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PMID: 2294409 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Estradiol and estrogen receptor-dependent stabilization of a minivitellogenin mRNA lacking 5,100 nucleotides of coding sequence.

Molecular and cellular biology ·Vol. 10 ·No. 1 ·1990-01-00 ·Pages 371-6

Nielsen DA, Shapiro DJ

Abstract

We have developed a transfection assay to investigate the estrogen-mediated stabilization of cytoplasmic vitellogenin mRNA. A minivitellogenin (MV5) gene containing the 5' and 3' untranslated and coding regions but lacking 5,075 nucleotides of internal coding sequence was constructed. Cotransfection of the MV5 plasmid and a Xenopus estrogen receptor expression plasmid into Xenopus liver tissue culture cells yielded a 529-nucleotide MV5 mRNA, which was specifically stabilized by estrogen. MV5 mRNA exhibited the increased stability indicative of positive regulation when the estradiol-estrogen receptor complex was present and was not destabilized by unliganded estrogen receptor. Transfected estrogen receptor, estradiol, and 529 nucleotides of the 5,604-nucleotide vitellogenin B1 mRNA were sufficient for stabilization.

MeSH Terms
Animals Cloning, Molecular Estradiol/pharmacology Gene Expression Regulation/drug effects Oocytes/physiology RNA, Messenger/genetics Receptors, Estrogen/physiology Restriction Mapping Structure-Activity Relationship Transfection Vitellogenins/genetics Xenopus laevis
Chemicals
RNA, Messenger Receptors, Estrogen Vitellogenins Estradiol
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Nielsen D A
Department of Biochemistry, University of Illinois, Urbana 61801.
Shapiro D J
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.1.371-376.1990
Published
1990-01-00
Pages
371-6
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360757
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.10.1.371-376.1990 PMC Full Text View journal details
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