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PMID: 1972545 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Molecular cloning of the yeast mitochondrial aconitase gene (ACO1) and evidence of a synergistic regulation of expression by glucose plus glutamate.

Molecular and cellular biology ·Vol. 10 ·No. 7 ·1990-07-00 ·Pages 3551-61

Gangloff SP, Marguet D, Lauquin GJ

Abstract

We have isolated genomic clones complementing the aconitase-deficient strain (glu1-1) of Saccharomyces cerevisiae. Identification of the aconitase gene was established by enzymatic assays and molecular analyses. The corresponding mRNA has been characterized, and its direction of transcription has been determined. The complete nucleotide sequence revealed strong amino acid homologies with the sequences of some peptides isolated from the mammalian protein. Disruption of the gene by deletion-insertion led to glutamate auxotrophy. Expression of the aconitase gene was sensitive to glucose repression and was synergistically down regulated by glucose and glutamate.

MeSH Terms
Aconitate Hydratase/genetics,metabolism Amino Acid Sequence Base Sequence Cloning, Molecular DNA, Fungal/genetics DNA, Mitochondrial/genetics Escherichia coli/genetics Gene Expression Regulation, Fungal/drug effects Genes, Fungal Genetic Vectors Glucose/pharmacology Glutamates/pharmacology Glutamic Acid Mitochondria/enzymology Molecular Sequence Data Recombinant Proteins/metabolism Restriction Mapping Saccharomyces cerevisiae/enzymology,genetics,growth & development Sequence Homology, Nucleic Acid Transcription, Genetic
Chemicals
DNA, Fungal DNA, Mitochondrial Glutamates Recombinant Proteins Glutamic Acid Aconitate Hydratase Glucose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gangloff S P
Institut de Biochimie Cellulaire et Neurochimie, Centre National de la Recherche Scientifique, Bordeaux, France.
Marguet D
Lauquin G J
References (48)
48 references, click to expand
  1. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  2. Effect of different nutritional conditions on the synthesis of tricarboxylic acid cycle enzymes.
    J Bacteriol. 1967 Jun;93(6):1777-87 PMID: 4960893
  3. Changes in the enzyme activities of Saccharomyces cerevisiae during aerobic growth on different carbon sources.
    Biochem J. 1965 Oct;97(1):284-97 PMID: 16749116
  4. N2,N2-dimethylguanosine-specific tRNA methyltransferase contains both nuclear and mitochondrial targeting signals in Saccharomyces cerevisiae.
    J Cell Biol. 1989 Oct;109(4 Pt 1):1411-9 PMID: 2677019
  5. The codon Adaptation Index--a measure of directional synonymous codon usage bias, and its potential applications.
    Nucleic Acids Res. 1987 Feb 11;15(3):1281-95 PMID: 3547335
  6. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  7. Comparative biosequence metrics.
    J Mol Evol. 1981;18(1):38-46 PMID: 7334527
  8. The soluble "high potential" type iron-sulfur protein from mitochondria is aconitase.
    J Biol Chem. 1978 Apr 25;253(8):2514-7 PMID: 204652
  9. Identification of the reactive sulfhydryl and sequences of cysteinyl-tryptic peptides from beef heart aconitase.
    J Biol Chem. 1988 Jun 15;263(17):8184-9 PMID: 3372519
  10. Yeast HAP2 and HAP3 activators both bind to the CYC1 upstream activation site, UAS2, in an interdependent manner.
    Cell. 1987 Dec 24;51(6):953-61 PMID: 2826015
  11. The active site sulfhydryl of aconitase is not required for catalytic activity.
    J Biol Chem. 1988 Jun 15;263(17):8190-3 PMID: 2836416
  12. The synthesis of yeast matrix mitochondrial enzymes is regulated by different levels of mitochondrial function.
    Arch Biochem Biophys. 1977 Dec;184(2):355-63 PMID: 339842
  13. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.
    Anal Biochem. 1984 Feb;137(1):266-7 PMID: 6329026
  14. Mössbauer and EPR studies of activated aconitase: development of a localized valence state at a subsite of the [4Fe-4S] cluster on binding of citrate.
    Proc Natl Acad Sci U S A. 1983 Aug;80(15):4674-8 PMID: 6308639
  15. Cysteine labeling studies of beef heart aconitase containing a 4Fe, a cubane 3Fe, or a linear 3Fe cluster.
    J Biol Chem. 1989 Dec 5;264(34):20385-93 PMID: 2511202
  16. Mutational analysis of upstream activation sequence 2 of the CYC1 gene of Saccharomyces cerevisiae: a HAP2-HAP3-responsive site.
    Mol Cell Biol. 1988 Feb;8(2):647-54 PMID: 2832731
  17. DNA sequence and transcript mapping of MOD5: features of the 5' region which suggest two translational starts.
    Mol Cell Biol. 1987 Jan;7(1):185-91 PMID: 3031457
  18. The HTS1 gene encodes both the cytoplasmic and mitochondrial histidine tRNA synthetases of S. cerevisiae.
    Cell. 1986 Jul 18;46(2):235-43 PMID: 3521891
  19. Studies on aconitase species from Saccharomyces cerevisiae, porcine and bovine heart, obtained by a modified isolation method.
    Biochim Biophys Acta. 1983 Aug 16;746(3):133-7 PMID: 6349693
  20. The mechanism of aconitase action. I. Preparation, physical properties of the enzyme, and activation by iron (II).
    J Biol Chem. 1971 Feb 10;246(3):772-9 PMID: 5542689
  21. Studies on the regulation and localization of the glyoxylate cycle enzymes in Saccharomyces cerevisiae.
    Eur J Biochem. 1969 Aug;10(1):83-9 PMID: 5345986
  22. New M13 vectors for cloning.
    Methods Enzymol. 1983;101:20-78 PMID: 6310323
  23. Mitochondrial and cytoplasmic fumarases in Saccharomyces cerevisiae are encoded by a single nuclear gene FUM1.
    J Biol Chem. 1987 Sep 5;262(25):12275-82 PMID: 3040736
  24. Transcriptional regulation of the yeast cytochrome c gene.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3627-31 PMID: 226972
  25. Determination of serum proteins by means of the biuret reaction.
    J Biol Chem. 1949 Feb;177(2):751-66 PMID: 18110453
  26. Amino acid sequence of a peptide containing an essential cysteine residue of pig heart aconitase.
    Biochim Biophys Acta. 1981 Feb 27;667(2):457-61 PMID: 7213810
  27. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  28. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  29. The making of strand-specific M13 probes.
    Gene. 1982 Mar;17(3):271-7 PMID: 7049837
  30. One-step gene disruption in yeast.
    Methods Enzymol. 1983;101:202-11 PMID: 6310324
  31. Transformation of yeast by a replicating hybrid plasmid.
    Nature. 1978 Sep 14;275(5676):104-9 PMID: 357984
  32. The yeast VAS1 gene encodes both mitochondrial and cytoplasmic valyl-tRNA synthetases.
    J Biol Chem. 1988 Jan 5;263(1):52-7 PMID: 3275649
  33. Two differentially regulated mRNAs with different 5' ends encode secreted with intracellular forms of yeast invertase.
    Cell. 1982 Jan;28(1):145-54 PMID: 7039847
  34. Mechanism of aconitase action. I. The hydrogen transfer reaction.
    J Biol Chem. 1967 Apr 25;242(8):1870-9 PMID: 6024777
  35. Bacillus subtilis citB gene is regulated synergistically by glucose and glutamine.
    J Bacteriol. 1985 Oct;164(1):155-64 PMID: 2413006
  36. On pig heart aconitase.
    Biochem Biophys Res Commun. 1972 May 26;47(4):740-5 PMID: 5026292
  37. Mitochondrial and nonmitochondrial citrate synthases in Saccharomyces cerevisiae are encoded by distinct homologous genes.
    Mol Cell Biol. 1986 Dec;6(12):4509-15 PMID: 3540614
  38. Mitochondrial targeting sequences may form amphiphilic helices.
    EMBO J. 1986 Jun;5(6):1335-42 PMID: 3015599
  39. Cloning and structural characterization of porcine heart aconitase.
    J Biol Chem. 1990 Feb 15;265(5):2814-21 PMID: 2303429
  40. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  41. Iron-sulphur clusters: agents of electron transfer and storage, and direct participants in enzymic reactions. Tenth Keilin memorial lecture.
    Biochem Soc Trans. 1986 Jun;14(3):527-33 PMID: 3732590
  42. Saccharomyces cerevisiae contains two functional citrate synthase genes.
    Mol Cell Biol. 1986 Jun;6(6):1936-42 PMID: 3023912
  43. Ethidium bromide induced mutation of yeast mitochondria: complete transformation of cells into respiratory deficient non-chromosomal "petites".
    Biochem Biophys Res Commun. 1968 Feb 15;30(3):232-9 PMID: 5647224
  44. Isolation and expression of the gene encoding yeast mitochondrial malate dehydrogenase.
    J Bacteriol. 1987 Nov;169(11):5157-66 PMID: 3312168
  45. Structure of activated aconitase: formation of the [4Fe-4S] cluster in the crystal.
    Proc Natl Acad Sci U S A. 1989 May;86(10):3639-43 PMID: 2726740
  46. Extramitochondrial citrate synthase activity in bakers' yeast.
    Mol Cell Biol. 1986 Feb;6(2):488-93 PMID: 3023850
  47. Glutamate auxotrophs in Saccharomyces 1. I. The biochemical lesion in the glt-1 mutants-2.
    Biochem Biophys Res Commun. 1964;14:193-7 PMID: 5836504
  48. Transformation of intact yeast cells treated with alkali cations.
    J Bacteriol. 1983 Jan;153(1):163-8 PMID: 6336730
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.7.3551-3561.1990
Published
1990-07-00
Pages
3551-61
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360790
Subset
IM
Databases
GENBANK
M33131
External Links
PubMed source DOI: 10.1128/mcb.10.7.3551-3561.1990 PMC Full Text View journal details
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