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PMID: 2548920 Published · ppublish English Comparative Study Journal Article

Genetic and physical mapping of the mcrA (rglA) and mcrB (rglB) loci of Escherichia coli K-12.

Genetics ·Vol. 122 ·No. 2 ·1989-06-00 ·Pages 279-96

Raleigh EA, Trimarchi R, Revel H

Abstract

We have genetically analyzed, cloned and physically mapped the modified cytosine-specific restriction determinants mcrA (rglA) and mcrB (rglB) of Escherichia coli K-12. The independently discovered Rgl and Mcr restriction systems are shown to be identical by three criteria: 1) mutants with the RglA- or RglB- phenotypes display the corresponding McrA- or McrB- phenotypes, and vice versa; 2) the gene(s) for RglA and McrA reside together at one locus, while gene(s) for RglB and McrB are coincident at a different locus; and 3) RglA+ and RglB+ recombinant clones complement for the corresponding Mcr-deficient lesions. The mcrA (rglA) gene(s) is on the excisable element e14, just clockwise of purB at 25 min. The mcrB (rglB) gene(s), at 99 min, is in a cluster of restriction functions that includes hsd and mrr, determinants of host-specific restriction (EcoK) and methyladenine-specific restriction respectively. Gene order is mcrB-hsdS-hsdM-hsdR-mrr-serB. Possible models for the acqusition of these restriction determinants by enteric bacteria are discussed.

MeSH Terms
Bacterial Proteins/genetics,metabolism Chromosome Mapping DNA Restriction Enzymes/genetics,metabolism DNA Transposable Elements DNA, Bacterial/genetics,metabolism Escherichia coli/enzymology,genetics Escherichia coli Proteins Genes Genes, Bacterial Models, Genetic Transduction, Genetic
Chemicals
Bacterial Proteins DNA Transposable Elements DNA, Bacterial Escherichia coli Proteins DNA Restriction Enzymes endodeoxyribonuclease McrA mcrB protein, E coli
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Raleigh E A
New England BioLabs, Beverly, Massachusetts 01915-9990.
Trimarchi R
Revel H
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/122.2.279
Published
1989-06-00
Pages
279-96
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1203701
Subset
IM
External Links
PubMed source DOI: 10.1093/genetics/122.2.279 PMC Full Text View journal details
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