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PMID: 2117437 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The fruitfly Drosophila melanogaster contains a novel charged adipokinetic-hormone-family peptide.

The Biochemical journal ·Vol. 269 ·No. 2 ·1990-07-15 ·Pages 315-20

Schaffer MH, Noyes BE, Slaughter CA, Thorne GC, Gaskell SJ

Abstract

A member of the RPCH/AKH (red-pigment-concentrating hormone/adipokinetic hormone) family of arthropod neuropeptides was identified in the fruitfly Drosophila melanogaster, and its structure was determined by automated Edman degradation and m.s. using fast-atom-bombardment ionization and a tandem hybrid instrument capable of high sensitivity. The sequence of this peptide, which we call 'DAKH', is pGlu-Leu-Thr-Phe-Ser-Pro-Asp-Trp-NH2 (where pGlu is pyroglutamic acid and Trp-NH2 is tryptophan carboxyamide). H.p.l.c. analyses of extracts of the three body segments revealed that more than 80% of the peptide is contained in the thorax. Although DAKH is typical of family members in its general structure and distribution in the animal, it is unique in containing a residue which is charged under physiological conditions. The evolutionary significance of this change is considered.

MeSH Terms
Amino Acid Sequence Animals Biological Assay Chromatography, High Pressure Liquid Drosophila melanogaster/analysis Grasshoppers Insect Hormones/isolation & purification,pharmacology Mass Spectrometry Molecular Sequence Data Muscle Contraction/drug effects Oligopeptides/isolation & purification,pharmacology Pyrrolidonecarboxylic Acid/analogs & derivatives
Chemicals
Insect Hormones Oligopeptides adipokinetic hormone DAKH peptide red pigment-concentrating hormone Pyrrolidonecarboxylic Acid
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Schaffer M H
Department of Psychiatry, University of Texas Southwestern Medical Center, Dallas 75235-9070.
Noyes B E
Slaughter C A
Thorne G C
Gaskell S J
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2690315
Published
1990-07-15
Pages
315-20
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1131578
Subset
IM
Grants
NIAID NIH HHS · AI 26920 · United States
NIGMS NIH HHS · GM 34120 · United States
External Links
PubMed source DOI: 10.1042/bj2690315 PMC Full Text View journal details
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