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PMID: 3316976 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Interaction of GAL4 and GAL80 gene regulatory proteins in vitro.

Molecular and cellular biology ·Vol. 7 ·No. 10 ·1987-10-00 ·Pages 3446-51

Lue NF, Chasman DI, Buchman AR, Kornberg RD

Abstract

The GAL80 protein of Saccharomyces cerevisiae, synthesized in vitro, bound tightly to GAL4 protein and to a GAL4 protein-upstream activation sequence DNA complex, as shown by (i) coimmunoprecipitation of GAL4 and GAL80 proteins with anti-GAL4 antiserum, (ii) an electrophoretic mobility shift of a GAL4 protein-upstream activation sequence DNA complex upon the addition of GAL80 protein, and (iii) GAL4-dependent binding of GAL80 protein to upstream activation sequence DNA immobilized on Sepharose beads. Anti-GAL4 antisera were raised against a GAL4-URA3 fusion protein, which could be purified to homogeneity in a single step with the use of an affinity chromatographic procedure for the URA3 gene product.

MeSH Terms
DNA, Fungal/genetics DNA-Binding Proteins/physiology Fungal Proteins/physiology Galactose/physiology Gene Expression Regulation Immunologic Techniques Macromolecular Substances Protein Binding Recombinant Fusion Proteins/immunology Saccharomyces cerevisiae/genetics Transcription Factors/physiology
Chemicals
DNA, Fungal DNA-Binding Proteins Fungal Proteins Macromolecular Substances Recombinant Fusion Proteins Transcription Factors Galactose
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lue N F
Department of Cell Biology, Stanford University School of Medicine, California 94305.
Chasman D I
Buchman A R
Kornberg R D
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34 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.10.3446-3451.1987
Published
1987-10-00
Pages
3446-51
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367995
Subset
IM
Grants
NIGMS NIH HHS · GM-36659 · United States
External Links
PubMed source DOI: 10.1128/mcb.7.10.3446-3451.1987 PMC Full Text View journal details
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