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PMID: 2158270 Published · ppublish English Journal Article Review

In vitro assays used to measure the activity of topoisomerases.

Antimicrobial agents and chemotherapy ·Vol. 34 ·No. 1 ·1990-01-00 ·Pages 1-7

Barrett JF, Sutcliffe JA, Gootz TD

Abstract

暂无摘要

MeSH Terms
Bacteria/enzymology,metabolism DNA Topoisomerases, Type I/analysis,metabolism
Chemicals
DNA Topoisomerases, Type I
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Barrett J F
Department of Immunology and Infectious Diseases, Pfizer Central Research, Groton, Connecticut 06340.
Sutcliffe J A
Gootz T D
References (96)
96 references, click to expand
  1. Potent catenation of supercoiled and gapped DNA circles by topoisomerase I in the presence of a hydrophilic polymer.
    J Biol Chem. 1984 Apr 10;259(7):4576-81 PMID: 6323479
  2. Mechanism of antitumor drug action: poisoning of mammalian DNA topoisomerase II on DNA by 4'-(9-acridinylamino)-methanesulfon-m-anisidide.
    Proc Natl Acad Sci U S A. 1984 Mar;81(5):1361-5 PMID: 6324188
  3. Rapid detection and isolation of covalent DNA/protein complexes: application to topoisomerase I and II.
    EMBO J. 1984 Mar;3(3):671-6 PMID: 6325181
  4. HMG17 protein facilitates the DNA catenation reaction catalyzed by DNA topoisomerases.
    Arch Biochem Biophys. 1984 May 15;231(1):169-74 PMID: 6326673
  5. Inhibition of the DNA catenation activity of type II topoisomerase by VP16-213 and VM26.
    Biochem Biophys Res Commun. 1984 Jul 18;122(1):165-70 PMID: 6331440
  6. Nonintercalative antitumor drugs interfere with the breakage-reunion reaction of mammalian DNA topoisomerase II.
    J Biol Chem. 1984 Nov 10;259(21):13560-6 PMID: 6092381
  7. Biology of bacterial deoxyribonucleic acid topoisomerases.
    Microbiol Rev. 1984 Dec;48(4):273-89 PMID: 6097803
  8. Mechanism of inhibition of DNA gyrase by analogues of nalidixic acid: the target of the drugs is DNA.
    Proc Natl Acad Sci U S A. 1985 Jan;82(2):307-11 PMID: 2982149
  9. A type-II DNA topoisomerase and a catenating protein from the transplantable VX2 carcinoma.
    Eur J Biochem. 1985 Feb 1;146(3):483-8 PMID: 2982598
  10. Purification and characterization of a type II DNA topoisomerase from bovine calf thymus.
    J Biol Chem. 1985 Feb 25;260(4):2475-82 PMID: 2982821
  11. Novel partitioning of DNA cleavage sites for Drosophila topoisomerase II.
    Cell. 1985 Apr;40(4):933-41 PMID: 2985270
  12. Duplex DNA knots produced by Escherichia coli topoisomerase I. Structure and requirements for formation.
    J Biol Chem. 1985 Apr 25;260(8):4975-83 PMID: 2985571
  13. Mechanism of strand passage by Escherichia coli topoisomerase I. The role of the required nick in catenation and knotting of duplex DNA.
    J Biol Chem. 1985 Apr 25;260(8):4984-94 PMID: 2985572
  14. Purification and characterization of Plasmodium berghei DNA topoisomerases I and II: drug action, inhibition of decatenation and relaxation, and stimulation of DNA cleavage.
    Biochemistry. 1986 Apr 8;25(7):1471-9 PMID: 3011062
  15. Inhibitors of trypanosome topoisomerases.
    Biochem Soc Trans. 1986 Apr;14(2):496-9 PMID: 3011554
  16. Quantitation of single- and double-strand DNA breaks in vitro and in vivo.
    Anal Biochem. 1986 May 1;154(2):485-91 PMID: 3524311
  17. Effect of 4-quinolones and novobiocin on calf thymus DNA polymerase alpha primase complex, topoisomerases I and II, and growth of mammalian lymphoblasts.
    Antimicrob Agents Chemother. 1986 Jun;29(6):1073-8 PMID: 3015015
  18. DNA gyrase complex with DNA: determinants for site-specific DNA breakage.
    EMBO J. 1986 Jun;5(6):1411-8 PMID: 3015604
  19. A quantitative decatenation assay for type II topoisomerases.
    Anal Biochem. 1986 Aug 1;156(2):364-79 PMID: 3021018
  20. New structure-activity relationships of the quinolone antibacterials using the target enzyme. The development and application of a DNA gyrase assay.
    J Med Chem. 1986 Mar;29(3):394-404 PMID: 3005575
  21. Mechanism of inhibition of DNA gyrase by quinolone antibacterials: a cooperative drug--DNA binding model.
    Biochemistry. 1989 May 2;28(9):3886-94 PMID: 2546585
  22. Biochemical characteristics and physiological significance of major DNA topoisomerases.
    Antimicrob Agents Chemother. 1989 Dec;33(12):2027-33 PMID: 2559654
  23. Inhibitory effects of quinolone antibacterial agents on eucaryotic topoisomerases and related test systems.
    Antimicrob Agents Chemother. 1990 Jan;34(1):8-12 PMID: 2158274
  24. Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels.
    Biochemistry. 1968 Feb;7(2):668-74 PMID: 4868544
  25. Interaction between DNA and an Escherichia coli protein omega.
    J Mol Biol. 1971 Feb 14;55(3):523-33 PMID: 4927945
  26. Intercalative antitumor drugs interfere with the breakage-reunion reaction of mammalian DNA topoisomerase II.
    J Biol Chem. 1984 Jul 25;259(14):9182-7 PMID: 6086625
  27. In vivo mapping of DNA topoisomerase II-specific cleavage sites on SV40 chromatin.
    Cell. 1985 May;41(1):127-32 PMID: 2986841
  28. DNA topoisomerases: enzymes that control DNA conformation.
    Curr Top Microbiol Immunol. 1985;114:19-102 PMID: 2986908
  29. Mapping of DNA gyrase cleavage sites in vivo oxolinic acid induced cleavages in plasmid pBR322.
    J Mol Biol. 1985 Feb 20;181(4):545-50 PMID: 2987505
  30. Replication of pBR322 DNA in vitro with purified proteins. Requirement for topoisomerase I in the maintenance of template specificity.
    J Biol Chem. 1985 Aug 5;260(16):9316-25 PMID: 2991240
  31. DNA topoisomerases.
    Annu Rev Biochem. 1985;54:665-97 PMID: 2992360
  32. Effects of the DNA intercalators 4'-(9-acridinylamino)methanesulfon-m-anisidide and 2-methyl-9-hydroxyellipticinium on topoisomerase II mediated DNA strand cleavage and strand passage.
    Biochemistry. 1985 Nov 5;24(23):6410-6 PMID: 3002440
  33. Purification and characterization of DNA topoisomerase II from calf thymus associated with polypeptides of 175 and 150 kDa.
    Eur J Biochem. 1986 Nov 3;160(3):451-7 PMID: 3023077
  34. In vivo localization of DNA topoisomerase II cleavage sites on Drosophila heat shock chromatin.
    Mol Cell Biol. 1986 Apr;6(4):985-92 PMID: 3023886
  35. Mechanistic aspects of DNA topoisomerases.
    Adv Protein Chem. 1986;38:69-107 PMID: 3026152
  36. Mapping the active site tyrosine of Escherichia coli DNA gyrase.
    J Biol Chem. 1987 Apr 15;262(11):5339-44 PMID: 3031051
  37. Effects of ciprofloxacin on eucaryotic pyrimidine nucleotide biosynthesis and cell growth.
    Antimicrob Agents Chemother. 1987 May;31(5):774-9 PMID: 3606077
  38. DNA gyrase-catalyzed decatenation of multiply linked DNA dimers.
    J Biol Chem. 1987 Jul 25;262(21):10362-8 PMID: 3038875
  39. Calcium-promoted DNA cleavage by eukaryotic topoisomerase II: trapping the covalent enzyme-DNA complex in an active form.
    Biochemistry. 1987 Jul 14;26(14):4303-9 PMID: 2822084
  40. Major discrepancies between results obtained with two different methods for evaluating DNA damage: alkaline elution and alkaline unwinding. Possible explanations.
    Anticancer Res. 1987 Jul-Aug;7(4B):669-80 PMID: 3674758
  41. Purification of topoisomerase II from amsacrine-resistant P388 leukemia cells. Evidence for two forms of the enzyme.
    J Biol Chem. 1987 Dec 5;262(34):16739-47 PMID: 2824504
  42. Effect of DNA gyrase inhibitors pefloxacin, five other quinolones, novobiocin, and clorobiocin on Escherichia coli topoisomerase I.
    Antimicrob Agents Chemother. 1987 Dec;31(12):1925-8 PMID: 2830840
  43. Mode of action of the quinolone antimicrobial agents.
    Rev Infect Dis. 1988 Jan-Feb;10 Suppl 1:S14-21 PMID: 2831608
  44. Studies on the interaction of 4-quinolones with DNA by DNA unwinding experiments.
    Biochim Biophys Acta. 1988 Mar 31;949(3):279-87 PMID: 2831987
  45. Inhibitors of DNA topoisomerases.
    Biochemistry. 1988 Apr 5;27(7):2253-9 PMID: 2838070
  46. In vitro cleavable-complex assay to monitor antimicrobial potency of quinolones.
    Antimicrob Agents Chemother. 1988 Jul;32(7):1086-9 PMID: 2847641
  47. DNA gyrase binds to the family of prokaryotic repetitive extragenic palindromic sequences.
    Proc Natl Acad Sci U S A. 1988 Dec;85(23):8850-4 PMID: 2848243
  48. Mechanism of quinolone inhibition of DNA gyrase. Appearance of unique norfloxacin binding sites in enzyme-DNA complexes.
    J Biol Chem. 1989 Feb 15;264(5):2973-8 PMID: 2536729
  49. Genetic profile of a nalidixic acid analog: a model for the mechanism of sister chromatid exchange induction.
    Environ Mol Mutagen. 1989;13(3):238-52 PMID: 2539998
  50. Biochemical basis for the interactions of type I and type II topoisomerases with DNA.
    Pharmacol Ther. 1989;41(1-2):223-41 PMID: 2540496
  51. DNA topoisomerases.
    Gene Amplif Anal. 1981;2:455-73 PMID: 6101057
  52. Cloning and simplified purification of Escherichia coli DNA gyrase A and B proteins.
    J Biol Chem. 1984 Jul 25;259(14):9199-201 PMID: 6086626
  53. DNA gyrase: an enzyme that introduces superhelical turns into DNA.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):3872-6 PMID: 186775
  54. Strand breakage by the DNA untwisting enzyme results in covalent attachment of the enzyme to DNA.
    Proc Natl Acad Sci U S A. 1977 Sep;74(9):3800-4 PMID: 198805
  55. Mechanism of action of nalidixic acid: purification of Escherichia coli nalA gene product and its relationship to DNA gyrase and a novel nicking-closing enzyme.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):4767-71 PMID: 200930
  56. Nalidixic acid resistance: a second genetic character involved in DNA gyrase activity.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):4772-6 PMID: 337300
  57. Energy coupling in DNA gyrase and the mechanism of action of novobiocin.
    Proc Natl Acad Sci U S A. 1978 Oct;75(10):4838-42 PMID: 368801
  58. Site-specific cleavage of DNA by E. coli DNA gyrase.
    Cell. 1979 May;17(1):175-84 PMID: 378403
  59. Structure and activities of Escherichia coli DNA gyrase.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:41-52 PMID: 225110
  60. Nucleotide sequence of small ColE1 derivatives: structure of the regions essential for autonomous replication and colicin E1 immunity.
    Mol Gen Genet. 1979 May 4;172(2):151-9 PMID: 384144
  61. DNA gyrase on the bacterial chromosome: DNA cleavage induced by oxolinic acid.
    J Mol Biol. 1979 Jun 25;131(2):287-302 PMID: 226717
  62. A sign inversion mechanism for enzymatic supercoiling of DNA.
    Science. 1979 Nov 30;206(4422):1081-3 PMID: 227059
  63. A topoisomerase from Escherichia coli related to DNA gyrase.
    Proc Natl Acad Sci U S A. 1979 Dec;76(12):6110-4 PMID: 230498
  64. Interaction between DNA gyrase and its cleavage site on DNA.
    J Biol Chem. 1980 Mar 10;255(5):2211-9 PMID: 6243661
  65. Type II DNA topoisomerases: enzymes that can unknot a topologically knotted DNA molecule via a reversible double-strand break.
    Cell. 1980 Mar;19(3):697-707 PMID: 6244895
  66. DNA gyrase action involves the introduction of transient double-strand breaks into DNA.
    Proc Natl Acad Sci U S A. 1980 Apr;77(4):1847-51 PMID: 6246508
  67. Covalent bonds between protein and DNA. Formation of phosphotyrosine linkage between certain DNA topoisomerases and DNA.
    J Biol Chem. 1980 Jun 25;255(12):5560-5 PMID: 6155377
  68. Formation and resolution of DNA catenanes by DNA gyrase.
    Cell. 1980 May;20(1):245-54 PMID: 6248235
  69. ATP-dependent DNA topoisonmerase from D. melanogaster reversibly catenates duplex DNA rings.
    Cell. 1980 Aug;21(1):115-25 PMID: 6250707
  70. DNA gyrase subunit stoichiometry and the covalent attachment of subunit A to DNA during DNA cleavage.
    Nucleic Acids Res. 1980 Sep 11;8(17):3865-74 PMID: 6255421
  71. Fluorometric method for rapid detection of DNA strand breaks in human white blood cells produced by low doses of radiation.
    Cancer Res. 1981 May;41(5):1889-92 PMID: 7214357
  72. Mapping the topography of DNA wrapped around gyrase by nucleolytic and chemical probing of complexes of unique DNA sequences.
    Cell. 1981 Mar;23(3):721-9 PMID: 6261954
  73. Catenation and knotting of duplex DNA by type 1 topoisomerases: a mechanistic parallel with type 2 topoisomerases.
    Proc Natl Acad Sci U S A. 1981 Feb;78(2):843-7 PMID: 6262776
  74. A homogeneous type II DNA topoisomerase from HeLa cell nuclei.
    J Biol Chem. 1981 Sep 10;256(17):9334-9 PMID: 6267071
  75. DNA topoisomerases.
    Annu Rev Biochem. 1981;50:879-910 PMID: 6267993
  76. DNA gyrase: affinity chromatography on novobiocin-Sepharose and catalytic properties.
    Nucleic Acids Res. 1981 Aug 11;9(15):3589-603 PMID: 6269086
  77. Site-specific interaction of DNA gyrase with DNA.
    Proc Natl Acad Sci U S A. 1981 Jul;78(7):4165-9 PMID: 6270661
  78. DNA gyrase: site-specific interactions and transient double-strand breakage of DNA.
    Cold Spring Harb Symp Quant Biol. 1981;45 Pt 1:391-8 PMID: 6271484
  79. Novel topologically knotted DNA from bacteriophage P4 capsids: studies with DNA topoisomerases.
    Nucleic Acids Res. 1981 Aug 25;9(16):3979-89 PMID: 6272191
  80. Catenation of DNA rings by topoisomerases. Mechanism of control by spermidine.
    J Biol Chem. 1982 Mar 10;257(5):2687-93 PMID: 6277910
  81. Intra- and intermolecular strand transfer by HeLa DNA topoisomerase I.
    J Biol Chem. 1982 Apr 10;257(7):3995-4000 PMID: 6277956
  82. DNA topoisomerases from rat liver: physiological variations.
    Nucleic Acids Res. 1983 Feb 25;11(4):1059-75 PMID: 6298730
  83. Positively supercoiled plasmid DNA is produced by treatment of Escherichia coli with DNA gyrase inhibitors.
    Nucleic Acids Res. 1983 May 25;11(10):2999-3017 PMID: 6304617
  84. Escherichia coli type-1 topoisomerases: identification, mechanism, and role in recombination.
    Cold Spring Harb Symp Quant Biol. 1983;47 Pt 2:769-77 PMID: 6305585
  85. Knotting of the circular duplex DNA by type II DNA topoisomerase from Drosophila melanogaster.
    J Biol Chem. 1983 Jul 10;258(13):8413-20 PMID: 6305983
  86. Double strand DNA cleavage by type II DNA topoisomerase from Drosophila melanogaster.
    J Biol Chem. 1983 Jul 10;258(13):8421-8 PMID: 6305984
  87. Reversible decatenation of kinetoplast DNA by a DNA topoisomerase from trypanosomatids.
    Nucleic Acids Res. 1983 Jun 25;11(12):4019-34 PMID: 6306572
  88. DNA topoisomerase II from Drosophila melanogaster. Purification and physical characterization.
    J Biol Chem. 1983 Aug 10;258(15):9530-5 PMID: 6308010
  89. DNA topoisomerase II from Drosophila melanogaster. Relaxation of supercoiled DNA.
    J Biol Chem. 1983 Aug 10;258(15):9536-43 PMID: 6308011
  90. Nucleosomes contain DNA binding proteins that resist dissociation by sodium dodecyl sulfate.
    Biochem Biophys Res Commun. 1983 Jul 18;114(1):99-106 PMID: 6309176
  91. Purification and properties of type II DNA topoisomerase from embryos of Drosophila melanogaster.
    Methods Enzymol. 1983;100:161-70 PMID: 6312257
  92. Escherichia coli DNA gyrase.
    Methods Enzymol. 1983;100:171-80 PMID: 6312258
  93. Cleavage of DNA by mammalian DNA topoisomerase II.
    J Biol Chem. 1983 Dec 25;258(24):15365-70 PMID: 6317692
  94. DNA topoisomerases--enzymes that catalyse the breaking and rejoining of DNA.
    CRC Crit Rev Biochem. 1983;15(1):1-24 PMID: 6319080
  95. Yeast DNA topoisomerase II is encoded by a single-copy, essential gene.
    Cell. 1984 Apr;36(4):1073-80 PMID: 6323017
  96. Inhibition of the reactions catalysed by a type I topoisomerase and catenating enzyme of Trypanosoma cruzi by DNA-intercalating drugs. Preferential inhibition of the catenating reaction.
    EMBO J. 1984 Jan;3(1):11-6 PMID: 6323159
Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.34.1.1
Published
1990-01-00
Pages
1-7
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC171509
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.34.1.1 PMC Full Text View journal details
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