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PMID: 2017145 Published · ppublish English Comparative Study Journal Article

Cloning and sequencing of an Escherichia coli K12 gene which encodes a polypeptide having similarity to the human ferritin H subunit.

Molecular & general genetics : MGG ·Vol. 225 ·No. 3 ·1991-03-00 ·Pages 510-3

Izuhara M, Takamune K, Takata R

Abstract

Using lambda phage clones containing segments of the Escherichia coli K12 chromosome as hybridization probes, we found one gene at 42 min on the E. coli chromosome map, the expression of which was affected by RNase III. The sequence of the DNA fragment containing this gene (gen-165) revealed the presence of an open reading frame encoding a polypeptide of 165 amino acid residues. The amino acid sequence deduced from the nucleotide sequence exhibited a remarkable similarity to that of the human ferritin H chain.

Related Genes
gen-165
MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Northern Chromosome Mapping Chromosomes, Bacterial Cloning, Molecular DNA DNA, Bacterial Endoribonucleases/metabolism Escherichia coli/genetics Escherichia coli Proteins Ferritins/genetics Genes, Bacterial Humans Molecular Sequence Data Open Reading Frames Restriction Mapping Ribonuclease III Sequence Homology, Nucleic Acid
Chemicals
DNA, Bacterial Escherichia coli Proteins DNA Ferritins Endoribonucleases Ribonuclease III ribonuclease III, E coli
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Izuhara M
Department of Biology, Saga Medical School, Nabeshima, Japan.
Takamune K
Takata R
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19 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00261694
Published
1991-03-00
Pages
510-3
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
M64566, S63796, S63798, S79268, S79270, S79277, S85222, S85224, X53513, X59005
External Links
PubMed source DOI: 10.1007/BF00261694
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