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PMID: 2165478 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Overexpression of virD1 and virD2 genes in Agrobacterium tumefaciens enhances T-complex formation and plant transformation.

Journal of bacteriology ·Vol. 172 ·No. 8 ·1990-08-00 ·Pages 4432-40

Wang K, Herrera-Estrella A, Van Montagu M

Abstract

The VirD1 and VirD2 proteins encoded by an inducible locus of the virulence (vir) region of the Agrobacterium tumefaciens Ti plasmid are required for site-specific nicking at T-DNA border sites. We have determined the nucleotide sequence of a 3.6-kilobase-pair fragment carrying the virD locus from nopaline Ti plasmid pTiC58. In contrast to the previous report (Hagiya et al., Proc. Natl. Acad. Sci. USA 82:2669-2673, 1985), we found that the first three open reading frames were capable of encoding polypeptides of 16.1, 49.7, and 21.4 kilodaltons. Deletion analysis demonstrated that the N-terminal conserved domain of VirD2 was absolutely essential for its endonuclease activity. When extra copies of the virD1 and virD2 genes were present in an A. tumefaciens strain carrying a Ti plasmid, increased amounts of T-strand and nicked molecules could be detected at early stages of vir induction. Such strains possessed the ability to transform plants with higher efficiency.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Escherichia coli/genetics Gene Expression Genes, Bacterial Kanamycin Kinase Molecular Sequence Data Phosphotransferases/genetics Plants/genetics,microbiology Plasmids Protein Conformation Restriction Mapping Rhizobium/genetics,pathogenicity Sequence Homology, Nucleic Acid Transformation, Genetic Virulence/genetics Virulence Factors
Chemicals
Bacterial Proteins Virulence Factors Phosphotransferases Kanamycin Kinase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wang K
Laboratorium voor Genetica, Rijksuniversiteit Gent, Belgium.
Herrera-Estrella A
Van Montagu M
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40 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.8.4432-4440.1990
Published
1990-08-00
Pages
4432-40
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC213272
Subset
IM
Databases
GENBANK
M33673
External Links
PubMed source DOI: 10.1128/jb.172.8.4432-4440.1990 PMC Full Text View journal details
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