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PMID: 21487094 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Arabidopsis ABERRANT PEROXISOME MORPHOLOGY9 is a peroxin that recruits the PEX1-PEX6 complex to peroxisomes.

The Plant cell ·Vol. 23 ·No. 4 ·2011-04-00 ·Pages 1573-87

Goto S, Mano S, Nakamori C, Nishimura M

Abstract

Peroxisomes have pivotal roles in several metabolic processes, such as the detoxification of H₂O₂ and β-oxidation of fatty acids, and their functions are tightly regulated by multiple factors involved in peroxisome biogenesis, including protein transport. This study describes the isolation of an embryonic lethal Arabidopsis thaliana mutant, aberrant peroxisome morphology9 (apem9), which is compromised in protein transport into peroxisomes. The APEM9 gene was found to encode an unknown protein. Compared with apem9 having the nucleotide substitution, the knockdown mutants showed severe defects in peroxisomal functions and plant growth. We showed that expression of APEM9 altered PEROXIN6 (PEX6) subcellular localization from the cytosol to peroxisomes. In addition, we showed that PEX1 and PEX6 comprise a heterooligomer and that this complex was recruited to peroxisomal membranes via protein-protein interactions of APEM9 with PEX6. These findings show that APEM9 functions as an anchoring protein, similar to Pex26 in mammals and Pex15p in yeast. Interestingly, however, the identities of amino acids among these anchoring proteins are quite low. These results indicate that although the association of the PEX1-PEX6 complex with peroxisomal membranes is essential for peroxisomal functions, the protein that anchors this complex evolved uniquely in plants.

MeSH Terms
ATPases Associated with Diverse Cellular Activities Adenosine Triphosphatases/metabolism Amino Acid Sequence Arabidopsis/chemistry,genetics,metabolism Arabidopsis Proteins/genetics,metabolism DNA, Bacterial/genetics Gene Expression Regulation, Plant Gene Knockdown Techniques Genes, Plant/genetics Intracellular Membranes/metabolism Membrane Proteins/genetics,metabolism Molecular Sequence Data Mutagenesis, Insertional/genetics Mutant Proteins/isolation & purification Mutation/genetics Organ Specificity/genetics Peroxisomes/metabolism Phenotype Protein Interaction Mapping Protein Transport Saccharomyces cerevisiae/cytology,metabolism Seeds/growth & development
Chemicals
APEM9 protein, Arabidopsis Arabidopsis Proteins DNA, Bacterial Membrane Proteins Mutant Proteins T-DNA Adenosine Triphosphatases ATPases Associated with Diverse Cellular Activities PEROXIN6 protein, Arabidopsis PEX1 protein, Arabidopsis
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Goto Shino
Department of Cell Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan.
Mano Shoji
Nakamori Chihiro
Nishimura Mikio
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1532-298X
DOI
10.1105/tpc.110.080770
Published
2011-04-00
Epub
2011-00-12
Pages
1573-87
Language
English
Region
England
NLM ID
9208688
PMCID
PMC3101541
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.110.080770 PMC Full Text View journal details
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