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PMID: 1995588 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

A family of regulatory genes associated with type II restriction-modification systems.

Journal of bacteriology ·Vol. 173 ·No. 4 ·1991-02-00 ·Pages 1367-75

Tao T, Bourne JC, Blumenthal RM

Abstract

Restriction-modification systems must be regulated to avoid autorestriction and death of the host cell. An open reading frame (ORF) in the PvuII restriction-modification system appears to code for a regulatory protein from a previously unrecognized family. First, interruptions of this ORF result in a nonrestricting phenotype. Second, this ORF can restore restriction competence to such interrupted mutants in trans. Third, the predicted amino acid sequence of this ORF resembles those of known DNA-binding proteins and includes a probable helix-turn-helix motif. A survey of unattributed ORFs in 15 other type II restriction-modification systems revealed three that closely resemble the PvuII ORF. All four members of this putative regulatory gene family have a common position relative to the endonuclease genes, suggesting a common regulatory mechanism.

Related Genes
PvuII
MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA Restriction-Modification Enzymes/genetics Deoxyribonucleases, Type II Site-Specific/genetics Escherichia coli/genetics Gene Expression Regulation Genes, Regulator Molecular Sequence Data Open Reading Frames/genetics Plasmids Restriction Mapping Sequence Homology, Nucleic Acid
Chemicals
DNA Restriction-Modification Enzymes CAGCTG-specific type II deoxyribonucleases Deoxyribonucleases, Type II Site-Specific
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tao T
Department of Microbiology, Medical College of Ohio, Toledo 43699-0008.
Bourne J C
Blumenthal R M
References (37)
37 references, click to expand
  1. Characterization of a cloned Bacillus subtilis gene that inhibits sporulation in multiple copies.
    J Bacteriol. 1986 Nov;168(2):860-9 PMID: 3096962
  2. A possible role for DNA restriction in bacterial evolution.
    Microbiol Sci. 1986 Oct;3(10):296-9 PMID: 2856420
  3. Characterization of clones of the BamHI methyltransferase gene.
    Gene. 1988 Dec 25;74(1):35-6 PMID: 3248731
  4. Sequence, internal homology and high-level expression of the gene for a DNA-(cytosine N4)-methyltransferase, M.Pvu II.
    Nucleic Acids Res. 1989 Jun 12;17(11):4161-75 PMID: 2662138
  5. Structural basis of DNA-protein recognition.
    Trends Biochem Sci. 1989 Jul;14(7):286-90 PMID: 2672451
  6. Cloning, characterization and heterologous expression of the SmaI restriction-modification system.
    Nucleic Acids Res. 1989 Dec 11;17(23):9783-96 PMID: 2690008
  7. Purification, cloning and sequence analysis of RsrI DNA methyltransferase: lack of homology between two enzymes, RsrI and EcoRI, that methylate the same nucleotide in identical recognition sequences.
    Nucleic Acids Res. 1989 Dec 25;17(24):10403-25 PMID: 2690017
  8. Escherichia coli K-12 restricts DNA containing 5-methylcytosine.
    Proc Natl Acad Sci U S A. 1986 Dec;83(23):9070-4 PMID: 3024165
  9. The guanine and cytosine content of genomic DNA and bacterial evolution.
    Proc Natl Acad Sci U S A. 1987 Jan;84(1):166-9 PMID: 3467347
  10. Interaction of AluI, Cfr6I and PvuII restriction-modification enzymes with substrates containing either N4-methylcytosine or 5-methylcytosine.
    Biochim Biophys Acta. 1987 Aug 25;909(3):201-7 PMID: 3040102
  11. A rapid and convenient method for the preparation and storage of competent bacterial cells.
    Nucleic Acids Res. 1988 Apr 25;16(8):3580 PMID: 3287331
  12. A large family of bacterial activator proteins.
    Proc Natl Acad Sci U S A. 1988 Sep;85(18):6602-6 PMID: 3413113
  13. The helix-turn-helix DNA binding motif.
    J Biol Chem. 1989 Feb 5;264(4):1903-6 PMID: 2644244
  14. Nomenclature for bacterial genes coding for class-II restriction endonucleases and modification methyltransferases.
    Gene. 1988 Dec 25;74(1):279-80 PMID: 3266859
  15. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
    J Bacteriol. 1978 Jun;134(3):1141-56 PMID: 149110
  16. Two new restriction endonucleases from Proteus vulgaris.
    Nucleic Acids Res. 1981 Sep 25;9(18):4525-36 PMID: 6272209
  17. The N-terminal arms of lambda repressor wrap around the operator DNA.
    Nature. 1982 Jul 29;298(5873):441-3 PMID: 7088189
  18. The operator-binding domain of lambda repressor: structure and DNA recognition.
    Nature. 1982 Jul 29;298(5873):443-7 PMID: 7088190
  19. The isolation and characterization of the Escherichia coli DNA adenine methylase (dam) gene.
    Nucleic Acids Res. 1983 Feb 11;11(3):837-51 PMID: 6300769
  20. Structure of the operator-binding domain of bacteriophage lambda repressor: implications for DNA recognition and gene regulation.
    Cold Spring Harb Symp Quant Biol. 1983;47 Pt 1:435-40 PMID: 6305562
  21. Bacteriophage survival: multiple mechanisms for avoiding the deoxyribonucleic acid restriction systems of their hosts.
    Microbiol Rev. 1983 Sep;47(3):345-60 PMID: 6314109
  22. Characterization of the genes coding for the Eco RV restriction and modification system of Escherichia coli.
    Nucleic Acids Res. 1984 Apr 25;12(8):3659-76 PMID: 6328432
  23. The organization and complete nucleotide sequence of the PstI restriction-modification system.
    J Biol Chem. 1984 Jun 25;259(12):8015-26 PMID: 6330092
  24. DNA adenine methylation of GATC sequences appeared recently in the Escherichia coli lineage.
    J Bacteriol. 1984 Nov;160(2):586-90 PMID: 6094478
  25. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  26. Positive control of a regulon for defenses against oxidative stress and some heat-shock proteins in Salmonella typhimurium.
    Cell. 1985 Jul;41(3):753-62 PMID: 2988786
  27. A phage repressor-operator complex at 7 A resolution.
    Nature. 1985 Aug 15-21;316(6029):596-601 PMID: 4033757
  28. Changing the binding specificity of a repressor by redesigning an alpha-helix.
    Nature. 1985 Aug 15-21;316(6029):601-5 PMID: 4033758
  29. Nucleotide sequence and mutational analysis of an immunity repressor gene from Bacillus subtilis temperate phage phi 105.
    Nucleic Acids Res. 1985 Aug 12;13(15):5441-55 PMID: 2993999
  30. Cloning of a restriction-modification system from Proteus vulgaris and its use in analyzing a methylase-sensitive phenotype in Escherichia coli.
    J Bacteriol. 1985 Nov;164(2):501-9 PMID: 2997113
  31. Nucleotide sequence of the immunity region of Bacillus subtilis bacteriophage phi 105: identification of the repressor gene and its mRNA and protein products.
    Gene. 1985;38(1-3):153-64 PMID: 3934047
  32. A gene essential for Agrobacterium virulence is homologous to a family of positive regulatory loci.
    Proc Natl Acad Sci U S A. 1986 Nov;83(21):8278-82 PMID: 3022288
  33. TraY proteins of F and related episomes are members of the Arc and Mnt repressor family.
    J Mol Biol. 1990 Jan 5;211(1):5-6 PMID: 2299672
  34. Comparison of the nucleotide and amino acid sequences of the RsrI and EcoRI restriction endonucleases.
    Gene. 1989 Dec 21;85(1):1-13 PMID: 2695392
  35. Rapid and sensitive sequence comparison with FASTP and FASTA.
    Methods Enzymol. 1990;183:63-98 PMID: 2156132
  36. A family of positive regulators related to the Pseudomonas putida TOL plasmid XylS and the Escherichia coli AraC activators.
    Nucleic Acids Res. 1990 Apr 25;18(8):2149-52 PMID: 2186376
  37. Cloned restriction-modification systems--a review.
    Gene. 1988 Dec 25;74(1):281-9 PMID: 3074014
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.4.1367-1375.1991
Published
1991-02-00
Pages
1367-75
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC207272
Subset
IM
Databases
GENBANK
M63619, M63620, M63621, M63622, M77223, S70358, S70359, S70363, S70365, S70370
External Links
PubMed source DOI: 10.1128/jb.173.4.1367-1375.1991 PMC Full Text View journal details
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