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PMID: 1990272 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

The DAL81 gene product is required for induced expression of two differently regulated nitrogen catabolic genes in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 11 ·No. 2 ·1991-02-00 ·Pages 1161-6

Bricmont PA, Daugherty JR, Cooper TG

Abstract

We demonstrate that the DAL81 gene, previously thought to be specifically required for induced expression of the allantoin pathway genes in Saccharomyces cerevisiae, functions in a more global manner. The data presented show it to be required for utilization of 4-aminobutyrate as a nitrogen source and for 4-aminobutyrate-induced increases in the steady-state levels of UGA1 mRNA. The DAL81 gene encodes a 970-amino-acid protein containing sequences homologous to the Zn(II)2Cys6 motif and two stretches of polyglutamine residues. Deletion of sequences homologous to the Zn(II)2Cys6 motif did not result in a detectable loss of function. On the other hand, loss of one of the polyglutamine stretches, but not the other, resulted in a 50% loss of DAL81 function.

Related Genes
UGA1 dal81
MeSH Terms
Amino Acid Sequence Base Sequence Chromosome Deletion Fungal Proteins/genetics Gene Expression Regulation, Fungal Genes, Fungal Molecular Sequence Data Nitrogen/metabolism Phenotype Plasmids RNA, Messenger/genetics Restriction Mapping Saccharomyces cerevisiae/enzymology,genetics Saccharomyces cerevisiae Proteins Sequence Homology, Nucleic Acid Transcription Factors
Chemicals
DAL81 protein, S cerevisiae Fungal Proteins RNA, Messenger Saccharomyces cerevisiae Proteins Transcription Factors Nitrogen
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bricmont P A
Department of Microbiology and Immunology, University of Tennessee, Memphis 38163.
Daugherty J R
Cooper T G
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31 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.2.1161-1166.1991
Published
1991-02-00
Pages
1161-6
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359801
Subset
IM
Grants
NIGMS NIH HHS · R01 GM035642 · United States
NIGMS NIH HHS · GM-20693 · United States
NIGMS NIH HHS · GM-35642 · United States
Databases
GENBANK
M35697, M35698, M35699, M35700, M59928, M59929, M60415, M73693, M73694, M73695
External Links
PubMed source DOI: 10.1128/mcb.11.2.1161-1166.1991 PMC Full Text View journal details
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