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PMID: 1971261 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Direct evidence that the FimH protein is the mannose-specific adhesin of Escherichia coli type 1 fimbriae.

Infection and immunity ·Vol. 58 ·No. 6 ·1990-06-00 ·Pages 1995-8

Krogfelt KA, Bergmans H, Klemm P

Abstract

Type 1 fimbriae of Escherichia coli are surface organelles which mediate binding to D-mannose-containing structures. By direct binding of FimH to D-mannose attached to a carrier protein, we demonstrated that this protein was uniquely responsible for the receptor specificity. Furthermore, we show by receptor immunoelectron microscopy that the FimH protein is located laterally in the structure of the type 1 fimbriae.

MeSH Terms
Adhesins, Escherichia coli Bacterial Adhesion Bacterial Outer Membrane Proteins/genetics,metabolism Bacterial Proteins/genetics,metabolism Cloning, Molecular Escherichia coli/genetics,metabolism Fimbriae Proteins Fimbriae, Bacterial/metabolism Genes, Bacterial Mannose/metabolism Microscopy, Electron Molecular Weight Plasmids Recombinant Proteins
Chemicals
Adhesins, Escherichia coli Bacterial Outer Membrane Proteins Bacterial Proteins Recombinant Proteins fimH protein, E coli Fimbriae Proteins Mannose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Krogfelt K A
Department of Microbiology, Technical University of Denmark, Lyngby.
Bergmans H
Klemm P
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24 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.58.6.1995-1998.1990
Published
1990-06-00
Pages
1995-8
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC258756
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.58.6.1995-1998.1990 PMC Full Text View journal details
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