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PMID: 3312168 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation and expression of the gene encoding yeast mitochondrial malate dehydrogenase.

Journal of bacteriology ·Vol. 169 ·No. 11 ·1987-11-00 ·Pages 5157-66

McAlister-Henn L, Thompson LM

Abstract

The mitochondrial tricarboxylic acid cycle enzyme malate dehydrogenase was purified from Saccharomyces cerevisiae, and an antibody to the purified enzyme was obtained in rabbits. Immunoscreening of a yeast genomic DNA library cloned into a lambda gt11 expression vector with anti-malate dehydrogenase immunoglobulin G resulted in identification of a lambda recombinant encoding an immunoreactive beta-galactosidase fusion protein. The yeast DNA portion of the coding region for the fusion protein translates into an amino acid sequence which is very similar to carboxy-terminal sequences of malate dehydrogenases from other organisms. In s. cerevisiae transformed with a multicopy plasmid carrying the complete malate dehydrogenase gene, the specific activity and immunoreactivity of the mitochondrial isozyme are increased by eightfold. Expression of both the chromosomal and plasmid-borne genes is repressed by growth on glucose. Disruption of the chromosomal malate dehydrogenase gene in haploid S. cerevisiae produces mutants unable to grow on acetate and impaired in growth on glycerol plus lactate as carbon sources.

MeSH Terms
Amino Acid Sequence Animals Escherichia coli/enzymology,genetics Genes Genes, Fungal Isocitrate Dehydrogenase/genetics Malate Dehydrogenase/genetics,isolation & purification Mitochondria/enzymology Molecular Sequence Data Saccharomyces cerevisiae/enzymology,genetics Swine Transcription, Genetic
Chemicals
Malate Dehydrogenase Isocitrate Dehydrogenase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
McAlister-Henn L
Department of Biological Chemistry, California College of Medicine, University of California, Irvine 92717.
Thompson L M
References (36)
36 references, click to expand
  1. Regulation of mitochondrial malate dehydrogenase. Evidence for an allosteric citrate-binding site.
    J Biol Chem. 1982 Nov 25;257(22):13233-9 PMID: 7142142
  2. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  3. Transformation of intact yeast cells treated with alkali cations.
    J Bacteriol. 1983 Jan;153(1):163-8 PMID: 6336730
  4. The presence of a histidine-aspartic acid pair in the active site of 2-hydroxyacid dehydrogenases. X-ray refinement of cytoplasmic malate dehydrogenase.
    J Biol Chem. 1983 Jan 10;258(1):472-82 PMID: 6848515
  5. New M13 vectors for cloning.
    Methods Enzymol. 1983;101:20-78 PMID: 6310323
  6. One-step gene disruption in yeast.
    Methods Enzymol. 1983;101:202-11 PMID: 6310324
  7. Yeast RNA polymerase II genes: isolation with antibody probes.
    Science. 1983 Nov 18;222(4625):778-82 PMID: 6356359
  8. Distinctly regulated tandem upstream activation sites mediate catabolite repression of the CYC1 gene of S. cerevisiae.
    Cell. 1984 Feb;36(2):503-11 PMID: 6319028
  9. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.
    Anal Biochem. 1984 Feb;137(1):266-7 PMID: 6329026
  10. Isolation and expression of the Escherichia coli gene encoding malate dehydrogenase.
    J Bacteriol. 1985 Sep;163(3):1074-9 PMID: 2993232
  11. Substrate channeling of oxalacetate in solid-state complexes of malate dehydrogenase and citrate synthase.
    J Biol Chem. 1985 Dec 5;260(28):15008-12 PMID: 4066662
  12. Isolation and characterization of yeast strains carrying mutations in the glyceraldehyde-3-phosphate dehydrogenase genes.
    J Biol Chem. 1985 Dec 5;260(28):15013-8 PMID: 2999100
  13. The three-dimensional structure of porcine heart mitochondrial malate dehydrogenase at 3.0-A resolution.
    J Biol Chem. 1986 Jul 15;261(20):9461-4 PMID: 3722205
  14. Genetics of mitochondrial biogenesis.
    Annu Rev Biochem. 1986;55:249-85 PMID: 2427014
  15. Mutants of yeast with altered oxidative energy metabolism: selection and genetic characterization.
    J Bacteriol. 1969 Nov;100(2):647-57 PMID: 5354937
  16. Identification and isolation of ovalbumin-synthesizing polysomes. I. Specific binding of 125 I-anti-ovalbumin to polysomes.
    J Biol Chem. 1972 Apr 25;247(8):2316-21 PMID: 4111967
  17. Assembly of the mitochondrial membrane system: isolation of nuclear and cytoplasmic mutants of Saccharomyces cerevisiae with specific defects in mitochondrial functions.
    J Bacteriol. 1975 Jun;122(3):826-31 PMID: 168180
  18. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  19. Isolation and characterization of yeast mutants defective in intermediary carbon metabolism and in carbon catabolite derepression.
    Mol Gen Genet. 1977 Jul 20;154(2):213-20 PMID: 197391
  20. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  21. The malate dehydrogenase isoenzymes of Saccharomyces cerevisiae. Purification, characterisation and studies on their regulation.
    Eur J Biochem. 1978 Feb 1;83(1):67-76 PMID: 342240
  22. Glycolysis mutants in Saccharomyces cerevisiae.
    Genetics. 1978 Jan;88(1):1-11 PMID: 147195
  23. Transformation of yeast.
    Proc Natl Acad Sci U S A. 1978 Apr;75(4):1929-33 PMID: 347451
  24. The isolation of structural genes from libraries of eucaryotic DNA.
    Cell. 1978 Oct;15(2):687-701 PMID: 719759
  25. Analysis of products of mitochondrial protein synthesis in yeast: genetic and biochemical aspects.
    Methods Enzymol. 1979;56:58-66 PMID: 379518
  26. Filter replicas and permanent collections of recombinant DNA plasmids.
    Nucleic Acids Res. 1979 Dec 20;7(8):2115-36 PMID: 118435
  27. The structure of transposable yeast mating type loci.
    Cell. 1980 Mar;19(3):753-64 PMID: 6244896
  28. Alterations in translatable ribonucleic acid after heat shock of Saccharomyces cerevisiae.
    J Bacteriol. 1980 Aug;143(2):603-12 PMID: 7009554
  29. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.
    Anal Biochem. 1981 Apr;112(2):195-203 PMID: 6266278
  30. Demonstration of physical interactions between consecutive enzymes of the citric acid cycle and of the aspartate-malate shuttle. A study involving fumarase, malate dehydrogenase, citrate synthesis and aspartate aminotransferase.
    Eur J Biochem. 1981 Jul;117(3):527-35 PMID: 7285903
  31. Targeted deletion of a yeast enolase structural gene. Identification and isolation of yeast enolase isozymes.
    J Biol Chem. 1982 Jun 25;257(12):7181-8 PMID: 6282834
  32. Import of proteins into mitochondria. Cytochrome b2 and cytochrome c peroxidase are located in the intermembrane space of yeast mitochondria.
    J Biol Chem. 1982 Nov 10;257(21):13028-33 PMID: 6290489
  33. Isolation and nucleotide sequence of a cDNA clone encoding rat mitochondrial malate dehydrogenase.
    Nucleic Acids Res. 1986 Aug 11;14(15):6053-66 PMID: 3755817
  34. Saccharomyces cerevisiae contains two functional citrate synthase genes.
    Mol Cell Biol. 1986 Jun;6(6):1936-42 PMID: 3023912
  35. Mitochondrial and nonmitochondrial citrate synthases in Saccharomyces cerevisiae are encoded by distinct homologous genes.
    Mol Cell Biol. 1986 Dec;6(12):4509-15 PMID: 3540614
  36. Amino acid sequence homology among the 2-hydroxy acid dehydrogenases: mitochondrial and cytoplasmic malate dehydrogenases form a homologous system with lactate dehydrogenase.
    Proc Natl Acad Sci U S A. 1982 Oct;79(20):6166-70 PMID: 6959107
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.11.5157-5166.1987
Published
1987-11-00
Pages
5157-66
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC213921
Subset
IM
Grants
NIGMS NIH HHS · GM 33218 · United States
External Links
PubMed source DOI: 10.1128/jb.169.11.5157-5166.1987 PMC Full Text View journal details
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