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PMID: 2347308 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The DNA binding domain (POU domain) of transcription factor oct-1 suffices for stimulation of DNA replication.

The EMBO journal ·Vol. 9 ·No. 6 ·1990-06-00 ·Pages 1883-8

Verrijzer CP, Kal AJ, Van der Vliet PC

Abstract

Oct-1, also referred to as NFIII, OTF-1, OBP100 or NF-A1, is a ubiquitous sequence-specific DNA binding protein that activates transcription and adenovirus DNA replication. The protein contains a conserved DNA binding domain (POU domain) present in several transcription factors. We have overproduced oct-1, the related oct-2 and several oct-1 deletion mutants in a vaccinia expression system to identify the domains important for activation of DNA replication in vitro. Both oct-1 and oct-2 stimulate adenovirus DNA replication in an octamer-dependent manner. From deletion studies it appears that the 160 amino acid long POU domain suffices for stimulation. This domain consists of two subdomains, a POU-specific and a homeo domain. Deletion of the POU-specific domain revealed that the homeo domain has an intrinsic, but weak DNA binding activity and surprisingly, inhibits DNA replication. As the POU domain does not coincide with the transcription activation domain, these results indicate that, although oct-1 functions both in DNA replication and transcription, the mechanisms underlying these processes are probably distinct.

MeSH Terms
Adenoviruses, Human/genetics,growth & development Base Sequence DNA Replication DNA, Recombinant/metabolism DNA, Viral/metabolism DNA-Binding Proteins/genetics,metabolism Deoxyribonuclease I HeLa Cells Host Cell Factor C1 Humans Molecular Sequence Data Mutation Nucleic Acid Conformation Octamer Transcription Factor-1 Transcription Factors/genetics,metabolism Transfection Virus Replication
Chemicals
DNA, Recombinant DNA, Viral DNA-Binding Proteins HCFC1 protein, human Host Cell Factor C1 Octamer Transcription Factor-1 POU2F1 protein, human Transcription Factors Deoxyribonuclease I
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Verrijzer C P
Laboratory for Physiological Chemistry, University of Utrecht, The Netherlands.
Kal A J
Van der Vliet P C
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43 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-06-00
Pages
1883-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC551894
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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