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PMID: 3031597 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Properties of a U1 RNA enhancer-like sequence.

Nucleic acids research ·Vol. 15 ·No. 6 ·1987-03-25 ·Pages 2403-16

Ciliberto G, Palla F, Tebb G, Mattaj IW, Philipson L

Abstract

The properties of a X.laevis U1B snRNA gene enhancer have been studied by microinjection in Xenopus oocytes. The enhancer-like sequence, defined as a short DNA stretch that is able to activate transcription in an orientation independent manner, is interchangeable between different U snRNA genes. The enhancer sequence alone does not, however, efficiently activate transcription from an SV40 pol II promoter but regains its activity when combined with the U-gene specific proximal sequence element. DNase I protection experiments show that the X.laevis U1B enhancer can interact specifically with a nuclear factor present in mammalian cells.

MeSH Terms
Animals Base Sequence DNA Restriction Enzymes Enhancer Elements, Genetic Female Genes Genes, Regulator Oocytes/metabolism Plasmids Promoter Regions, Genetic RNA, Small Nuclear/genetics Simian virus 40/genetics Transcription, Genetic Xenopus laevis
Chemicals
RNA, Small Nuclear DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Ciliberto G
Palla F
Tebb G
Mattaj I W
Philipson L
References (36)
36 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.6.2403
Published
1987-03-25
Pages
2403-16
Language
English
Region
England
NLM ID
0411011
PMCID
PMC340659
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/15.6.2403 PMC Full Text View journal details
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