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PMID: 2674683 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A gene product needed for induction of allantoin system genes in Saccharomyces cerevisiae but not for their transcriptional activation.

Molecular and cellular biology ·Vol. 9 ·No. 9 ·1989-09-00 ·Pages 3869-77

Bricmont PA, Cooper TG

Abstract

The allantoin-degradative pathway of Saccharomyces cerevisiae consists of several genes whose expression is highly induced by the presence of allophanic acid. Induced expression requires a functional DAL81 gene product. Analysis of these genes has demonstrated the presence of three cis-acting elements in the upstream regions: (i) an upstream activation sequence (UAS) required for transcriptional activation in an inducer-independent fashion, (ii) an upstream repression sequence (URS) that mediates inhibition of this transcriptional activation, and (iii) an upstream induction sequence (UIS) needed for a response to inducer. The UIS element mediates inhibition of URS-mediated function when inducer is present. We cloned and characterized the DAL81 gene and identified the element with which it was associated. The gene was found to encode a rare 3.2-kilobase-pair mRNA. The amount of DAL81-specific RNA responded neither to induction nor to nitrogen catabolite repression. Deletion of the DAL81 gene resulted in loss of induction but did not significantly affect basal level expression of the DAL7 and DUR1,2 genes or the UAS and URS functions present in plasmid constructions. These data suggest that (i) transcriptional activation of the DAL genes and their responses to inducer are mediated by different factors and cis-acting sequences and (ii) the UIS functions only when a wild-type DAL81 gene product is available.

MeSH Terms
Allantoin/metabolism Alleles Chromosome Deletion DNA, Fungal/genetics Gene Expression Regulation Genes, Fungal Genetic Complementation Test Mutation Phenotype Plasmids RNA, Fungal/genetics RNA, Messenger/genetics Saccharomyces cerevisiae/genetics,metabolism Transcription, Genetic
Chemicals
DNA, Fungal RNA, Fungal RNA, Messenger Allantoin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bricmont P A
Department of Microbiology and Immunology, University of Tennessee, Memphis 38163.
Cooper T G
References (43)
43 references, click to expand
  1. Identification of sequences responsible for transcriptional activation of the allantoate permease gene in Saccharomyces cerevisiae.
    Mol Cell Biol. 1989 Feb;9(2):602-8 PMID: 2651902
  2. Isolation of a yeast centromere and construction of functional small circular chromosomes.
    Nature. 1980 Oct 9;287(5782):504-9 PMID: 6999364
  3. High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.
    Proc Natl Acad Sci U S A. 1979 Mar;76(3):1035-9 PMID: 375221
  4. The induction of urea carboxylase and allophanate hydrolase in Saccharomyces cerevisiae.
    J Biol Chem. 1973 Sep 10;248(17):6203-9 PMID: 4580053
  5. Functional domains of the yeast regulatory protein GAL4.
    Proc Natl Acad Sci U S A. 1986 Sep;83(17):6553-7 PMID: 2944111
  6. Genetic control of galactokinase synthesis in Saccharomyces cerevisiae: evidence for constitutive expression of the positive regulatory gene gal4.
    J Bacteriol. 1978 May;134(2):446-57 PMID: 207666
  7. Isolation and characterization of mutants that produce the allantoin-degrading enzymes constitutively in Saccharomyces cerevisiae.
    Mol Cell Biol. 1982 Sep;2(9):1088-95 PMID: 6757722
  8. Identification of the ureidoglycolate hydrolase gene in the DAL gene cluster of Saccharomyces cerevisiae.
    Mol Cell Biol. 1985 Sep;5(9):2279-88 PMID: 3915539
  9. Nitrogen repression of the allantoin degradative enzymes in Saccharomyces cerevisiae.
    J Bacteriol. 1974 Jun;118(3):821-9 PMID: 4598006
  10. Induction and repression of the urea amidolyase gene in Saccharomyces cerevisiae.
    Mol Cell Biol. 1986 Nov;6(11):3954-64 PMID: 3025621
  11. Uninducible mutants in the gal i locus of Saccharomyces cerevisiae.
    J Bacteriol. 1972 Mar;109(3):1139-43 PMID: 4551746
  12. Transcriptional regulation of the DAL5 gene in Saccharomyces cerevisiae.
    J Bacteriol. 1987 Aug;169(8):3521-4 PMID: 3301804
  13. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  14. Deletion analysis of GAL4 defines two transcriptional activating segments.
    Cell. 1987 Mar 13;48(5):847-53 PMID: 3028647
  15. Regulation of genes controlling synthesis of the galactose pathway enzymes in yeast.
    Genetics. 1966 Sep;54(3):911-6 PMID: 5970626
  16. A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains.
    Genetics. 1987 Aug;116(4):541-5 PMID: 3305158
  17. Cloning of yeast gene for trichodermin resistance and ribosomal protein L3.
    Proc Natl Acad Sci U S A. 1981 Jan;78(1):238-42 PMID: 7017711
  18. The DAL7 promoter consists of multiple elements that cooperatively mediate regulation of the gene's expression.
    Mol Cell Biol. 1989 Aug;9(8):3231-43 PMID: 2552287
  19. One-step gene disruption in yeast.
    Methods Enzymol. 1983;101:202-11 PMID: 6310324
  20. Two differentially regulated mRNAs with different 5' ends encode secreted with intracellular forms of yeast invertase.
    Cell. 1982 Jan;28(1):145-54 PMID: 7039847
  21. Biochemical Mutants in the Smut Fungus Ustilago Maydis.
    Genetics. 1949 Sep;34(5):607-26 PMID: 17247336
  22. Oxaluric acid: a non-metabolizable inducer of the allantoin degradative enzymes in Saccharomyces cerevisiae.
    J Bacteriol. 1974 Mar;117(3):1240-7 PMID: 4591950
  23. Transformation of yeast.
    Proc Natl Acad Sci U S A. 1978 Apr;75(4):1929-33 PMID: 347451
  24. Interaction of GAL4 and GAL80 gene regulatory proteins in vitro.
    Mol Cell Biol. 1987 Oct;7(10):3446-51 PMID: 3316976
  25. ENZYMATIC EXPRESSION AND GENETIC LINKAGE OF GENES CONTROLLING GALACTOSE UTILIZATION IN SACCHAROMYCES.
    Genetics. 1964 May;49:837-44 PMID: 14158615
  26. A Critical Evaluation of the Nitrogen Assimilation Tests Commonly Used in the Classification of Yeasts.
    J Bacteriol. 1946 Sep;52(3):293-301 PMID: 16561177
  27. Induction of the allantoin degradative enzymes in Saccharomyces cerevisiae by the last intermediate of the pathway.
    Proc Natl Acad Sci U S A. 1973 Aug;70(8):2340-4 PMID: 4599622
  28. Mutations that inactivate a yeast transcriptional regulatory protein cluster in an evolutionarily conserved DNA binding domain.
    Proc Natl Acad Sci U S A. 1987 Apr;84(8):2401-5 PMID: 3550810
  29. Constitutive synthesis of the GAL4 protein, a galactose pathway regulator in Saccharomyces cerevisiae.
    Cell. 1979 Jan;16(1):89-95 PMID: 369708
  30. Pleiotropic control of five eucaryotic genes by multiple regulatory elements.
    J Bacteriol. 1982 Sep;151(3):1237-46 PMID: 7050082
  31. The actin genes of Drosophila: a dispersed multigene family.
    Cell. 1980 Feb;19(2):365-78 PMID: 6244106
  32. Regulation of the galactose pathway in Saccharomyces cerevisiae: induction of uridyl transferase mRNA and dependency on GAL4 gene function.
    Proc Natl Acad Sci U S A. 1978 Jun;75(6):2878-82 PMID: 351620
  33. The carboxy-terminal 30 amino acids of GAL4 are recognized by GAL80.
    Cell. 1987 Jul 3;50(1):137-42 PMID: 3297349
  34. Isolation of the CAR1 gene from Saccharomyces cerevisiae and analysis of its expression.
    Mol Cell Biol. 1982 Dec;2(12):1514-23 PMID: 14582193
  35. A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance.
    Mol Gen Genet. 1984;197(2):345-6 PMID: 6394957
  36. Isolation of the yeast regulatory gene GAL4 and analysis of its dosage effects on the galactose/melibiose regulon.
    Proc Natl Acad Sci U S A. 1982 Nov;79(22):6971-5 PMID: 6294669
  37. Interaction of positive and negative regulatory proteins in the galactose regulon of yeast.
    Cell. 1987 Jul 3;50(1):143-6 PMID: 3297350
  38. Specific DNA binding of GAL4, a positive regulatory protein of yeast.
    Cell. 1985 Apr;40(4):767-74 PMID: 3886158
  39. Location of the genes that control induction of the allantoin-degrading enzymes in Saccharomyces cerevisiae.
    Genetics. 1984 Dec;108(4):827-31 PMID: 6392015
  40. A physical, genetic and transcriptional map of the cloned his3 gene region of Saccharomyces cerevisiae.
    J Mol Biol. 1980 Jan 25;136(3):309-32 PMID: 6246242
  41. Disruption of regulatory gene GAL80 in Saccharomyces cerevisiae: effects on carbon-controlled regulation of the galactose/melibiose pathway genes.
    Mol Cell Biol. 1984 Aug;4(8):1521-7 PMID: 6092916
  42. Function of positive regulatory gene gal4 in the synthesis of galactose pathway enzymes in Saccharomyces cerevisiae: evidence that the GAL81 region codes for part of the gal4 protein.
    J Bacteriol. 1980 Feb;141(2):508-27 PMID: 6988385
  43. Transformation of intact yeast cells treated with alkali cations.
    J Bacteriol. 1983 Jan;153(1):163-8 PMID: 6336730
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.9.3869-3877.1989
Published
1989-09-00
Pages
3869-77
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362448
Subset
IM
Grants
NIGMS NIH HHS · R01 GM035642 · United States
NIGMS NIH HHS · GM-19386 · United States
NIGMS NIH HHS · GM-35536 · United States
NIGMS NIH HHS · GM-35642 · United States
External Links
PubMed source DOI: 10.1128/mcb.9.9.3869-3877.1989 PMC Full Text View journal details
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