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PMID: 2027746 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

rar mutations which increase artificial chromosome stability in Saccharomyces cerevisiae identify transcription and recombination proteins.

Nucleic acids research ·Vol. 19 ·No. 7 ·1991-04-11 ·Pages 1385-91

Kipling D, Tambini C, Kearsey SE

Abstract

In an attempt to identify trans-acting factors involved in replication origin function, we have characterized the RAR3 and RAR5 genes, identified by mutations which increase the mitotic stability of artificial chromosomes whose replication is dependent on the activity of weak ARS elements. Sequence analysis has shown that the RAR3 gene is identical to GAL11/SPT13, which encodes a putative transcription factor involved in the expression of a wide range of genes. Change-of-function mutations that truncate the RAR3 protein appear to be required to enhance chromosome stability. In contrast, loss of the RAR5 protein results in enhanced chromosome stability, as if the protein is an inhibitor of ARS function. The RAR5 gene encodes the 175 kDa DNA strand transfer protein beta, an activity that can promote the transfer of a strand from a double-stranded DNA molecule to a complementary single strand. This observation implies that a presumed recombination activity can affect eukaryotic chromosomal replication.

Related Genes
RAR3 RAR5
MeSH Terms
Chromosomes, Fungal Cloning, Molecular Fungal Proteins/genetics Galactose/genetics Gene Library Genes, Fungal Genome, Human Humans Molecular Sequence Data Mutation Phenotype Plasmids Recombination, Genetic Restriction Mapping Saccharomyces cerevisiae/genetics Transcription Factors/genetics Transcription, Genetic
Chemicals
Fungal Proteins Transcription Factors Galactose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kipling D
Department of Zoology, University of Oxford, UK.
Tambini C
Kearsey S E
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.7.1385
Published
1991-04-11
Pages
1385-91
Language
English
Region
England
NLM ID
0411011
PMCID
PMC333890
Subset
IM
Databases
GENBANK
M36725
External Links
PubMed source DOI: 10.1093/nar/19.7.1385 PMC Full Text View journal details
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