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PMID: 3131743 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

An ACCC-containing protein-binding sequence in the neighbourhood of the decanucleotide recognition site of the immunoglobulin gene promoter.

Nucleic acids research ·Vol. 16 ·No. 9 ·1988-05-11 ·Pages 3693-704

Mocikat R, Pruijn GJ, van der Vliet PC, Zachau HG

Abstract

The decanucleotide (dc) box TNATTTGCAT is an important functional element in the K light chain gene promoter and was shown to bind nuclear proteins. We now describe the binding of nuclear factor III to dc containing oligonucleotides and define a second protein binding site in the neighbourhood of dc which turned out to be recognized by a partially purified NFIII preparation but not by highly purified NFIII (abbreviations ref. 1). This site comprises an ACCC-containing sequence element. It was further characterized by the hydroxyl radical footprinting method which is applicable to 40 bp long oligonucleotides. The significance of the ACCC-containing sequence and of the previously described 15mer sequence pd is discussed.

MeSH Terms
Adenine Animals Base Sequence Cell Line Cytosine Genes, Immunoglobulin Immunoglobulin Light Chains/genetics Immunoglobulin kappa-Chains/genetics Molecular Sequence Data Nuclear Proteins/metabolism Oligodeoxyribonucleotides/metabolism Promoter Regions, Genetic Protein Binding
Chemicals
Immunoglobulin Light Chains Immunoglobulin kappa-Chains Nuclear Proteins Oligodeoxyribonucleotides Cytosine Adenine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Mocikat R
Institut für Physiologische Chemie, Physikalische Biochemie und Zellbiologie, Universität München, FRG.
Pruijn G J
van der Vliet P C
Zachau H G
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.9.3693
Published
1988-05-11
Pages
3693-704
Language
English
Region
England
NLM ID
0411011
PMCID
PMC336550
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/16.9.3693 PMC Full Text View journal details
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