Home LiteratureArticle Details
PMID: 216901 Published · ppublish English Journal Article

Eukaryotic ribosomal proteins stimulate Escherichia coli stringent factor to synthesize guanosine 5'-diphosphate, 3'-diphosphate (ppGpp) and guanosine 5'-triphosphate, 3'-diphosphate (ppGpp).

Molecular & general genetics : MGG ·Vol. 166 ·No. 3 ·1978-11-09 ·Pages 291-7

Martini O, Richter D

Abstract

When supplemented with Escherichia coli stringent factor, 80S ribosomes from various sources failed to support guanosine tetra- and pentaphosphate ((p)ppGpp) synthesis. In contrast, ribosomal proteins from 80S, 60S or 40S particles (mouse embryos, rabbit reticulocytes) crossreacted with the E. coli stringent factor. Significant stimulation of (p)ppGpp synthesis was achieved with a concentration as low as 5 micrograms of ribosomal proteins/ml. These observations may provide additional crtieria to detect homologies between eukaryotic and prokaryotic ribosomal proteins.

MeSH Terms
Bacterial Proteins/pharmacology Cross Reactions Escherichia coli/enzymology Guanine Nucleotides/biosynthesis Guanosine Pentaphosphate/biosynthesis Guanosine Tetraphosphate/biosynthesis Guanosine Triphosphate Phosphoric Acids Phosphotransferases/pharmacology Protein Biosynthesis Ribosomal Proteins/pharmacology Transcription, Genetic
Chemicals
Bacterial Proteins Guanine Nucleotides Phosphoric Acids Ribosomal Proteins Guanosine Tetraphosphate Guanosine Pentaphosphate Guanosine Triphosphate Phosphotransferases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Martini O
Richter D
References (29)
29 references, click to expand
  1. Synthesis of guanosine tetraphosphate (magic spot I) in Saccharomyces cerevisiae.
    Biochem Biophys Res Commun. 1977 Jan 10;74(1):314-22 PMID: 319793
  2. Studies on the interchangeability of one of the mammalian and bacterial supernatant factors in protein biosynthesis.
    J Biol Chem. 1969 Nov 25;244(22):6117-23 PMID: 4900509
  3. Reversibility of the pyrophosphoryl transfer from ATP to GTP by Escherichia coli stringent factor.
    Proc Natl Acad Sci U S A. 1974 Sep;71(9):3470-3 PMID: 4372621
  4. Escherichia coli stringent factor binds to ribosomes at a site different from that of elongation factor Tu or G.
    Biochemistry. 1975 Oct 7;14(20):4414-20 PMID: 1100104
  5. Separation of mitochondrial and cytoplasmic peptide chain elongation factors from yeast.
    Biochemistry. 1970 Dec 22;9(26):5065-70 PMID: 4921543
  6. mRNA-dependent in vitro synthesis of ribosomal proteins L12 and L10 and elongation factor Tu.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5387-91 PMID: 341155
  7. Synthesis of ppGpp by mouse embryonic ribosomes.
    Cell. 1974 Nov;3(3):249-53 PMID: 4611645
  8. Phosphorylation of rabbit reticulocyte ribosomal proteins in vitro.
    Biochim Biophys Acta. 1973 Feb 21;295(2):621-9 PMID: 4699579
  9. The control of ribonucleic acid synthesis in Escherichia coli. V. Characterization of a nucleotide associated with the stringent response.
    J Biol Chem. 1970 May 10;245(9):2309-18 PMID: 4315151
  10. Ribosomal proteins of Escherichia coli that stimulate stringent-factor-mediated pyrophosphoryl transfer in vitro.
    Proc Natl Acad Sci U S A. 1976 Jun;73(6):1839-43 PMID: 778846
  11. Ribosome specificity for the formation of guanosine polyphosphates.
    Biochem Biophys Res Commun. 1974 Feb 4;56(3):611-6 PMID: 4597065
  12. Isolation and characterization of two acidic proteins of 60s ribosomes from Artemia salina cysts.
    Proc Natl Acad Sci U S A. 1975 Dec;72(12):4744-8 PMID: 1061068
  13. Guanosine tetra- and pentaphosphate synthesis by bacterial stringent factor and eukaryotic ribosomes.
    Nature. 1977 May 26;267(5609):371-3 PMID: 194166
  14. The extraction of proteins from eukaryotic ribosomes and ribosomal subunits.
    Mol Gen Genet. 1974;135(2):97-112 PMID: 4457758
  15. Molecular weight distribution of ribosomal proteins from several vertebrate species.
    Mol Gen Genet. 1976 Dec 31;142(4):317-31 PMID: 1272245
  16. MSI and MSII made on ribosome in idling step of protein synthesis.
    Nature. 1972 Aug 18;238(5364):381-4 PMID: 4559580
  17. Formation of guanosine tetraphosphate (magic spot I) in homologous and heterologous systems.
    FEBS Lett. 1973 Aug 15;34(2):291-4 PMID: 4355915
  18. DNA-dependent in vitro synthesis of fibosomal proteins, protein elongation factors, and RNA polymerase subunit alpha: inhibition by ppGpp.
    Cell. 1976 Nov;9(3):439-48 PMID: 991274
  19. Questioning of reported evidence for guanosine tetraphosphate synthesis in a ribosome system from mouse embryos.
    Cell. 1977 Dec;12(4):1127-31 PMID: 340046
  20. Effects of guanosine tetraphosphate on cell-free synthesis of Escherichia coli ribosomal RNA and other gene products.
    Proc Natl Acad Sci U S A. 1975 Aug;72(8):2881-5 PMID: 1103124
  21. Studies on the in vitro synthesis of ppGpp and pppGpp.
    Biochim Biophys Acta. 1975 Jun 2;395(1):80-90 PMID: 806304
  22. Initiation of mammalian protein synthesis: the importance of ribosome and initiation factor quality for the efficiency of in vitro systems.
    J Mol Biol. 1973 Feb 19;73(3):329-49 PMID: 4686199
  23. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  24. The mechanism of protein synthesis-initiation, elongation and termination in translation of genetic messeges.
    Curr Top Microbiol Immunol. 1977;76:83-125 PMID: 334484
  25. Dissociation of mammalian polyribosomes into subunits by puromycin.
    Proc Natl Acad Sci U S A. 1971 Feb;68(2):390-4 PMID: 5277091
  26. Purification and properties of stringent factor.
    J Biol Chem. 1975 Feb 25;250(4):1212-7 PMID: 163249
  27. Nonribosomal synthesis of guanosine 5',3'-polyphosphates by the ribosomal wash of stringent Escherichia coli.
    Proc Natl Acad Sci U S A. 1973 Jul;70(7):2145-8 PMID: 4579015
  28. The synthesis of eucaryotic ribosomal proteins in vitro.
    Cell. 1977 May;11(1):201-12 PMID: 326416
  29. Mouse embryos fail to synthesize detectable quantities of guanosine 5'-diphosphate 3'-diphosphate.
    Dev Biol. 1977 Mar;56(1):200-5 PMID: 320067
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00267621
Published
1978-11-09
Pages
291-7
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00267621
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2