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PMID: 20038682 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

The Chlamydomonas reinhardtii BBSome is an IFT cargo required for export of specific signaling proteins from flagella.

The Journal of cell biology ·Vol. 187 ·No. 7 ·2009-12-28 ·Pages 1117-32

Lechtreck KF, Johnson EC, Sakai T, Cochran D, Ballif BA, Rush J, Pazour GJ, Ikebe M, Witman GB

Abstract

In humans, seven evolutionarily conserved genes that cause the cilia-related disorder Bardet-Biedl syndrome (BBS) encode proteins that form a complex termed the BBSome. The function of the BBSome in the cilium is not well understood. We purified a BBSome-like complex from Chlamydomonas reinhardtii flagella and found that it contains at least BBS1, -4, -5, -7, and -8 and undergoes intraflagellar transport (IFT) in association with a subset of IFT particles. C. reinhardtii insertional mutants defective in BBS1, -4, and -7 assemble motile, full-length flagella but lack the ability to phototax. In the bbs4 mutant, the assembly and transport of IFT particles are unaffected, but the flagella abnormally accumulate several signaling proteins that may disrupt phototaxis. We conclude that the BBSome is carried by IFT but is an adapter rather than an integral component of the IFT machinery. C. reinhardtii BBS4 may be required for the export of signaling proteins from the flagellum via IFT.

MeSH Terms
Algal Proteins/genetics,metabolism,physiology Axoneme/genetics,physiology Chlamydomonas reinhardtii/genetics,metabolism Flagella/metabolism Protein Transport/physiology Signal Transduction
Chemicals
Algal Proteins
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Lechtreck Karl-Ferdinand
Department of Cell Biology, University of Massachusetts Medical School, Worcester, 01655, USA. [email protected]
Johnson Eric C
Sakai Tsuyoshi
Cochran Deborah
Ballif Bryan A
Rush John
Pazour Gregory J
Ikebe Mitsuo
Witman George B
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
1540-8140
DOI
10.1083/jcb.200909183
Email
[email protected]
Published
2009-12-28
Pages
1117-32
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2806276
Subset
IM
Grants
NIGMS NIH HHS · GM060992 · United States
NIGMS NIH HHS · R01 GM030626 · United States
NIGMS NIH HHS · R37 GM030626 · United States
NIGMS NIH HHS · GM030626 · United States
NIAMS NIH HHS · AR048526 · United States
NIDDK NIH HHS · DK32520 · United States
NIAMS NIH HHS · R01 AR048526 · United States
NIGMS NIH HHS · R01 GM060992 · United States
NCRR NIH HHS · P20 RR016462 · United States
NIAMS NIH HHS · AR048898 · United States
NCRR NIH HHS · P20 RR16462 · United States
NIDCD NIH HHS · R01 DC006103 · United States
NIDDK NIH HHS · P30 DK032520 · United States
NIDCD NIH HHS · DC006103 · United States
NIAMS NIH HHS · R01 AR048898 · United States
External Links
PubMed source DOI: 10.1083/jcb.200909183 PMC Full Text View journal details
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