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PMID: 2928110 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Anti-OTF-1 antibodies inhibit NFIII stimulation of in vitro adenovirus DNA replication.

Nucleic acids research ·Vol. 17 ·No. 5 ·1989-03-11 ·Pages 1845-63

Pruijn JM, van der Vliet PC, Dathan NA, Mattaj IW

Abstract

HeLa cell OTF-1 has been purified on the basis of its DNA binding activity and used to raise a polyclonal rabbit antiserum. This antiserum is shown to recognize both native and denatured OTF-1 from both human and a similar protein from Xenopus culture cells, but to react either more weakly or not at all with the lymphoid cell-specific OTF-2. Separately, NFIII has been purified on the basis of its ability to stimulate Adenovirus DNA replication in vitro. On denaturing polyacrylamide gels OTF-1 and NFIII exhibit identical mobility. Anti-OTF-1 antiserum recognizes NFIII and neutralizes its stimulatory effect on DNA replication. Moreover, OTF-1 can functionally replace NFIII. Taken together with previously published DNA binding data, this indicates that OTF-1 and NFIII are either very closely related or identical.

MeSH Terms
Adenoviruses, Human/genetics Animals Antibodies/physiology Antigen-Antibody Reactions Binding, Competitive DNA, Viral/biosynthesis HeLa Cells Humans Immune Sera/pharmacology Nuclear Proteins/immunology,isolation & purification,physiology Transcription Factors/immunology,isolation & purification Xenopus
Chemicals
Antibodies DNA, Viral Immune Sera Nuclear Proteins Transcription Factors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Pruijn J M
Laboratory for Physiological Chemistry, State University of Utrecht, The Netherlands.
van der Vliet P C
Dathan N A
Mattaj I W
References (62)
62 references, click to expand
  1. Binding of a simian virus 40 T antigen-related protein to DNA.
    J Mol Biol. 1981 Jan 25;145(3):471-88 PMID: 6267291
  2. The ubiquitous octamer-binding protein Oct-1 contains a POU domain with a homeo box subdomain.
    Genes Dev. 1988 Dec;2(12A):1582-99 PMID: 2905684
  3. Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.
    Nucleic Acids Res. 1981 Dec 11;9(23):6505-25 PMID: 6275366
  4. Independently evolving chicken histone H2B genes: identification of a ubiquitous H2B-specific 5' element.
    Nucleic Acids Res. 1982 Dec 11;10(23):7851-63 PMID: 6296794
  5. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
    Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 PMID: 6828386
  6. Production of antibodies by inoculation into lymph nodes.
    Methods Enzymol. 1983;93:3-12 PMID: 6346012
  7. Structure of the 5' ends of immunoglobulin genes: a novel conserved sequence.
    Proc Natl Acad Sci U S A. 1984 May;81(9):2650-4 PMID: 6425835
  8. Correct transcription of an immunoglobulin kappa gene requires an upstream fragment containing conserved sequence elements.
    Nature. 1984 Jul 5-11;310(5972):71-4 PMID: 6330567
  9. Nuclear segregation of U2 snRNA requires binding of specific snRNP proteins.
    Cell. 1985 Jan;40(1):111-8 PMID: 2578319
  10. Stimulation of in vitro transcription from the SV40 early promoter by the enhancer involves a specific trans-acting factor.
    EMBO J. 1984 Dec 20;3(13):3129-33 PMID: 6098465
  11. A viral enhancer element specifically active in human haematopoietic cells.
    Nature. 1985 Jun 13-19;315(6020):597-600 PMID: 2989696
  12. An enhancer-like sequence within the Xenopus U2 gene promoter facilitates the formation of stable transcription complexes.
    Nature. 1985 Jul 11-17;316(6024):163-7 PMID: 2409453
  13. Orientation-dependent transcriptional activator upstream of a human U2 snRNA gene.
    Mol Cell Biol. 1985 Jul;5(7):1560-70 PMID: 2410771
  14. The two embryonic U1 RNA genes of Xenopus laevis have both common and gene-specific transcription signals.
    EMBO J. 1985 Jun;4(6):1529-35 PMID: 2411540
  15. Transcription signals in embryonic Xenopus laevis U1 RNA genes.
    EMBO J. 1985 Jun;4(6):1537-43 PMID: 2411541
  16. A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes.
    Nature. 1986 Jan 9-15;319(6049):154-8 PMID: 3079885
  17. Inhibition of adenovirus DNA replication in vitro by autoimmune sera.
    Eur J Biochem. 1986 Jan 15;154(2):363-70 PMID: 3943534
  18. Purification of nuclear factor I by DNA recognition site affinity chromatography.
    J Biol Chem. 1986 Jan 25;261(3):1398-408 PMID: 3003068
  19. Affinity purification of sequence-specific DNA binding proteins.
    Proc Natl Acad Sci U S A. 1986 Aug;83(16):5889-93 PMID: 3461465
  20. Nuclear factor III, a novel sequence-specific DNA-binding protein from HeLa cells stimulating adenovirus DNA replication.
    Nature. 1986 Aug 14-20;322(6080):656-9 PMID: 3748145
  21. Interaction of a common factor with conserved promoter and enhancer sequences in histone H2B, immunoglobulin, and U2 small nuclear RNA (snRNA) genes.
    Proc Natl Acad Sci U S A. 1986 Sep;83(17):6382-6 PMID: 3462701
  22. A cellular DNA-binding protein that activates eukaryotic transcription and DNA replication.
    Cell. 1987 Jan 16;48(1):79-89 PMID: 3024847
  23. Multiple sequence elements are required for maximal in vitro transcription of a human histone H2B gene.
    Mol Cell Biol. 1986 Oct;6(10):3329-40 PMID: 3025588
  24. A transcription factor which binds to the enhancers of SV40, immunoglobulin heavy chain and U2 snRNA genes.
    Nature. 1987 Jan 15-21;325(6101):268-72 PMID: 3027566
  25. Nuclear factors binding specific sequences within the immunoglobulin enhancer interact differentially with other enhancer elements.
    EMBO J. 1986 Dec 1;5(12):3251-8 PMID: 3102227
  26. Sequence-specific interactions between cellular DNA-binding proteins and the adenovirus origin of DNA replication.
    Mol Cell Biol. 1987 Feb;7(2):875-86 PMID: 3821731
  27. The immunoglobulin octanucleotide: independent activity and selective interaction with enhancers.
    Science. 1987 Mar 20;235(4795):1498-501 PMID: 3029871
  28. Discrete elements within the SV40 enhancer region display different cell-specific enhancer activities.
    EMBO J. 1987 Apr;6(4):1017-25 PMID: 3036487
  29. An octamer oligonucleotide upstream of a TATA motif is sufficient for lymphoid-specific promoter activity.
    Nature. 1987 Sep 10-16;329(6135):174-8 PMID: 3627254
  30. Only two of the four sites of interaction with nuclear factors within the Xenopus U2 gene promoter are necessary for efficient transcription.
    Nucleic Acids Res. 1987 Aug 25;15(16):6437-53 PMID: 3627994
  31. A common octamer motif binding protein is involved in the transcription of U6 snRNA by RNA polymerase III and U2 snRNA by RNA polymerase II.
    Cell. 1987 Oct 9;51(1):71-9 PMID: 3652209
  32. Purification and characterization of OTF-1, a transcription factor regulating cell cycle expression of a human histone H2b gene.
    Cell. 1987 Dec 4;51(5):773-81 PMID: 3677172
  33. Identification and purification of a human lymphoid-specific octamer-binding protein (OTF-2) that activates transcription of an immunoglobulin promoter in vitro.
    Cell. 1987 Dec 4;51(5):783-93 PMID: 3119226
  34. In vitro binding of cell-specific and ubiquitous nuclear proteins to the octamer motif of the SV40 enhancer and related motifs present in other promoters and enhancers.
    EMBO J. 1987 Oct;6(10):3015-25 PMID: 2826127
  35. DNA replication.
    J Biol Chem. 1988 Jan 5;263(1):1-4 PMID: 3275635
  36. Purification and characterization of nuclear factor III (origin recognition protein C), a sequence-specific DNA binding protein required for efficient initiation of adenovirus DNA replication.
    J Biol Chem. 1988 Jan 15;263(2):931-7 PMID: 2826468
  37. Promoter and enhancer elements containing a conserved sequence motif are recognized by nuclear factor III, a protein stimulating adenovirus DNA replication.
    EMBO J. 1987 Dec 1;6(12):3771-8 PMID: 3428274
  38. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  39. Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes.
    J Natl Cancer Inst. 1972 Sep;49(3):891-5 PMID: 4567231
  40. DNAse footprinting: a simple method for the detection of protein-DNA binding specificity.
    Nucleic Acids Res. 1978 Sep;5(9):3157-70 PMID: 212715
  41. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  42. Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes.
    Anal Biochem. 1980 Nov 15;109(1):76-86 PMID: 6258458
  43. The mouse immunoglobulin heavy-chain enhancer: effect on transcription in vitro and binding of proteins present in HeLa and lymphoid B cell extracts.
    EMBO J. 1986 Aug;5(8):1791-7 PMID: 3019664
  44. Cell-type specific protein binding to the enhancer of simian virus 40 in nuclear extracts.
    Nature. 1986 Oct 9-15;323(6088):544-8 PMID: 3020434
  45. Interaction of cell-type-specific nuclear proteins with immunoglobulin VH promoter region sequences.
    Nature. 1986 Oct 9-15;323(6088):548-51 PMID: 3093895
  46. A lymphoid-specific protein binding to the octamer motif of immunoglobulin genes.
    Nature. 1986 Oct 16-22;323(6089):640-3 PMID: 3095662
  47. Upstream regulatory sequences of immunoglobulin genes are recognized by nuclear proteins which also bind to other gene regions.
    Nucleic Acids Res. 1986 Nov 25;14(22):8829-44 PMID: 3786139
  48. The SV40 enhancer can be dissected into multiple segments, each with a different cell type specificity.
    Genes Dev. 1987 Mar;1(1):65-74 PMID: 2828161
  49. A 100-kD HeLa cell octamer binding protein (OBP100) interacts differently with two separate octamer-related sequences within the SV40 enhancer.
    Genes Dev. 1987 Dec;1(10):1147-60 PMID: 2828167
  50. Murine genomic DNA sequences replicating autonomously in mouse L cells.
    Cell. 1988 Feb 12;52(3):355-65 PMID: 2830982
  51. Transcriptional elements as components of eukaryotic origins of DNA replication.
    Cell. 1988 Mar 11;52(5):635-8 PMID: 3278812
  52. Human CCAAT-binding proteins have heterologous subunits.
    Cell. 1988 Apr 8;53(1):11-24 PMID: 3349524
  53. High expression of functional adenovirus DNA polymerase and precursor terminal protein using recombinant vaccinia virus.
    Nucleic Acids Res. 1988 Mar 25;16(6):2431-44 PMID: 3362670
  54. An ACCC-containing protein-binding sequence in the neighbourhood of the decanucleotide recognition site of the immunoglobulin gene promoter.
    Nucleic Acids Res. 1988 May 11;16(9):3693-704 PMID: 3131743
  55. A quantitative analysis of nuclear factor I/DNA interactions.
    Nucleic Acids Res. 1988 May 25;16(10):4419-35 PMID: 3380685
  56. A family of human CCAAT-box-binding proteins active in transcription and DNA replication: cloning and expression of multiple cDNAs.
    Nature. 1988 Jul 21;334(6179):218-24 PMID: 3398920
  57. Interaction between the octamer-binding protein nuclear factor III and the adenovirus origin of DNA replication.
    J Virol. 1988 Sep;62(9):3092-102 PMID: 2841465
  58. Transcription factor OTF-1 is functionally identical to the DNA replication factor NF-III.
    Science. 1988 Sep 2;241(4870):1210-3 PMID: 3413485
  59. A human protein specific for the immunoglobulin octamer DNA motif contains a functional homeobox domain.
    Cell. 1988 Oct 7;55(1):135-44 PMID: 2901913
  60. A cloned octamer transcription factor stimulates transcription from lymphoid-specific promoters in non-B cells.
    Nature. 1988 Dec 8;336(6199):544-51 PMID: 2904653
  61. A human lymphoid-specific transcription factor that activates immunoglobulin genes is a homoeobox protein.
    Nature. 1988 Dec 8;336(6199):551-7 PMID: 2904654
  62. A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory system.
    Nucleic Acids Res. 1981 Jul 10;9(13):3047-60 PMID: 6269071
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.5.1845
Published
1989-03-11
Pages
1845-63
Language
English
Region
England
NLM ID
0411011
PMCID
PMC317527
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/17.5.1845 PMC Full Text View journal details
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