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PMID: 22844245 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The Arabidopsis HEI10 is a new ZMM protein related to Zip3.

PLoS genetics ·Vol. 8 ·No. 7 ·2012-00-00 ·Pages e1002799

Chelysheva L, Vezon D, Chambon A, Gendrot G, Pereira L, Lemhemdi A, Vrielynck N, Le Guin S, Novatchkova M, Grelon M

Abstract

In numerous species, the formation of meiotic crossovers is largely under the control of a group of proteins known as ZMM. Here, we identified a new ZMM protein, HEI10, a RING finger-containing protein that is well conserved among species. We show that HEI10 is structurally and functionally related to the yeast Zip3 ZMM and that it is absolutely required for class I crossover (CO) formation in Arabidopsis thaliana. Furthermore, we show that it is present as numerous foci on the chromosome axes and the synaptonemal complex central element until pachytene. Then, from pachytene to diakinesis, HEI10 is retained at a limited number of sites that correspond to class I COs, where it co-localises with MLH1. Assuming that HEI10 early staining represents an early selection of recombination intermediates to be channelled into the ZMM pathway, HEI10 would therefore draw a continuity between early chosen recombination intermediates and final class I COs.

MeSH Terms
Adenosine Triphosphatases/genetics Amino Acid Sequence Arabidopsis/cytology,genetics Arabidopsis Proteins/genetics Chromosomal Proteins, Non-Histone/genetics Chromosomes, Plant/genetics Crossing Over, Genetic Fertility/genetics Homologous Recombination Miosis/genetics Molecular Sequence Data MutL Protein Homolog 1 Mutation RING Finger Domains/genetics Saccharomyces cerevisiae Proteins/genetics Sequence Homology, Amino Acid Synaptonemal Complex/genetics Ubiquitin-Protein Ligases/genetics Yeasts/genetics
Chemicals
Arabidopsis Proteins Chromosomal Proteins, Non-Histone HEI10 protein, Arabidopsis Saccharomyces cerevisiae Proteins Ubiquitin-Protein Ligases Adenosine Triphosphatases MLH1 protein, Arabidopsis MutL Protein Homolog 1 Zip3 protein, S cerevisiae
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Chelysheva Liudmila
INRA, UMR1318, Institut Jean-Pierre Bourgin, RD10, Versailles, France.
Vezon Daniel
Chambon Aurélie
Gendrot Ghislaine
Pereira Lucie
Lemhemdi Afef
Vrielynck Nathalie
Le Guin Sylvia
Novatchkova Maria
Grelon Mathilde
Conflict of Interest

The authors have declared that no competing interests exist.

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Article Info
Journal
PLoS genetics
Abbr.
PLoS Genet
ISSN
1553-7404
DOI
10.1371/journal.pgen.1002799
Published
2012-00-00
Epub
2012-00-26
Pages
e1002799
Language
English
Region
United States
NLM ID
101239074
PMCID
PMC3405992
Subset
IM
External Links
PubMed source DOI: 10.1371/journal.pgen.1002799 PMC Full Text View journal details
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