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PMID: 2108127 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Complex character of senS, a novel gene regulating expression of extracellular-protein genes of Bacillus subtilis.

Journal of bacteriology ·Vol. 172 ·No. 4 ·1990-04-00 ·Pages 1939-47

Wang LF, Doi RH

Abstract

The senS gene of Bacillus subtilis, which in high copy number stimulates the expression of several extracellular-protein genes, has been cloned, genetically mapped, and sequenced. The gene codes for a highly charged basic protein containing 65 amino acid residues. The gene is characterized by the presence of a transcription terminator (attenuator) located between the promoter and open reading frame, a strong ribosome-binding site, and a strong transcription terminator at the 3' end of this monocistronic gene. The amino acid sequence of SenS showed partial homology with the N-terminal core binding domain region of bacterial RNA polymerase sigma factors and a helix-turn-helix motif found in DNA-binding proteins. The gene can be deleted without any effect on growth or sporulation.

MeSH Terms
Amino Acid Sequence Bacillus subtilis/genetics Bacterial Proteins/genetics Base Sequence Cloning, Molecular Escherichia coli/genetics Gene Conversion Gene Expression Regulation, Bacterial Genes, Bacterial Molecular Sequence Data Plasmids Restriction Mapping Sequence Homology, Nucleic Acid Transcription Factors Transduction, Genetic
Chemicals
Bacterial Proteins Transcription Factors senS protein, Bacillus subtilis
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wang L F
Department of Biochemistry and Biophysics, University of California, Davis 95616.
Doi R H
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45 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.4.1939-1947.1990
Published
1990-04-00
Pages
1939-47
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC208689
Subset
IM
Grants
NIGMS NIH HHS · GM19673 · United States
Databases
GENBANK
M34826
External Links
PubMed source DOI: 10.1128/jb.172.4.1939-1947.1990 PMC Full Text View journal details
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