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PMID: 2216777 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A distal dimerization domain is essential for DNA-binding by the atypical HNF1 homeodomain.

Nucleic acids research ·Vol. 18 ·No. 19 ·1990-10-11 ·Pages 5853-63

Chouard T, Blumenfeld M, Bach I, Vandekerckhove J, Cereghini S, Yaniv M

Abstract

Hepatic Nuclear Factor 1 (HNF1, also referred to as LFB1, HP1 or APF) is a liver-specific transcription factor required for the expression of many hepatocyte specific genes. We report here the purification of this rat liver nuclear protein and the cloning of its cDNA using a PCR-derived approach. Seven independent clones reveal 3 alternative polyadenylation sites and a unique open reading frame. Both a motif homologous to the homeodomain and a distal dimerization domain are required for specific DNA binding. Sequence comparisons reveal several atypical features at key positions in the segment corresponding to helices III and IV of the Antaennapedia homeodomain as well as a potential 24 amino acid loop in place of the universal turn between helices II and III. Together with its property to dimerize in the presence or absence of DNA, these features place HNF1 as the prototype of a novel subclass of transcription factors distantly related to homeoproteins.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Blotting, Northern Cloning, Molecular DNA-Binding Proteins/genetics,metabolism Hepatocyte Nuclear Factor 1 Hepatocyte Nuclear Factor 1-alpha Hepatocyte Nuclear Factor 1-beta Molecular Sequence Data Nuclear Proteins Open Reading Frames Poly A Polymerase Chain Reaction Protein Biosynthesis Rats Sequence Homology, Nucleic Acid Transcription Factors/genetics,metabolism
Chemicals
DNA-Binding Proteins Hepatocyte Nuclear Factor 1-alpha Hnf1a protein, rat Nuclear Proteins Transcription Factors Hepatocyte Nuclear Factor 1 Hepatocyte Nuclear Factor 1-beta Poly A
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Chouard T
UA 1149, CNRS, Département de Biologie Moléculaire, Institut Pasteur, Paris, France.
Blumenfeld M
Bach I
Vandekerckhove J
Cereghini S
Yaniv M
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/18.19.5853
Published
1990-10-11
Pages
5853-63
Language
English
Region
England
NLM ID
0411011
PMCID
PMC332325
Subset
IM
Databases
GENBANK
X54423
External Links
PubMed source DOI: 10.1093/nar/18.19.5853 PMC Full Text View journal details
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