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PMID: 2162903 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Molecular analysis of the influences of positive selection, tolerance induction, and antigen presentation on the T cell receptor repertoire.

The Journal of experimental medicine ·Vol. 172 ·No. 1 ·1990-07-01 ·Pages 139-50

Fink PJ, Blair MJ, Matis LA, Hedrick SM

Abstract

Immunization of both B10.A and B10.S(9R) mice with pigeon cytochrome c (pcc) elicits T cells capable of proliferating to pcc presented on I-E major histocompatibility complex (MHC) molecules. The T cell receptor (TCR) repertoire used by pcc-specific T cells from these two strains is markedly different, even for T cells recognizing very similar antigen/MHC complexes. Our current studies have been directed toward explaining this differential expression between MHC congenic strains of TCR gene elements capable of recognizing similar ligands. Analysis of the TCR repertoire of pcc-specific T cells from F1[B10.A x B10.S (9R)]----parent radiation chimeras has demonstrated that much of this difference is a result of the positive selection of T cells for MHC restriction specificity. Further analysis of T cell lines from F1 mice and from radiation chimeras stimulated in vitro with pcc on both B10.A and B10.S(9R) antigen-presenting cells has provided clear-cut examples of the influence of positive selection, tolerance induction and of both in vivo and in vitro antigen presentation on the shaping of the TCR repertoire for a protein antigen. This is the first molecular analysis of how positive selection, tolerance induction, and antigen presentation can combine to mold the TCR repertoire.

MeSH Terms
Animals Antigen-Presenting Cells/immunology Blotting, Southern Cell Line Chimera/immunology Columbidae Cytochrome c Group/immunology Female Gene Rearrangement/genetics Immune Tolerance/immunology Immunization Lymphocyte Activation/immunology Major Histocompatibility Complex Male Mice Mice, Inbred Strains Phenotype Receptors, Antigen, T-Cell/genetics,immunology Selection, Genetic T-Lymphocytes/immunology
Chemicals
Cytochrome c Group Receptors, Antigen, T-Cell
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Fink P J
Department of Immunology, Research Institute of Scripps Clinic, La Jolla, California 92037.
Blair M J
Matis L A
Hedrick S M
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.172.1.139
Published
1990-07-01
Pages
139-50
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2188180
Subset
IM
Grants
PHS HHS · A11372 · United States
PHS HHS · A127417 · United States
NIGMS NIH HHS · GM-35880 · United States
External Links
PubMed source DOI: 10.1084/jem.172.1.139 PMC Full Text View journal details
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