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PMID: 3627994 Published · ppublish English Comparative Study Journal Article

Only two of the four sites of interaction with nuclear factors within the Xenopus U2 gene promoter are necessary for efficient transcription.

Nucleic acids research ·Vol. 15 ·No. 16 ·1987-08-25 ·Pages 6437-53

Tebb G, Bohmann D, Mattaj IW

Abstract

An analysis, performed by DNase I footprinting, of the interactions between factors present in Molt-4 nuclear extracts and a Xenopus U2 snRNA gene promoter is presented. Four distinct regions of sequence-specific DNA-factor interaction are found. Two of these correspond to the previously identified proximal and distal sequence elements (PSE and DSE) of the promoter. Both of these elements are important in U2 transcription, indicating a functional role for the observed interactions. The other two sites of interaction correspond to a sequence element conserved in many, but not all, vertebrate U snRNA gene promoters (the MSE) and to a region adjacent to the site of transcription initiation (the "cap site"). Site-directed mutants of these latter two elements are constructed which no longer bind nuclear factors. Transcriptional analysis in Xenopus oocytes reveals that these mutants are transcribed as efficiently as wild-type U2. Other possible roles for the two factors are discussed.

MeSH Terms
Animals Base Sequence DNA/genetics,metabolism Promoter Regions, Genetic RNA Caps/genetics RNA, Small Nuclear/genetics Transcription Factors/metabolism Transcription, Genetic Vertebrates/genetics Xenopus laevis/genetics
Chemicals
RNA Caps RNA, Small Nuclear Transcription Factors DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tebb G
Bohmann D
Mattaj I W
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53 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.16.6437
Published
1987-08-25
Pages
6437-53
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306115
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/15.16.6437 PMC Full Text View journal details
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