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PMID: 2040648 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification of a carbohydrate-based endothelial ligand for a lymphocyte homing receptor.

The Journal of cell biology ·Vol. 113 ·No. 5 ·1991-06-00 ·Pages 1213-21

Imai Y, Singer MS, Fennie C, Lasky LA, Rosen SD

Abstract

Lymphocyte attachment to high endothelial venules within lymph nodes is mediated by the peripheral lymph node homing receptor (pnHR), originally defined on mouse lymphocytes by the MEL-14 mAb. The pnHR is a calcium-dependent lectin-like receptor, a member of the LEC-CAM family of adhesion proteins. Here, using a soluble recombinant form of the homing receptor, we have identified an endothelial ligand for the pnHR as an approximately 50-kD sulfated, fucosylated, and sialylated glycoprotein, which we designate Sgp50 (sulfated glycoprotein of 50 kD). Recombinant receptor binding to this lymph node-specific glycoprotein requires calcium and is inhibitable by specific carbohydrates and by MEL-14 mAb. Sialylation of the component is required for binding. Additionally, the glycoprotein is precipitated by MECA-79, an adhesion-blocking mAb reactive with lymph node HEV. A related glycoprotein of approximately 90 kD (designated as Sgp90) is also identified.

MeSH Terms
Animals Carbohydrate Metabolism Cell Adhesion Cell Adhesion Molecules/biosynthesis,isolation & purification,physiology Endothelium, Vascular/physiology Female Ligands Lymph Nodes/physiology Lymphocytes/immunology,physiology Mice Mice, Inbred ICR Molecular Weight Receptors, Lymphocyte Homing/physiology Sulfates/metabolism Sulfur Radioisotopes Tritium
Chemicals
Cell Adhesion Molecules Ligands Receptors, Lymphocyte Homing Sulfates Sulfur Radioisotopes Tritium
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Imai Y
Department of Anatomy, University of California, San Francisco 94143-0452.
Singer M S
Fennie C
Lasky L A
Rosen S D
References (52)
52 references, click to expand
  1. Organ specificity of lymphocyte migration: mediation by highly selective lymphocyte interaction with organ-specific determinants on high endothelial venules.
    Eur J Immunol. 1980 Jul;10(7):556-61 PMID: 6157544
  2. Leu-8/TQ1 is the human equivalent of the Mel-14 lymph node homing receptor.
    Nature. 1989 Nov 2;342(6245):78-82 PMID: 2509939
  3. Possible role for cell-surface carbohydrate-binding molecules in lymphocyte recirculation.
    J Cell Biol. 1983 Mar;96(3):722-9 PMID: 6833380
  4. A cell-surface molecule involved in organ-specific homing of lymphocytes.
    Nature. 1983 Jul 7-13;304(5921):30-4 PMID: 6866086
  5. Phosphomannosyl receptors may participate in the adhesive interaction between lymphocytes and high endothelial venules.
    J Cell Biol. 1984 Oct;99(4 Pt 1):1535-40 PMID: 6090473
  6. Involvement of sialic acid on endothelial cells in organ-specific lymphocyte recirculation.
    Science. 1985 May 24;228(4702):1005-7 PMID: 4001928
  7. Lymphocyte homing receptors.
    Cell. 1986 Mar 14;44(5):673-80 PMID: 3004743
  8. The regulation of lymphocyte traffic.
    Curr Top Microbiol Immunol. 1986;128:85-122 PMID: 3533449
  9. Phosphomannosyl-derivatized beads detect a receptor involved in lymphocyte homing.
    J Cell Biol. 1987 Mar;104(3):713-23 PMID: 2950121
  10. Receptors involved in lymphocyte homing: relationship between a carbohydrate-binding receptor and the MEL-14 antigen.
    J Cell Biol. 1987 Mar;104(3):725-31 PMID: 2950122
  11. Specific cell-adhesion mechanisms determining migration pathways of recirculating lymphocytes.
    Annu Rev Immunol. 1987;5:201-22 PMID: 3297104
  12. Isolation and culture of high endothelial cells from rat lymph nodes.
    J Cell Sci. 1987 Feb;87 ( Pt 1):133-44 PMID: 3312249
  13. Homing receptors on human and rodent lymphocytes--evidence for a conserved carbohydrate-binding specificity.
    Blood. 1987 Dec;70(6):1842-50 PMID: 2823939
  14. Asparagine-linked oligosaccharides on lutropin, follitropin, and thyrotropin. I. Structural elucidation of the sulfated and sialylated oligosaccharides on bovine, ovine, and human pituitary glycoprotein hormones.
    J Biol Chem. 1988 Jan 5;263(1):25-35 PMID: 3121609
  15. A tissue-specific endothelial cell molecule involved in lymphocyte homing.
    Nature. 1988 Jan 7;331(6151):41-6 PMID: 3340147
  16. Sulfated N-linked oligosaccharides in mammalian cells. I. Complex-type chains with sialic acids and O-sulfate esters.
    J Biol Chem. 1988 Jun 25;263(18):8879-89 PMID: 3379050
  17. Two distinct classes of carbohydrate-recognition domains in animal lectins.
    J Biol Chem. 1988 Jul 15;263(20):9557-60 PMID: 3290208
  18. Molecular mechanisms underlying lymphocyte recirculation. I. Functional, phenotypical and morphological characterization of high endothelial cells cultured in vitro.
    Eur J Immunol. 1988 Aug;18(8):1235-44 PMID: 3046950
  19. Immunohistologic and functional characterization of a vascular addressin involved in lymphocyte homing into peripheral lymph nodes.
    J Cell Biol. 1988 Nov;107(5):1853-62 PMID: 2460470
  20. A pituitary N-acetylgalactosamine transferase that specifically recognizes glycoprotein hormones.
    Science. 1988 Nov 11;242(4880):930-3 PMID: 2460923
  21. Designing CD4 immunoadhesins for AIDS therapy.
    Nature. 1989 Feb 9;337(6207):525-31 PMID: 2536900
  22. Intravenously injected sialidase inactivates attachment sites for lymphocytes on high endothelial venules.
    J Immunol. 1989 Mar 15;142(6):1895-902 PMID: 2921520
  23. Endothelial leukocyte adhesion molecule 1: an inducible receptor for neutrophils related to complement regulatory proteins and lectins.
    Science. 1989 Mar 3;243(4895):1160-5 PMID: 2466335
  24. Mouse lymph node homing receptor cDNA clone encodes a glycoprotein revealing tandem interaction domains.
    Science. 1989 Mar 3;243(4895):1165-72 PMID: 2646713
  25. Lymphocyte homing.
    Adv Immunol. 1989;44:313-78 PMID: 2646861
  26. Cloning of GMP-140, a granule membrane protein of platelets and endothelium: sequence similarity to proteins involved in cell adhesion and inflammation.
    Cell. 1989 Mar 24;56(6):1033-44 PMID: 2466574
  27. Cloning of a lymphocyte homing receptor reveals a lectin domain.
    Cell. 1989 Mar 24;56(6):1045-55 PMID: 2647302
  28. Adhesion molecules controlling lymphocyte migration.
    Cell. 1989 Mar 24;56(6):907-10 PMID: 2647304
  29. Characterization of a human homologue of the murine peripheral lymph node homing receptor.
    J Cell Biol. 1989 Jul;109(1):421-7 PMID: 2663882
  30. Requirement for sialic acid on the endothelial ligand of a lymphocyte homing receptor.
    J Cell Biol. 1990 Dec;111(6 Pt 1):2757-64 PMID: 2277086
  31. Carbohydrate ligands for endothelial-leukocyte adhesion molecule 1.
    Proc Natl Acad Sci U S A. 1991 Feb 15;88(4):1138-42 PMID: 1705026
  32. The Mel 14 antibody binds to the lectin domain of the murine peripheral lymph node homing receptor.
    J Cell Biol. 1990 Jan;110(1):147-53 PMID: 1688560
  33. Separation and analysis of glycoprotein oligosaccharides.
    Methods Cell Biol. 1989;32:141-83 PMID: 2691849
  34. Warner-Lambert/Parke-Davis Award lecture. Cellular and molecular mechanisms that direct leukocyte traffic.
    Am J Pathol. 1990 Jan;136(1):3-11 PMID: 2404417
  35. Rapid neutrophil adhesion to activated endothelium mediated by GMP-140.
    Nature. 1990 Feb 22;343(6260):757-60 PMID: 1689464
  36. Lymphocyte homing: progress and prospects.
    Curr Opin Cell Biol. 1989 Oct;1(5):913-9 PMID: 2697294
  37. Identification of a human peripheral lymph node homing receptor: a rapidly down-regulated adhesion molecule.
    Proc Natl Acad Sci U S A. 1990 Mar;87(6):2244-8 PMID: 2179952
  38. The mouse lymph node homing receptor is identical with the lymphocyte cell surface marker Ly-22: role of the EGF domain in endothelial binding.
    Cell. 1990 May 18;61(4):611-22 PMID: 1693096
  39. A homing receptor-IgG chimera as a probe for adhesive ligands of lymph node high endothelial venules.
    J Cell Biol. 1990 Jun;110(6):2221-9 PMID: 2190992
  40. Adhesion receptors of the immune system.
    Nature. 1990 Aug 2;346(6283):425-34 PMID: 1974032
  41. Direct demonstration of the lectin activity of gp90MEL, a lymphocyte homing receptor.
    J Cell Biol. 1990 Sep;111(3):1225-32 PMID: 2202735
  42. ELAM-1--dependent cell adhesion to vascular endothelium determined by a transfected human fucosyltransferase cDNA.
    Cell. 1990 Nov 2;63(3):475-84 PMID: 1699667
  43. Requirement for sialic acid on neutrophils in a GMP-140 (PADGEM) mediated adhesive interaction with activated platelets.
    Biochem Biophys Res Commun. 1990 Nov 15;172(3):1349-56 PMID: 1700907
  44. ELAM-1 mediates cell adhesion by recognition of a carbohydrate ligand, sialyl-Lex.
    Science. 1990 Nov 23;250(4984):1130-2 PMID: 1701274
  45. Recognition by ELAM-1 of the sialyl-Lex determinant on myeloid and tumor cells.
    Science. 1990 Nov 23;250(4984):1132-5 PMID: 1701275
  46. Isolation and chromosomal localization of cDNAs encoding a novel human lymphocyte cell surface molecule, LAM-1. Homology with the mouse lymphocyte homing receptor and other human adhesion proteins.
    J Exp Med. 1989 Jul 1;170(1):123-33 PMID: 2473156
  47. Human homologue of mouse lymph node homing receptor: evolutionary conservation at tandem cell interaction domains.
    Proc Natl Acad Sci U S A. 1989 Jul;86(14):5562-6 PMID: 2664786
  48. Mechanisms and regulation of lymphocyte migration.
    Immunol Today. 1989 Jan;10(1):23-8 PMID: 2665769
  49. Homing receptors and vascular addressins: cell adhesion molecules that direct lymphocyte traffic.
    Immunol Rev. 1989 Apr;108:5-18 PMID: 2670744
  50. Adhesion molecules of cultured hematopoietic malignancies. A calcium-dependent lectin is the principle mediator of binding to the high endothelial venule of lymph nodes.
    J Clin Invest. 1989 Oct;84(4):1196-205 PMID: 2794056
  51. Demonstration that a lectin-like receptor (gp90MEL) directly mediates adhesion of lymphocytes to high endothelial venules of lymph nodes.
    J Cell Biol. 1989 Nov;109(5):2463-9 PMID: 2681232
  52. Migration of lymphocytes across specialized vascular endothelium. V. Production of a sulphated macromolecule by high endothelial cells in lymph nodes.
    J Cell Sci. 1982 Oct;57:277-92 PMID: 7153260
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.113.5.1213
Published
1991-06-00
Pages
1213-21
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2289002
Subset
IM
Grants
NIGMS NIH HHS · GM23547 · United States
NIAMS NIH HHS · P60 AR20684 · United States
External Links
PubMed source DOI: 10.1083/jcb.113.5.1213 PMC Full Text View journal details
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