press). The nucleotide sequence of a 2,695-base-pair segment encompassing the McrB region was determined. The deduced amino acid sequence was used to identify two open reading frames specifying peptides of 455 and 348 amino acids, corresponding to the products of the mcrB and mcrC genes, respectively. A single-nucleotide overlap was found to exist between the termination codon of the mcrB gene and the proposed initiation codon of the mcrC gene. The presence of an additional peptide of 33 kDa in strains containing various recombinant plasmids with portions of the McrB region has been reported by Ross et al. (Gene 61:277-289, 1987). The analysis of frameshift and deletion mutants of one such hybrid plasmid, pRAB-13, provided evidence for a second translational initiation site within the McrB open reading frame. The proposed start codon for translation of the 33-kDa peptide lies 481 nucleotides downstream from the initiation codon for the 51-kDa mcrB gene product. The 33-kDa peptide may play a regulatory role in the McrB restriction of DNA containing 5-methylcytosine.

MeSH Terms
Bacterial Proteins/genetics Base Sequence Carrier Proteins/genetics Cloning, Molecular DNA Mutational Analysis DNA Restriction-Modification Enzymes/genetics Escherichia coli/genetics Genes, Bacterial Genetic Complementation Test Methylation Molecular Sequence Data Molecular Weight Restriction Mapping
Chemicals
Bacterial Proteins Carrier Proteins DNA Restriction-Modification Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ross T K
Department of Microbiology, Louisiana State University, Baton Rouge 70803.
Achberger E C
Braymer H D
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.4.1974-1981.1989
Published
1989-04-00
Pages
1974-81
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC209847
Subset
IM
Databases
GENBANK
M24927
External Links
PubMed source DOI: 10.1128/jb.171.4.1974-1981.1989 PMC Full Text View journal details
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