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PMID: 1969618 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The human minisatellite consensus at breakpoints of oncogene translocations.

Nucleic acids research ·Vol. 18 ·No. 5 ·1990-03-11 ·Pages 1121-7

Krowczynska AM, Rudders RA, Krontiris TG

Abstract

A reexamination of human minisatellite (hypervariable) regions following the cloning and sequencing of the new minisatellite, VTR1.1, revealed that many of these structures possessed a strongly conserved copy of the chi-like octamer, GC[A/T]GG[A/T]GG. In oncogene translocations apparently created by aberrant VDJ recombinase activity, this VTR octamer was often found within a few bases of the breakpoint (p less than 10(-10)). Three bcl2 rearrangements which occurred within 2 bp of one another were located precisely adjacent to this consensus; it defined the 5' border of that oncogene's major breakpoint cluster. Several c-myc translocations also occurred within 2 bp of this sequence. While the appearance of a chi-like element in polymorphic minisatellite sequences is consistent with a role promoting either recombination or replication slippage, the existence of such elements at sites of somatic translocations suggests chi function in site-specific recombination, perhaps as a subsidiary recognition signal in immunoglobulin gene rearrangement. We discuss the implications of these observations for mechanisms by which oncogene translocations and minisatellite sequences are generated.

MeSH Terms
Base Sequence DNA Nucleotidyltransferases/genetics DNA, Satellite/genetics Gene Rearrangement Humans Immunoglobulin Heavy Chains/genetics Molecular Sequence Data Oncogenes Polymorphism, Restriction Fragment Length Recombination, Genetic Repetitive Sequences, Nucleic Acid Sequence Homology, Nucleic Acid Translocation, Genetic/genetics Tumor Cells, Cultured VDJ Recombinases
Chemicals
DNA, Satellite Immunoglobulin Heavy Chains DNA Nucleotidyltransferases VDJ Recombinases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Krowczynska A M
Department of Medicine (Hematology/Oncology), New England Medical Center Hospitals, Boston, MA 02111.
Rudders R A
Krontiris T G
References (37)
37 references, click to expand
  1. Chi, a promoter of generalized recombination in lambda phage, is present in immunoglobulin genes.
    Nature. 1981 Oct 1;293(5831):402-4 PMID: 6456417
  2. The t(14;18) chromosome translocations involved in B-cell neoplasms result from mistakes in VDJ joining.
    Science. 1985 Sep 27;229(4720):1390-3 PMID: 3929382
  3. Translocation and rearrangements of the c-myc oncogene locus in human undifferentiated B-cell lymphomas.
    Science. 1983 Feb 25;219(4587):963-7 PMID: 6401867
  4. Chi hotspots of generalized recombination.
    Cell. 1983 Oct;34(3):709-10 PMID: 6354468
  5. Identification of reciprocal translocation sites within the c-myc oncogene and immunoglobulin mu locus in a Burkitt lymphoma.
    Nature. 1983 Dec 22-1984 Jan 4;306(5945):799-803 PMID: 6419123
  6. A variant translocation places the lambda immunoglobulin genes 3' to the c-myc oncogene in Burkitt's lymphoma.
    Nature. 1984 Feb 23-29;307(5953):752-5 PMID: 6422306
  7. Activation of the c-myc gene by translocation: a model for translational control.
    Proc Natl Acad Sci U S A. 1983 Dec;80(24):7476-80 PMID: 6324175
  8. Organization of the human myoglobin gene.
    EMBO J. 1984 Feb;3(2):439-46 PMID: 6571704
  9. Clones from the human gene complex coding for salivary proline-rich proteins.
    Proc Natl Acad Sci U S A. 1984 Sep;81(17):5561-5 PMID: 6089212
  10. Activation of a translocated c-myc gene: role of structural alterations in the upstream region.
    Proc Natl Acad Sci U S A. 1984 Nov;81(21):6798-802 PMID: 6593728
  11. Hypervariable 'minisatellite' regions in human DNA.
    Nature. 1985 Mar 7-13;314(6006):67-73 PMID: 3856104
  12. Chromatin structure around the c-myc gene in Burkitt lymphomas with upstream and downstream translocation points.
    Proc Natl Acad Sci U S A. 1985 Apr;82(7):1984-8 PMID: 3856876
  13. Cloning and sequencing of a c-myc oncogene in a Burkitt's lymphoma cell line that is translocated to a germ line alpha switch region.
    Mol Cell Biol. 1985 Mar;5(3):501-9 PMID: 3921823
  14. Clustering of breakpoints on chromosome 11 in human B-cell neoplasms with the t(11;14) chromosome translocation.
    Nature. 1985 May 23-29;315(6017):340-3 PMID: 3923362
  15. Structural analysis of both products of a reciprocal translocation between c-myc and immunoglobulin loci in Burkitt lymphoma.
    Nucleic Acids Res. 1985 Mar 25;13(6):2141-52 PMID: 3923436
  16. Nucleotide sequence of a t(14;18) chromosomal breakpoint in follicular lymphoma and demonstration of a breakpoint-cluster region near a transcriptionally active locus on chromosome 18.
    Proc Natl Acad Sci U S A. 1985 Nov;82(21):7439-43 PMID: 2865728
  17. Regulation of the assembly and expression of variable-region genes.
    Annu Rev Immunol. 1986;4:339-68 PMID: 3085692
  18. Structure and evolution of the human involucrin gene.
    Cell. 1986 Aug 15;46(4):583-9 PMID: 2873896
  19. Cloning and structural analysis of cDNAs for bcl-2 and a hybrid bcl-2/immunoglobulin transcript resulting from the t(14;18) translocation.
    Cell. 1986 Oct 10;47(1):19-28 PMID: 2875799
  20. A variable tandem repeat locus mapped to chromosome band 10q26 is amplified and rearranged in leukocyte DNAs of two cancer patients.
    Nucleic Acids Res. 1986 Oct 24;14(20):7929-37 PMID: 3022240
  21. Mouse DNA 'fingerprints': analysis of chromosome localization and germ-line stability of hypervariable loci in recombinant inbred strains.
    Nucleic Acids Res. 1987 Apr 10;15(7):2823-36 PMID: 3562240
  22. Mechanism of the t(14;18) chromosomal translocation: structural analysis of both derivative 14 and 18 reciprocal partners.
    Proc Natl Acad Sci U S A. 1987 Apr;84(8):2396-400 PMID: 3104914
  23. Demographic study of a wild house sparrow population by DNA fingerprinting.
    Nature. 1987 May 14-20;327(6118):147-9 PMID: 3574474
  24. The role of chromosomal translocations in B- and T-cell neoplasia.
    Annu Rev Immunol. 1987;5:253-77 PMID: 2885014
  25. The t(8;14) chromosome translocation of the Burkitt lymphoma cell line Daudi occurred during immunoglobulin gene rearrangement and involved the heavy chain diversity region.
    Proc Natl Acad Sci U S A. 1987 Oct;84(19):6835-9 PMID: 3116544
  26. Prediction of the risk of hereditary retinoblastoma, using DNA polymorphisms within the retinoblastoma gene.
    N Engl J Med. 1988 Jan 21;318(3):151-7 PMID: 2892131
  27. Nucleotide sequence of a mouse minisatellite DNA.
    Nucleic Acids Res. 1988 Feb 11;16(3):1197 PMID: 3344205
  28. The t(8;14) breakpoint of the EW 36 undifferentiated lymphoma cell line lies 5' of MYC in a region prone to involvement in endemic Burkitt's lymphomas.
    Nucleic Acids Res. 1988 Mar 25;16(5):2077-85 PMID: 3128772
  29. DNA fingerprinting in domestic animals using four different minisatellite probes.
    Cytogenet Cell Genet. 1988;47(3):127-31 PMID: 3163967
  30. A cluster of chromosome 11p13 translocations found via distinct D-D and D-D-J rearrangements of the human T cell receptor delta chain gene.
    EMBO J. 1988 Jul;7(7):2011-7 PMID: 3416831
  31. Evolution of B-cell malignancy: pre-B-cell leukemia resulting from MYC activation in a B-cell neoplasm with a rearranged BCL2 gene.
    Proc Natl Acad Sci U S A. 1988 Nov;85(22):8548-52 PMID: 3186743
  32. A new DNA binding and dimerization motif in immunoglobulin enhancer binding, daughterless, MyoD, and myc proteins.
    Cell. 1989 Mar 10;56(5):777-83 PMID: 2493990
  33. High-resolution analysis of a hypervariable region in the human apolipoprotein B gene.
    Am J Hum Genet. 1989 Sep;45(3):458-64 PMID: 2773938
  34. Conservation of an immunoglobulin variable-region gene family indicates a specific, noncoding function.
    Proc Natl Acad Sci U S A. 1989 Oct;86(19):7460-4 PMID: 2798416
  35. Allele-specific deletion in exon I of the HRAS1 gene.
    Am J Hum Genet. 1989 Nov;45(5):689-96 PMID: 2573274
  36. Characterization of a highly polymorphic region 5' to JH in the human immunoglobulin heavy chain.
    Nucleic Acids Res. 1987 May 11;15(9):3845-57 PMID: 2884636
  37. Structure of human immunoglobulin gamma genes: implications for evolution of a gene family.
    Cell. 1982 Jun;29(2):671-9 PMID: 6811139
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/18.5.1121
Published
1990-03-11
Pages
1121-7
Language
English
Region
England
NLM ID
0411011
PMCID
PMC330424
Subset
IM
Grants
NCI NIH HHS · CA40725 · United States
NCI NIH HHS · CA45052 · United States
External Links
PubMed source DOI: 10.1093/nar/18.5.1121 PMC Full Text View journal details
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