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PMID: 20463090 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

The Arabidopsis Paf1c complex component CDC73 participates in the modification of FLOWERING LOCUS C chromatin.

Plant physiology ·Vol. 153 ·No. 3 ·2010-07-00 ·Pages 1074-84

Yu X, Michaels SD

Abstract

FLOWERING LOCUS C (FLC) is a key repressor of flowering in Arabidopsis (Arabidopsis thaliana) and is regulated, both positively and negatively, by posttranslational histone modifications. For example, vernalization (the promotion of flowering by cold temperatures) epigenetically silences FLC expression through repressive histone modifications such as histone H3 lysine-9 dimethylation (H3K9me2) and H3K27me3. In contrast, an RNA polymerase II-associated complex (Paf1c) activates FLC expression through increased H3K4 and H3K36 methylation. As a result of this regulation, FLC has become a useful model for the study of chromatin structure in Arabidopsis. Here we show that At3g22590 is the Arabidopsis homolog of the yeast (Saccharomyces cerevisiae) Paf1c component CDC73 and is enriched at FLC chromatin. In contrast to other Paf1c component mutants that exhibit pleiotropic developmental phenotypes, the effects of cdc73 mutations are primarily limited to flowering time, suggesting that CDC73 may only be required for Paf1c function at a subset of target genes. In rapid-cycling strains, cdc73 mutants showed reduced FLC mRNA levels and decreased H3K4me3 at the FLC locus. Interestingly, in late-flowering autonomous-pathway mutants, which contain higher levels of FLC, cdc73 mutations only suppressed FLC in a subset of mutants. H3K4me3 was uniformly reduced in all autonomous-pathway cdc73 double mutants tested; however, those showing reduced FLC expression also showed an increase in H3K27me3. Thus, CDC73 is required for high levels of FLC expression in a subset of autonomous-pathway-mutant backgrounds and functions both to promote activating histone modifications (H3K4me3) as well as preventing repressive ones (e.g. H3K27me3).

MeSH Terms
Arabidopsis/genetics,growth & development,metabolism Arabidopsis Proteins/genetics,metabolism Chromatin/metabolism Chromatin Immunoprecipitation Flowers/genetics,metabolism Gene Expression Regulation, Plant Genes, Plant/genetics Genetic Loci/genetics Histones/metabolism Lysine/metabolism MADS Domain Proteins/genetics,metabolism Methylation Mutation/genetics Phenotype Sequence Homology, Amino Acid Up-Regulation/genetics
Chemicals
Arabidopsis Proteins Chromatin FLF protein, Arabidopsis FRI protein, Arabidopsis Histones MADS Domain Proteins Lysine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Yu Xuhong
Department of Biology, Indiana University, Bloomington, Indiana 47405, USA.
Michaels Scott D
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
1532-2548
DOI
10.1104/pp.110.158386
Published
2010-07-00
Epub
2010-00-12
Pages
1074-84
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC2899897
Subset
IM
Grants
NIGMS NIH HHS · R01 GM075060 · United States
NIGMS NIH HHS · 1R01GM075060-01 · United States
External Links
PubMed source DOI: 10.1104/pp.110.158386 PMC Full Text View journal details
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