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PMID: 22891265 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A steep phosphoinositide bis-phosphate gradient forms during fungal filamentous growth.

The Journal of cell biology ·Vol. 198 ·No. 4 ·2012-08-20 ·Pages 711-30

Vernay A, Schaub S, Guillas I, Bassilana M, Arkowitz RA

Abstract

Membrane lipids have been implicated in many critical cellular processes, yet little is known about the role of asymmetric lipid distribution in cell morphogenesis. The phosphoinositide bis-phosphate PI(4,5)P(2) is essential for polarized growth in a range of organisms. Although an asymmetric distribution of this phospholipid has been observed in some cells, long-range gradients of PI(4,5)P(2) have not been observed. Here, we show that in the human pathogenic fungus Candida albicans a steep, long-range gradient of PI(4,5)P(2) occurs concomitant with emergence of the hyphal filament. Both sufficient PI(4)P synthesis and the actin cytoskeleton are necessary for this steep PI(4,5)P(2) gradient. In contrast, neither microtubules nor asymmetrically localized mRNAs are critical. Our results indicate that a gradient of PI(4,5)P(2), crucial for filamentous growth, is generated and maintained by the filament tip-localized PI(4)P-5-kinase Mss4 and clearing of this lipid at the back of the cell. Furthermore, we propose that slow membrane diffusion of PI(4,5)P(2) contributes to the maintenance of such a gradient.

MeSH Terms
1-Phosphatidylinositol 4-Kinase/biosynthesis,genetics Actin Cytoskeleton/enzymology,physiology Candida albicans/genetics,growth & development,pathogenicity Cell Polarity/physiology Fungal Proteins/biosynthesis,genetics Hyphae/enzymology,growth & development Phosphatidylinositol 4,5-Diphosphate/biosynthesis,metabolism Phosphotransferases (Alcohol Group Acceptor)/biosynthesis,genetics
Chemicals
Fungal Proteins Phosphatidylinositol 4,5-Diphosphate Phosphotransferases (Alcohol Group Acceptor) 1-Phosphatidylinositol 4-Kinase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Vernay Aurélia
Institute of Biology Valrose, Université Nice - Sophia Antipolis, 06108 Nice Cedex 2, France.
Schaub Sébastien
Guillas Isabelle
Bassilana Martine
Arkowitz Robert A
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
1540-8140
DOI
10.1083/jcb.201203099
Published
2012-08-20
Epub
2012-00-13
Pages
711-30
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC3514036
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.201203099 PMC Full Text View journal details
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