Home LiteratureArticle Details
PMID: 2147224 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The yeast PRP6 gene encodes a U4/U6 small nuclear ribonucleoprotein particle (snRNP) protein, and the PRP9 gene encodes a protein required for U2 snRNP binding.

Molecular and cellular biology ·Vol. 10 ·No. 12 ·1990-12-00 ·Pages 6417-25

Abovich N, Legrain P, Rosbash M

Abstract

PRP6 and PRP9 are two yeast genes involved in pre-mRNA splicing. Incubation at 37 degrees C of strains that carry temperature-sensitive mutations at these loci inhibits splicing, and in vivo experiments suggested that they might be involved in commitment complex formation (P. Legrain and M. Rosbash, Cell 57:573-583, 1989). To examine the specific role that the PRP6 and PRP9 products may play in splicing or pre-mRNA transport to the cytoplasm, we have characterized in vitro splicing and spliceosome assembly in extracts derived from prp6 and prp9 mutant strains. We have also characterized RNAs that are specifically immunoprecipitated with the PRP6 and PRP9 proteins. Both approaches indicate that PRP6 encodes a U4/U6 small nuclear ribonucleoprotein particle (snRNP) protein and that the PRP9 protein is required for a stable U2 snRNP-substrate interaction. The results are discussed with reference to the previously observed in vivo phenotypes of these mutants.

Related Genes
PRP6 PRP9
MeSH Terms
Genes, Fungal Genetic Complementation Test Protein Binding RNA Precursors/genetics RNA Splicing RNA, Small Nuclear/genetics Ribonucleoproteins/genetics,metabolism Ribonucleoproteins, Small Nuclear Saccharomyces cerevisiae/genetics,metabolism Transcription, Genetic
Chemicals
RNA Precursors RNA, Small Nuclear Ribonucleoproteins Ribonucleoproteins, Small Nuclear
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Abovich N
Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 02254.
Legrain P
Rosbash M
References (35)
35 references, click to expand
  1. Identification of ten genes that control ribosome formation in yeast.
    Mol Gen Genet. 1970;109(1):42-56 PMID: 5488085
  2. The molecular characterization of PRP6 and PRP9 yeast genes reveals a new cysteine/histidine motif common to several splicing factors.
    EMBO J. 1990 Sep;9(9):2775-81 PMID: 2118103
  3. Transformation of intact yeast cells treated with alkali cations.
    J Bacteriol. 1983 Jan;153(1):163-8 PMID: 6336730
  4. The structure of an antigenic determinant in a protein.
    Cell. 1984 Jul;37(3):767-78 PMID: 6204768
  5. Rapid and efficient site-specific mutagenesis without phenotypic selection.
    Proc Natl Acad Sci U S A. 1985 Jan;82(2):488-92 PMID: 3881765
  6. Molecular consequences of specific intron mutations on yeast mRNA splicing in vivo and in vitro.
    Cell. 1985 Aug;42(1):335-44 PMID: 3893746
  7. The yeast RNA gene products are essential for mRNA splicing in vitro.
    Cell. 1986 Dec 26;47(6):953-63 PMID: 3536128
  8. Electrophoresis of ribonucleoproteins reveals an ordered assembly pathway of yeast splicing complexes.
    Nature. 1986 Nov 27-Dec 3;324(6095):341-5 PMID: 3537805
  9. Pre-mRNA splicing.
    Annu Rev Genet. 1986;20:671-708 PMID: 2880558
  10. Interactions between small nuclear ribonucleoprotein particles in formation of spliceosomes.
    Cell. 1987 Jun 19;49(6):763-74 PMID: 2953438
  11. S. cerevisiae U1 RNA is large and has limited primary sequence homology to metazoan U1 snRNA.
    Cell. 1987 Aug 14;50(4):593-602 PMID: 2440584
  12. An ordered pathway of snRNP binding during mammalian pre-mRNA splicing complex assembly.
    EMBO J. 1987 Aug;6(8):2415-24 PMID: 2959470
  13. A novel role for the 3' region of introns in pre-mRNA splicing of Saccharomyces cerevisiae.
    Genes Dev. 1987 May;1(3):238-46 PMID: 3315850
  14. Identification of a yeast snRNP protein and detection of snRNP-snRNP interactions.
    Cell. 1987 Dec 24;51(6):1019-26 PMID: 2961458
  15. Spliceosome assembly in yeast.
    Genes Dev. 1987 Nov;1(9):1014-27 PMID: 2962902
  16. Spliceosome assembly involves the binding and release of U4 small nuclear ribonucleoprotein.
    Proc Natl Acad Sci U S A. 1988 Jan;85(2):411-5 PMID: 2963332
  17. A factor, U2AF, is required for U2 snRNP binding and splicing complex assembly.
    Cell. 1988 Jan 29;52(2):207-19 PMID: 2963698
  18. A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector.
    Gene. 1987;60(2-3):237-43 PMID: 3327750
  19. Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method.
    Mol Cell Biol. 1988 May;8(5):2159-65 PMID: 2455217
  20. Different small nuclear ribonucleoprotein particles are involved in different steps of splicing complex formation.
    Cold Spring Harb Symp Quant Biol. 1987;52:287-98 PMID: 2969789
  21. RNA11 protein is associated with the yeast spliceosome and is localized in the periphery of the cell nucleus.
    Mol Cell Biol. 1988 Jun;8(6):2379-93 PMID: 3043176
  22. A U1 snRNA:pre-mRNA base pairing interaction is required early in yeast spliceosome assembly but does not uniquely define the 5' cleavage site.
    EMBO J. 1988 Aug;7(8):2533-8 PMID: 3056718
  23. An early hierarchic role of U1 small nuclear ribonucleoprotein in spliceosome assembly.
    Science. 1988 Nov 18;242(4881):1028-35 PMID: 2973660
  24. Early commitment of yeast pre-mRNA to the spliceosome pathway.
    Mol Cell Biol. 1988 Sep;8(9):3755-60 PMID: 3065622
  25. Spliceosomal snRNAs.
    Annu Rev Genet. 1988;22:387-419 PMID: 2977088
  26. Some cis- and trans-acting mutants for splicing target pre-mRNA to the cytoplasm.
    Cell. 1989 May 19;57(4):573-83 PMID: 2655924
  27. PRP4 (RNA4) from Saccharomyces cerevisiae: its gene product is associated with the U4/U6 small nuclear ribonucleoprotein particle.
    Mol Cell Biol. 1989 Sep;9(9):3698-709 PMID: 2528686
  28. PRP4: a protein of the yeast U4/U6 small nuclear ribonucleoprotein particle.
    Mol Cell Biol. 1989 Sep;9(9):3710-9 PMID: 2528687
  29. Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiae.
    Genes Dev. 1989 Aug;3(8):1206-16 PMID: 2676722
  30. Identification of functional U1 snRNA-pre-mRNA complexes committed to spliceosome assembly and splicing.
    Cell. 1989 Oct 20;59(2):349-58 PMID: 2529976
  31. Identification, purification, and biochemical characterization of U2 small nuclear ribonucleoprotein auxiliary factor.
    Proc Natl Acad Sci U S A. 1989 Dec;86(23):9243-7 PMID: 2531895
  32. Nuclear pre-mRNA splicing in yeast.
    Yeast. 1989 Nov-Dec;5(6):439-57 PMID: 2694677
  33. The PRP4 (RNA4) protein of Saccharomyces cerevisiae is associated with the 5' portion of the U4 small nuclear RNA.
    Mol Cell Biol. 1990 Mar;10(3):1217-25 PMID: 2154681
  34. The NUP1 gene encodes an essential component of the yeast nuclear pore complex.
    Cell. 1990 Jun 15;61(6):965-78 PMID: 2190694
  35. The effect of temperature-sensitive RNA mutants on the transcription products from cloned ribosomal protein genes of yeast.
    Cell. 1981 Jun;24(3):679-86 PMID: 7018694
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.12.6417-6425.1990
Published
1990-12-00
Pages
6417-25
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362918
Subset
IM
Grants
NIGMS NIH HHS · GM23549 · United States
External Links
PubMed source DOI: 10.1128/mcb.10.12.6417-6425.1990 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2