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PMID: 2167438 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Characterization and cDNA cloning of phospholipase C-gamma, a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.

Molecular and cellular biology ·Vol. 10 ·No. 9 ·1990-09-00 ·Pages 4770-7

Burgess WH, Dionne CA, Kaplow J, Mudd R, Friesel R, Zilberstein A, Schlessinger J, Jaye M

Abstract

Heparin-binding growth factors (HBGFs) bind to high-affinity cell surface receptors which possess intrinsic tyrosine kinase activity. A Mr 150,000 protein phosphorylated on tyrosine in response to class 1 HBGF (HBGF-1) was purified and partially sequenced. On the basis of this sequence, cDNA clones were isolated from a human endothelial cell library and identified as encoding phospholipase C-gamma. Phosphorylation of phospholipase C-gamma in intact cells treated with HBGF-1 was directly demonstrated by using antiphospholipase C-gamma antibodies. Thus, HBGF-1 joins epidermal growth factor and platelet-derived growth factor, whose receptor activation leads to tyrosine phosphorylation and probable activation of phospholipase C-gamma.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cells, Cultured Cloning, Molecular DNA/genetics,isolation & purification Enzyme Activation Fibroblast Growth Factors/pharmacology Gene Library Humans Isoenzymes/genetics,metabolism Mice Mice, Inbred Strains Molecular Sequence Data Oligonucleotide Probes Phosphorylation Protein-Tyrosine Kinases/metabolism Receptors, Cell Surface/metabolism Receptors, Fibroblast Growth Factor Substrate Specificity Type C Phospholipases/genetics,metabolism
Chemicals
Isoenzymes Oligonucleotide Probes Receptors, Cell Surface Receptors, Fibroblast Growth Factor Fibroblast Growth Factors DNA Protein-Tyrosine Kinases Type C Phospholipases
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Burgess W H
American Red Cross, Rockville, Maryland 20855.
Dionne C A
Kaplow J
Mudd R
Friesel R
Zilberstein A
Schlessinger J
Jaye M
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.9.4770-4777.1990
Published
1990-09-00
Pages
4770-7
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361079
Subset
IM
Grants
NHLBI NIH HHS · HL 32398 · United States
NHLBI NIH HHS · HL 35627 · United States
NHLBI NIH HHS · HL 35762 · United States
Databases
GENBANK
M34667
External Links
PubMed source DOI: 10.1128/mcb.10.9.4770-4777.1990 PMC Full Text View journal details
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