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PMID: 2057356 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Tobacco nuclear gene for the 31 kd chloroplast ribonucleoprotein: genomic organization, sequence analysis and expression.

Nucleic acids research ·Vol. 19 ·No. 11 ·1991-06-11 ·Pages 2987-91

Li YQ, Ye LZ, Sugita M, Sugiura M

Abstract

We have previously identified three chloroplast ribonucleoproteins and characterized their cDNAs. Here we present the genomic organization, sequence and expression of one of their genes. The 31 kd ribonucleoprotein (cp31) from tobacco (Nicotiana sylvestris) chloroplasts is coded for by a single-copy nuclear gene. This gene was isolated and its sequence was determined. The gene contains four exons and three introns. The position of its first intron is conserved among the genes for the maize abscisic acid-induced glycine-rich protein, the human hnRNP A1 protein and cp31. The transcription start site was determined to be 168 bp upstream from the translational initiation codon in both leaf and root tissues. No alternatively spliced transcripts was detected, suggesting that a diversity of chloroplast ribonucleoproteins is generated probably by gene amplification rather than alternative splicing.

MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Southern Chloroplast Proteins Chloroplasts DNA/genetics Gene Expression Regulation Molecular Sequence Data Plant Proteins/genetics Plants, Toxic RNA Splicing RNA, Messenger/genetics Restriction Mapping Ribonucleoproteins/genetics Sequence Homology, Nucleic Acid Tobacco/genetics Transcription, Genetic
Chemicals
Chloroplast Proteins Plant Proteins RNA, Messenger Ribonucleoproteins DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Li Y Q
Center for Gene Research, Nagoya University, Japan.
Ye L Z
Sugita M
Sugiura M
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47 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.11.2987
Published
1991-06-11
Pages
2987-91
Language
English
Region
England
NLM ID
0411011
PMCID
PMC328261
Subset
IM
Databases
GENBANK
S38103, S38111, S38122, X57079, X57505, X57506, X57507, X57508, X57509, X57510
External Links
PubMed source DOI: 10.1093/nar/19.11.2987 PMC Full Text View journal details
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